Construct: ORF TRCN0000480454
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001558.1_s317c1
- Derived from:
- ccsbBroadEn_00682
- DNA Barcode:
- ACTTTTCCCGCTTGTCCTGCCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GRK6 (2870)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480454
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2870 | GRK6 | G protein-coupled receptor ... | NM_001004106.3 | 100% | 100% | |
| 2 | human | 2870 | GRK6 | G protein-coupled receptor ... | NM_002082.3 | 97.7% | 95.5% | 1678_1679delAG;1731_1767del |
| 3 | human | 2870 | GRK6 | G protein-coupled receptor ... | NM_001004105.3 | 96.9% | 97% | 1678_1679delAG;1680_1681ins50 |
| 4 | human | 2870 | GRK6 | G protein-coupled receptor ... | XM_006714859.2 | 92.3% | 90.1% | 0_1ins96;1582_1583delAG;1635_1671del |
| 5 | human | 2870 | GRK6 | G protein-coupled receptor ... | XM_006714858.3 | 91.3% | 87% | (many diffs) |
| 6 | human | 2870 | GRK6 | G protein-coupled receptor ... | XM_011534537.2 | 89.5% | 78.1% | (many diffs) |
| 7 | human | 2870 | GRK6 | G protein-coupled receptor ... | NM_001364164.2 | 63.5% | 63.5% | 0_1ins630 |
| 8 | human | 2870 | GRK6 | G protein-coupled receptor ... | XM_024446032.1 | 62.1% | 59.9% | 0_1ins630;1048_1049delAG;1101_1137del |
| 9 | human | 2870 | GRK6 | G protein-coupled receptor ... | XM_011534538.1 | 52.8% | 50.5% | 0_1ins795;883_884delAG;936_972del |
| 10 | human | 2871 | GRK6P1 | G protein-coupled receptor ... | NR_144554.1 | 52.1% | (many diffs) | |
| 11 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_001112711.2 | 89.4% | 96.7% | (many diffs) |
| 12 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_001038018.4 | 87.4% | 92% | (many diffs) |
| 13 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_001286064.1 | 86.7% | 91.1% | (many diffs) |
| 14 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_011938.4 | 86.5% | 93.7% | (many diffs) |
| 15 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | XM_006517251.3 | 85.4% | 89.1% | (many diffs) |
| 16 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_001286065.1 | 84% | 90.7% | (many diffs) |
| 17 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_001286063.1 | 82.3% | 86.5% | (many diffs) |
| 18 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | XM_006517252.1 | 47% | 48.5% | (many diffs) |
| 19 | mouse | 26385 | Grk6 | G protein-coupled receptor ... | NM_001286066.1 | 45.2% | 49.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1794
- ORF length:
- 1728
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctcgagaac atcgtagcga acacggtgct actcaaggcc cgggaaggtg 121 gcggtggaaa tcgcaaaggc aaaagcaaga aatggcggca gatgctccag ttccctcaca 181 tcagccagtg cgaagagctg cggctcagcc tcgagcgtga ctatcacagc ctgtgcgagc 241 ggcagcccat tgggcgcctg ctgttccgag agttctgtgc cacgaggccg gagctgagcc 301 gctgcgtcgc cttcctggat ggggtggccg agtatgaagt gaccccggat gacaagcgga 361 aggcatgtgg gcggcagcta acgcagaatt ttctgagcca cacgggtcct gacctcatcc 421 ctgaggtccc ccggcagctg gtgacgaact gcacccagcg gctggagcag ggtccctgca 481 aagacctttt ccaggaactc acccggctga cccacgagta cctgagcgtg gccccttttg 541 ccgactacct cgacagcatc tacttcaacc gtttcctgca gtggaagtgg ctggaaaggc 601 agccagtgac caaaaacacc ttcaggcaat accgagtcct gggcaaaggt ggctttgggg 661 aggtgtgcgc ctgccaggtg cgggccacag gtaagatgta tgcctgcaag aagctagaga 721 aaaagcggat caagaagcgg aaaggggagg ccatggcgct gaacgagaag cagatcctgg 781 agaaagtgaa cagtaggttt gtagtgagct tggcctacgc ctatgagacc aaggacgcgc 841 tgtgcctggt gctgacactg atgaacgggg gcgacctcaa gttccacatc taccacatgg 901 gccaggctgg cttccccgaa gcgcgggccg tcttctacgc cgccgagatc tgctgtggcc 961 tggaggacct gcaccgggag cgcatcgtgt acagggacct gaagcccgag aacatcttgc 1021 tggatgacca cggccacatc cgcatctctg acctgggact agctgtgcat gtgcccgagg 1081 gccagaccat caaagggcgt gtgggcaccg tgggttacat ggctccggag gtggtgaaga 1141 atgaacggta cacgttcagc cctgactggt gggcgctcgg ctgcctcctg tacgagatga 1201 tcgcaggcca gtcgcccttc cagcagagga agaagaagat caagcgggag gaggtggagc 1261 ggctggtgaa ggaggtcccc gaggagtatt ccgagcgctt ttccccgcag gcccgctcac 1321 tttgctcaca gctcctctgc aaggaccctg ccgaacgcct ggggtgtcgt gggggCAGTG 1381 CCCGCGAGGT GAAGGAGCAC CCCCTCTTTA AGAAGCTGAA CTTCAAGCGG CTGGGAGCTG 1441 GCATGCTGGA GCCGCCGTTC AAGCCTGACC CCCAGGCCAT TTACTGCAAG GATGTTCTGG 1501 ACATTGAACA GTTCTCTACG GTCAAGGGCG TGGAGCTGGA GCCTACCGAC CAGGACTTCT 1561 ACCAGAAGTT TGCCACAGGC AGTGTGCCCA TCCCCTGGCA GAACGAGATG GTGGAGACCG 1621 AGTGCTTCCA AGAGCTGAAT GTCTTTGGGC TGGATGGCTC AGTTCCCCCA GACCTGGACT 1681 GGAAGGGCCA GCCACCTGCA CCTCCTAAAA AGGGACTGCT GCAGAGACTC TTCAGTCGCC 1741 AAGATTGCTG TGGAAACTGC AGCGACAGCG AGGAAGAGCT CCCCACCCGC CTCTACCCAA 1801 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1861 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1921 TATCTTGTGG AAAGGACGAA CTTTTCCCGC TTGTCCTGCC CCCACGCGTT AAGTCgacaa 1981 tcaacctctg gattacaaaa tttgtgaaag att