Transcript: Human NM_001114099.3

Homo sapiens seizure related 6 homolog like 2 (SEZ6L2), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
SEZ6L2 (26470)
Length:
3594
CDS:
532..3054

Additional Resources:

NCBI RefSeq record:
NM_001114099.3
NBCI Gene record:
SEZ6L2 (26470)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001114099.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000303652 ACTTCAGCAACCCGCTGTATG pLKO_005 2996 CDS 100% 10.800 15.120 N SEZ6L2 n/a
2 TRCN0000303653 GCTTCGTATTGCACTTCAAAG pLKO_005 2210 CDS 100% 10.800 15.120 N SEZ6L2 n/a
3 TRCN0000303651 TGCTGTTAAGCCTTCGATTTG pLKO_005 1670 CDS 100% 10.800 15.120 N SEZ6L2 n/a
4 TRCN0000063207 GCTCCAAGTTGAGATATTGAA pLKO.1 2019 CDS 100% 5.625 7.875 N SEZ6L2 n/a
5 TRCN0000063205 CGTGATCTATGATTCGGACAT pLKO.1 1566 CDS 100% 4.050 5.670 N SEZ6L2 n/a
6 TRCN0000063206 GCGGGAGTATGAAGTTTCCAT pLKO.1 3030 CDS 100% 3.000 2.400 N SEZ6L2 n/a
7 TRCN0000303654 CTGGAGATCCTACAGTAAATA pLKO_005 3204 3UTR 100% 15.000 10.500 N SEZ6L2 n/a
8 TRCN0000063203 CCAGCCCAGTTCTGTGTAATA pLKO.1 824 CDS 100% 13.200 9.240 N SEZ6L2 n/a
9 TRCN0000299670 CCAGCCCAGTTCTGTGTAATA pLKO_005 824 CDS 100% 13.200 9.240 N SEZ6L2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001114099.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08016 pDONR223 100% 87.9% 78.6% None (many diffs) n/a
2 ccsbBroad304_08016 pLX_304 0% 87.9% 78.6% V5 (many diffs) n/a
3 TRCN0000478879 GATCTATATGCGACAGGGGTCAGC pLX_317 16.2% 87.9% 78.6% V5 (many diffs) n/a
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