Transcript: Mouse NM_001013792.2

Mus musculus OTU deubiquitinase with linear linkage specificity (Otulin), mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Otulin (432940)
Length:
1491
CDS:
85..1143

Additional Resources:

NCBI RefSeq record:
NM_001013792.2
NBCI Gene record:
Otulin (432940)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001013792.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000194367 CTGAGGATGATCGGCACTATA pLKO.1 1085 CDS 100% 13.200 18.480 N Otulin n/a
2 TRCN0000347228 CTGAGGATGATCGGCACTATA pLKO_005 1085 CDS 100% 13.200 18.480 N Otulin n/a
3 TRCN0000217453 GAAGACTAGCTGTTGATAATG pLKO.1 1276 3UTR 100% 13.200 18.480 N Otulin n/a
4 TRCN0000217684 GAGCCAACTTTGGACCATTAT pLKO.1 1250 3UTR 100% 13.200 18.480 N Otulin n/a
5 TRCN0000193865 CCTGATGTTAAACAGAGCCAT pLKO.1 786 CDS 100% 2.640 3.696 N Otulin n/a
6 TRCN0000347227 CCTGATGTTAAACAGAGCCAT pLKO_005 786 CDS 100% 2.640 3.696 N Otulin n/a
7 TRCN0000174588 GAGAAGCTGATAAACAAGTAT pLKO.1 559 CDS 100% 5.625 3.938 N Otulin n/a
8 TRCN0000194315 CCTCTACGAAGCTGTGAAGTT pLKO.1 765 CDS 100% 4.950 3.465 N Otulin n/a
9 TRCN0000347300 CCTCTACGAAGCTGTGAAGTT pLKO_005 765 CDS 100% 4.950 3.465 N Otulin n/a
10 TRCN0000176217 GCTCATACTGTTACCAGAGAA pLKO.1 543 CDS 100% 4.950 3.465 N Otulin n/a
11 TRCN0000216202 CTCAGAAATTCACTTCCATAA pLKO.1 425 CDS 100% 10.800 6.480 N Otulin n/a
12 TRCN0000176402 CAGTGGAAACTTGGACTGAAA pLKO.1 592 CDS 100% 4.950 2.970 N Otulin n/a
13 TRCN0000347226 CAGTGGAAACTTGGACTGAAA pLKO_005 592 CDS 100% 4.950 2.970 N Otulin n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001013792.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12935 pDONR223 100% 74.4% 77.8% None (many diffs) n/a
2 ccsbBroad304_12935 pLX_304 0% 74.4% 77.8% V5 (many diffs) n/a
3 TRCN0000471981 TAGCCGCTGCACCAATTTCGCGCC pLX_317 51.3% 74.4% 77.8% V5 (many diffs) n/a
4 ccsbBroadEn_12936 pDONR223 100% 52.4% 54.5% None (many diffs) n/a
5 ccsbBroad304_12936 pLX_304 0% 52.4% 54.5% V5 (many diffs) n/a
6 TRCN0000475487 CTCTTATATACGTTTCCCCCTAAT pLX_317 33% 52.4% 54.5% V5 (many diffs) n/a
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