Construct: ORF TRCN0000479803
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007297.2_s317c1
- Derived from:
- ccsbBroadEn_06322
- DNA Barcode:
- TGAGACGCGACATGGTATATCCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRK6 (2870)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479803
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2870 | GRK6 | G protein-coupled receptor ... | NM_002082.3 | 99.8% | 99.8% | 1120C>T;1392G>C;1566A>G |
| 2 | human | 2870 | GRK6 | G protein-coupled receptor ... | NM_001004106.3 | 97.6% | 95.4% | (many diffs) |
| 3 | human | 2870 | GRK6 | G protein-coupled receptor ... | NM_001004105.3 | 94.9% | 94.9% | (many diffs) |
| 4 | human | 2870 | GRK6 | G protein-coupled receptor ... | XM_006714859.2 | 94.3% | 94.3% | (many diffs) |
| 5 | human | 2870 | GRK6 | G protein-coupled receptor ... | XM_006714858.3 | 93.4% | 91.2% | (many diffs) |
| 6 | human | 2870 | GRK6 | G protein-coupled receptor ... | XM_011534537.2 | 91.5% | 82.2% | (many diffs) |
| 7 | human | 2870 | GRK6 | G protein-coupled receptor ... | XM_024446032.1 | 64.1% | 64.1% | (many diffs) |
| 8 | human | 2870 | GRK6 | G protein-coupled receptor ... | NM_001364164.2 | 61.9% | 59.7% | (many diffs) |
| 9 | human | 2870 | GRK6 | G protein-coupled receptor ... | XM_011534538.1 | 54.8% | 54.8% | (many diffs) |
| 10 | human | 2871 | GRK6P1 | G protein-coupled receptor ... | NR_144554.1 | 53.3% | (many diffs) | |
| 11 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_001038018.4 | 89.5% | 95.7% | (many diffs) |
| 12 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_001286064.1 | 88.7% | 94.9% | (many diffs) |
| 13 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_001112711.2 | 87.4% | 92.1% | (many diffs) |
| 14 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | XM_006517251.3 | 87.4% | 92.8% | (many diffs) |
| 15 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_011938.4 | 84.8% | 91.6% | (many diffs) |
| 16 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_001286063.1 | 84.3% | 90.3% | (many diffs) |
| 17 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_001286065.1 | 82.1% | 86.4% | (many diffs) |
| 18 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | XM_006517252.1 | 49% | 52.2% | (many diffs) |
| 19 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_001286066.1 | 44.4% | 48.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1833
- ORF length:
- 1767
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctcgagaac atcgtagcga acacggtgct actcaaggcc cgggaaggtg 121 gcggtggaaa tcgcaaaggc aaaagcaaga aatggcggca gatgctccag ttccctcaca 181 tcagccagtg cgaagagctg cggctcagcc tcgagcgtga ctatcacagc ctgtgcgagc 241 ggcagcccat tgggcgcctg ctgttccgag agttctgtgc cacgaggccg gagctgagcc 301 gctgcgtcgc cttcctggat ggggtggccg agtatgaagt gaccccggat gacaagcgga 361 aggcatgtgg gcggcagcta acgcagaatt ttctgagcca cacgggtcct gacctcatcc 421 ctgaggtccc ccggcagctg gtgacgaact gcacccagcg gctggagcag ggtccctgca 481 aagacctttt ccaggaactc acccggctga cccacgagta cctgagcgtg gccccttttg 541 ccgactacct cgacagcatc tacttcaacc gtttcctgca gtggaagtgg ctggaaaggc 601 agccagtgac caaaaacacc ttcaggcaat accgagtcct gggcaaaggt ggctttgggg 661 aggtgtgcgc ctgccaggtg cgggccacag gtaagatgta tgcctgcaag aagctagaga 721 aaaagcggat caagaagcgg aaaggggagg ccatggcgct gaacgagaag cagatcctgg 781 agaaagtgaa cagtaggttt gtagtgagct tggcctacgc ctatgagacc aaggacgcgc 841 tgtgcctggt gctgacactg atgaacgggg gcgacctcaa gttccacatc taccacatgg 901 gccaggctgg cttccccgaa gcgcgggccg tcttctacgc cgccgagatc tgctgtggcc 961 tggaggacct gcaccgggag cgcatcgtgt acagggacct gaagcccgag aacatcttgc 1021 tggatgacca cggccacatc cgcatctctg acctgggact agctgtgcat gtgcccgagg 1081 gccagaccat caaagggcgt gtgggcaccg tgggttacat ggctccggag gtggtgaaga 1141 atgaacggta cacgttcagc cctgactggt gggcgctcgg ctgcttcctg tacgagatga 1201 tcgcaggcca gtcgcccttc cagcagagga agaagaagat caagcgggag gaggtggagc 1261 ggctggtgaa ggaggtcccc gaggagtatt ccgagcgctt ttccccgcag gcccgctcac 1321 tttgctcaca gctcctctgc aaggaccctg ccgaacgcct ggggtgtcgt gggggcagtg 1381 cccgcgaggt gaaggagcac cccctcttta agaagctgaa cttcaagcgg ctgggagctg 1441 gcatgctgga gccgcccttc aagcctgacc cccaggCCAT TTACTGCAAG GATGTTCTGG 1501 ACATTGAACA GTTCTCTACG GTCAAGGGCG TGGAGCTGGA GCCTACCGAC CAGGACTTCT 1561 ACCAGAAGTT TGCCACAGGC AGTGTGCCCA TCCCCTGGCA GAACGAGATG GTGGAGACCG 1621 AGTGCTTCCA GGAGCTGAAT GTCTTTGGGC TGGATGGCTC AGTTCCCCCA GACCTGGACT 1681 GGAAGGGCCA GCCACCTGCA CCTCCTAAAA AGGGACTGCT GCAGAGACTC TTCAGTCGCC 1741 AAAGGATTGC TGTGGAAACT GCAGCGACAG CGAGGAAGAG CTCCCCACCC GCCTCTAGCC 1801 CCCAGCCCGA GGCCCCCACC AGCAGTTGGC GGTACCCAAC TTTCTTGTAC AAAGTGGTTG 1861 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1921 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 1981 AGACGCGACA TGGTATATCC AGACGCGTTA AGTCgacaat caacctctgg attacaaaat 2041 ttgtgaaaga tt