Transcript: Human NM_001014450.3

Homo sapiens small proline rich protein 2F (SPRR2F), mRNA.

Source:
NCBI, updated 2019-05-04
Taxon:
Homo sapiens (human)
Gene:
SPRR2F (6705)
Length:
675
CDS:
63..281

Additional Resources:

NCBI RefSeq record:
NM_001014450.3
NBCI Gene record:
SPRR2F (6705)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001014450.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000188495 CACCATCAAAGTGTCCACAGT pLKO.1 172 CDS 100% 2.640 1.584 N SPRR2F n/a
2 TRCN0000188388 CATCAAAGTGTCCACAGTCCT pLKO.1 175 CDS 100% 2.640 1.584 N SPRR2F n/a
3 TRCN0000163447 GCTTCTTTCCTCTTAGCCTGT pLKO.1 390 3UTR 100% 2.160 1.296 N SPRR2F n/a
4 TRCN0000158926 GCATGAAAGGATAAGGATAAT pLKO.1 303 3UTR 100% 13.200 6.600 Y SPRR2F n/a
5 TRCN0000255629 GTCCACCCAAGAGCAAGTAAC pLKO_005 262 CDS 100% 10.800 5.400 Y SPRR2D n/a
6 TRCN0000203685 CATCAGGAGCATGAAAGGATA pLKO.1 295 3UTR 100% 4.950 2.475 Y SPRR2F n/a
7 TRCN0000164565 CCACCTGCATCTTCTCATCAA pLKO.1 346 3UTR 100% 4.950 2.475 Y SPRR2F n/a
8 TRCN0000243250 GTAACAGCTTCAGGATTCATC pLKO_005 278 CDS 100% 4.950 2.475 Y SPRR2B n/a
9 TRCN0000255628 TAACAGCTTCAGGATTCATCA pLKO_005 279 CDS 100% 4.950 2.475 Y SPRR2D n/a
10 TRCN0000203973 GCATCTTCTCATCAAAGCCAT pLKO.1 352 3UTR 100% 2.640 1.320 Y SPRR2F n/a
11 TRCN0000155726 CAGCAGTGCCAGCAGAAATAT pLKO.1 204 CDS 100% 15.000 7.500 Y SPRR2A n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001014450.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06991 pDONR223 100% 96.2% 91.6% None (many diffs) n/a
2 ccsbBroad304_06991 pLX_304 0% 96.2% 91.6% V5 (many diffs) n/a
3 TRCN0000467715 TCCTGGGTCCGAGCTCTAAATTCC pLX_317 100% 96.2% 91.6% V5 (many diffs) n/a
Download CSV