Construct: ORF TRCN0000478879
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015034.1_s317c1
- Derived from:
- ccsbBroadEn_08016
- DNA Barcode:
- GATCTATATGCGACAGGGGTCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEZ6L2 (26470)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478879
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26470 | SEZ6L2 | seizure related 6 homolog l... | NM_001114100.3 | 99.9% | 99.8% | 221G>C |
| 2 | human | 26470 | SEZ6L2 | seizure related 6 homolog l... | NM_012410.4 | 89.4% | 80% | (many diffs) |
| 3 | human | 26470 | SEZ6L2 | seizure related 6 homolog l... | NM_001114099.3 | 87.9% | 78.6% | (many diffs) |
| 4 | human | 26470 | SEZ6L2 | seizure related 6 homolog l... | NM_001243332.1 | 87.6% | 87.5% | 221G>C;511_852del |
| 5 | human | 26470 | SEZ6L2 | seizure related 6 homolog l... | NM_201575.4 | 86.2% | 86.1% | 221G>C;511_852del;2485_2486ins39 |
| 6 | human | 26470 | SEZ6L2 | seizure related 6 homolog l... | NM_001243333.1 | 82.8% | 82.7% | 78_79ins132;89G>C;379_720del |
| 7 | mouse | 233878 | Sez6l2 | seizure related 6 homolog l... | NM_001252567.1 | 78.6% | 78.2% | (many diffs) |
| 8 | mouse | 233878 | Sez6l2 | seizure related 6 homolog l... | NM_144926.5 | 77.6% | 84.5% | (many diffs) |
| 9 | mouse | 233878 | Sez6l2 | seizure related 6 homolog l... | NM_001252566.1 | 76.2% | 83% | (many diffs) |
| 10 | mouse | 233878 | Sez6l2 | seizure related 6 homolog l... | XM_006507737.2 | 73.5% | 80.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2493
- ORF length:
- 2427
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gactcccagg gcccagcacc cgccgcctcc ccagctgctg ttcctaattc 121 tgctgagctg tccctggatc cagggtctgc ccctgaagga ggaggagata ttgccagagc 181 ctggaagtga gacccccacg gtggcctctg aggccctggc tgaactgctt catggggccc 241 tgctgaggag gggcccagag atgggctacc tgccaggatc tgatccggac cccacgctag 301 ccacccctcc ggccggccag actctcgcag tgccctccct gccacgggcc actgagccgg 361 ggacagggcc tctgacaaca gccgtcaccc ctaacggggt caggggggca ggccccactg 421 cgccagaact gctgaccccg cccccaggaa ccacagcccc acccccaccc agccctgcct 481 ccccagggcc tccccttggg cctgagggag gagaggagga gacgacgacc accatcatca 541 ccacgacaac tgttaccact acggtgacca gcccagccta cctcctgagc tgtggcttcc 601 ctccccggcc ggcccatggg gacgtgagtg tgacggacct gcaccctggg ggcactgcca 661 cctttcactg tgattcgggc taccagctgc agggagagga gaccctcatc tgcctcaatg 721 gcacccggcc atcctggaac ggtgaaaccc ccagctgcat ggcatcctgt ggtggcacca 781 tccacaatgc caccctgggc cgcatcgtgt ccccagagcc tgggggagcc gtagggccca 841 acctcacctg ccgttgggtc attgaagcag ctgaggggcg ccggctgcac ctgcactttg 901 aaagggtctc gctggatgag gacaatgacc ggctgatggt gcgctcaggg ggcagccccc 961 tatcccccgt gatctatgat tcggacatgg acgatgtccc cgagcggggt ctcatcagtg 1021 acgcccagtc cctctacgtg gagctgctgt cagagacacc tgccaatccc ctgctgttaa 1081 gccttcgatt tgaagccttt gaggaggatc gctgcttcgc ccccttcctg gcacatggaa 1141 atgtcactac cacggaccct gagtatcgcc caggggcact ggcaaccttc tcgtgcctcc 1201 caggatatgc cctggagccc cctgggcccc ccaatgccat cgaatgtgtg gatcccacag 1261 aaccccactg gaacgacaca gagccggcct gcaaagccat gtgtggaggg gagctgtcgg 1321 aaccagctgg cgtggtcctc tctcccgact ggccccagag ctatagcccg ggccaagact 1381 gcgtgtgggg cgtgcacgtc caggaagaga agcgcatctt gctccaagtt gagatattga 1441 atgtgcggga aggggacatg ctgacgctgt tcgacgggga cggtcccagc gcccgagtct 1501 tggcccagct gcggggacct cagccgcgcc gccgccttct ctcctctggg cccgacctca 1561 cactgcagtt tcaggcaccg cccgggcccc caaatccagg cctgggccag ggcttcgtat 1621 tgcacttcaa agaggtcccg aggaacgaca cgtgccccga gctgccacct ccggagtggg 1681 gctggagaac ggcatcccac ggggacctga tccggggcac ggtgctcacc taccagtgcg 1741 agcctggcta cgagctgcta ggctccgaca ttctcacttg ccagtgggac ctgtcttgga 1801 gcgccgcgcc gcccgcctgc caaaagatca tgacttgtgc tgaccctggc gagattgcca 1861 acgggcaccg caccgcctcg gacgccggct tccccgttgg ctcccacgtc cagtaccgct 1921 gcctgccagg gtacagcctc gagggggcag ccatgctcac ctgctacagc cgggacacag 1981 gcacacccaa gtggagcgat agggtcccca aatgcgcctt gaagtacgag ccgtgcctga 2041 acccgggggt tcccgagaat ggctaccaga cgctgtacaa gcaccactac caggcgggcg 2101 agtctcTGCG CTTCTTCTGC TATGAGGGCT TTGAGCTTAT CGGCGAGGTC ACCATCACCT 2161 GTGTGCCCGG CCACCCCTCC CAGTGGACCA GCCAGCCCCC ACTCTGCAAA GTTGCCTATG 2221 AGGAGCTCCT GGACAACCGA AAACTGGAAG TGACCCAGAC CACAGATCCA TCACGGCAGC 2281 TGGAAGGGGG GAACCTGGCC CTGGCCATCC TGCTGCCTCT AGGCTTGGTC ATTGTCCTCG 2341 GCAGTGGCGT TTACATCTAC TACACCAAGC TTCAGGGAAA GTCCCTTTTC GGCTTCTCGG 2401 GCTCCCACTC CTACAGCCCC ATCACCGTGG AGTCGGACTT CAGCAACCCG CTGTATGAAG 2461 CTGGGGATAC GCGGGAGTAT GAAGTTTCCA TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 2521 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 2581 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA 2641 TCTATATGCG ACAGGGGTCA GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 2701 ttgtgaaaga tt