Construct: ORF TRCN0000467715
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018444.1_s317c1
- Derived from:
- ccsbBroadEn_06991
- DNA Barcode:
- TCCTGGGTCCGAGCTCTAAATTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPRR2A (6700)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467715
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6700 | SPRR2A | small proline rich protein 2A | NM_005988.3 | 99.5% | 100% | 75G>A |
| 2 | human | 6701 | SPRR2B | small proline rich protein 2B | NM_001017418.3 | 99% | 98.6% | 75G>A;193C>T |
| 3 | human | 6701 | SPRR2B | small proline rich protein 2B | XM_017002174.1 | 99% | 98.6% | 75G>A;193C>T |
| 4 | human | 6703 | SPRR2D | small proline rich protein 2D | NM_006945.5 | 97.6% | 95.8% | (many diffs) |
| 5 | human | 6703 | SPRR2D | small proline rich protein 2D | XM_017002175.1 | 97.6% | 95.8% | (many diffs) |
| 6 | human | 6704 | SPRR2E | small proline rich protein 2E | NM_001024209.4 | 97.2% | 95.8% | (many diffs) |
| 7 | human | 6705 | SPRR2F | small proline rich protein 2F | NM_001014450.3 | 96.2% | 91.6% | (many diffs) |
| 8 | human | 6705 | SPRR2F | small proline rich protein 2F | XM_017002176.1 | 96.2% | 91.6% | (many diffs) |
| 9 | human | 6706 | SPRR2G | small proline rich protein 2G | NM_001014291.4 | 85.3% | 82.1% | (many diffs) |
| 10 | human | 6706 | SPRR2G | small proline rich protein 2G | XM_017002177.1 | 85.3% | 82.1% | (many diffs) |
| 11 | human | 6706 | SPRR2G | small proline rich protein 2G | XM_017002178.1 | 85.3% | 82.1% | (many diffs) |
| 12 | human | 6702 | SPRR2C | small proline rich protein ... | NR_003062.1 | 29.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 216
- ORF end:
- 432
- ORF length:
- 216
- Sequence:
-
1 TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA 61 CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA 121 GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GCTAGCATCG 181 ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCACCATGTC TTATCAACAG CAGCAGTGCA 241 AGCAGCCCTG CCAGCCACCT CCTGTGTGCC CCACGCCAAA GTGCCCAGAA CCATGTCCAC 301 CCCCGAAGTG CCCTGAGCCC TGCCCACCAC CAAAGTGTCC ACAGCCCTGC CCACCTCAGC 361 AGTGCCAGCA GAAATATCCT CCTGTGACAC CTTCCCCACC CTGCCAGTCA AAGTATCCAC 421 CGAAGAGCAA GTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 481 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT TGAAAGTATT 541 TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC CTGGGTCCGA GCTCTAAATT 601 CCACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt