Transcript: Human NM_001042747.1

Homo sapiens FGR proto-oncogene, Src family tyrosine kinase (FGR), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-04
Taxon:
Homo sapiens (human)
Gene:
FGR (2268)
Length:
2501
CDS:
272..1861

Additional Resources:

NCBI RefSeq record:
NM_001042747.1
NBCI Gene record:
FGR (2268)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147903 AGCTTGGATTGAGTCAACAG pXPR_003 GGG 406 26% 5 0.6768 FGR FGR 75721
2 BRDN0001146726 GTGACCGAGTTCATGTGTCA pXPR_003 CGG 1013 64% 9 0.536 FGR FGR 75719
3 BRDN0001147671 CGAGTTGAACTGAACCCGTG pXPR_003 TGG 637 40% 7 0.3258 FGR FGR 75720
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001042747.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199966 CCCTGTTCATTGCCCTGTATG pLKO.1 510 CDS 100% 10.800 15.120 N FGR n/a
2 TRCN0000197132 GAACGCATGAACTACATTCAC pLKO.1 1391 CDS 100% 4.950 3.960 N FGR n/a
3 TRCN0000001597 ACCCTGTTCATTGCCCTGTAT pLKO.1 509 CDS 100% 4.950 3.465 N FGR n/a
4 TRCN0000001596 CACATCCTGAACAATACTGAA pLKO.1 584 CDS 100% 4.950 3.465 N FGR n/a
5 TRCN0000199582 GCGTGCAAGATCGCAGACTTT pLKO.1 1454 CDS 100% 4.950 3.465 N FGR n/a
6 TRCN0000199350 CCCTCTATTCTCTTGTGTCTG pLKO.1 2268 3UTR 100% 4.050 2.835 N FGR n/a
7 TRCN0000001595 GCATGAATAAACGGGAAGTGT pLKO.1 1653 CDS 100% 3.000 2.100 N FGR n/a
8 TRCN0000001594 CTTCCTTGATAGTGGCACCAT pLKO.1 463 CDS 100% 2.640 1.848 N FGR n/a
9 TRCN0000199653 GCCTTTCTCATTCGGGAAAGC pLKO.1 770 CDS 100% 0.405 0.284 N FGR n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001042747.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00564 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00564 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000478102 CGGACTTCGCGTGCGTAGGTCTTC pLX_317 21.4% 100% 100% V5 n/a
4 ccsbBroadEn_14641 pDONR223 0% 100% 100% None n/a
5 ccsbBroad304_14641 pLX_304 0% 100% 100% V5 n/a
6 TRCN0000472499 CTCTCTAATATTTACACGCAAGGT pLX_317 26.9% 100% 100% V5 n/a
7 TRCN0000492193 ACATCTCGAATCGGTTGGCTTTCC pLX_317 11% 100% 100% V5 (not translated due to prior stop codon) n/a
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