Transcript: Human NM_001004105.3

Homo sapiens G protein-coupled receptor kinase 6 (GRK6), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
GRK6 (2870)
Length:
1956
CDS:
171..1853

Additional Resources:

NCBI RefSeq record:
NM_001004105.3
NBCI Gene record:
GRK6 (2870)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145803 GGCCACGCTCAGGTACTCGT pXPR_003 GGG 451 27% 6 1.0421 GRK6 GRK6 75702
2 BRDN0001146643 GTGCTGACACTGATGAACGG pXPR_003 GGG 800 48% 9 0.8416 GRK6 GRK6 75704
3 BRDN0001147105 AAGTGACCCCGGATGACAAG pXPR_003 CGG 288 17% 4 0.1823 GRK6 GRK6 75705
4 BRDN0001146204 CTGCGATCATCTCGTACAGG pXPR_003 AGG 1124 67% 12 -0.0606 GRK6 GRK6 75703
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001004105.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199094 CCGCTCACTTTGCTCACAGCT pLKO.1 1418 CDS 100% 0.880 1.232 N GRK6 n/a
2 TRCN0000199727 GCGGCAGCTAACGCAGAATTT pLKO.1 476 CDS 100% 13.200 9.240 N GRK6 n/a
3 TRCN0000001367 CCTCGACAGCATCTACTTCAA pLKO.1 653 CDS 100% 4.950 2.970 N GRK6 n/a
4 TRCN0000001369 CAGTAGGTTTGTAGTGAGCTT pLKO.1 896 CDS 100% 2.640 1.584 N GRK6 n/a
5 TRCN0000001368 CAGCATCTACTTCAACCGTTT pLKO.1 659 CDS 100% 4.050 2.025 Y GRK6 n/a
6 TRCN0000010619 CTGAATGTCTTTGGGCTGGAT pLKO.1 1740 CDS 100% 2.640 1.320 Y GRK6 n/a
7 TRCN0000199994 CGAGGAGTATTCCGAGCGCTT pLKO.1 1385 CDS 100% 0.720 0.360 Y GRK6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001004105.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00682 pDONR223 100% 96.9% 97% None 1678_1679delAG;1680_1681ins50 n/a
2 ccsbBroad304_00682 pLX_304 0% 96.9% 97% V5 1678_1679delAG;1680_1681ins50 n/a
3 TRCN0000480454 ACTTTTCCCGCTTGTCCTGCCCCC pLX_317 24.5% 96.9% 97% V5 1678_1679delAG;1680_1681ins50 n/a
4 ccsbBroadEn_06322 pDONR223 100% 94.9% 94.9% None (many diffs) n/a
5 ccsbBroad304_06322 pLX_304 0% 94.9% 94.9% V5 (many diffs) n/a
6 TRCN0000479803 TGAGACGCGACATGGTATATCCAG pLX_317 20.5% 94.9% 94.9% V5 (many diffs) n/a
7 ccsbBroadEn_14658 pDONR223 0% 94.9% 94.9% None (many diffs) n/a
8 ccsbBroad304_14658 pLX_304 0% 94.9% 94.9% V5 (many diffs) n/a
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