Transcript: Human NM_001143974.2

Homo sapiens N-acylsphingosine amidohydrolase 2 (ASAH2), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-26
Taxon:
Homo sapiens (human)
Gene:
ASAH2 (56624)
Length:
4700
CDS:
1..2238

Additional Resources:

NCBI RefSeq record:
NM_001143974.2
NBCI Gene record:
ASAH2 (56624)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001143974.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000049515 GCTGGCACTATTGATGGAGTT pLKO.1 1267 CDS 100% 4.050 5.670 N ASAH2 n/a
2 TRCN0000049514 GCCTAGCATGTGCATTGCTAA pLKO.1 1140 CDS 100% 0.495 0.693 N ASAH2 n/a
3 TRCN0000421019 CAAAGATTTAGGAGGCCATTT pLKO_005 123 CDS 100% 10.800 7.560 N ASAH2 n/a
4 TRCN0000428597 CATCCTCACCAGGCTATACAG pLKO_005 396 CDS 100% 4.950 3.465 N ASAH2 n/a
5 TRCN0000049516 CCATCTTGTAAACAGTGACAA pLKO.1 933 CDS 100% 4.950 3.465 N ASAH2 n/a
6 TRCN0000049513 GCATCGACATAGGCATGGTAT pLKO.1 470 CDS 100% 4.950 3.465 N ASAH2 n/a
7 TRCN0000256527 GGAAGTTGCTGAAGTTATATT pLKO_005 1902 CDS 100% 15.000 7.500 Y ASAH2B n/a
8 TRCN0000256526 CAACAGTGGAATGGCATATTC pLKO_005 2087 CDS 100% 13.200 6.600 Y ASAH2B n/a
9 TRCN0000256524 GGACTCCTGGGTCTGAGTAAT pLKO_005 2065 CDS 100% 13.200 6.600 Y ASAH2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001143974.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12304 pDONR223 100% 97.3% 97.4% None 1_57del;189T>C;492A>C n/a
2 ccsbBroad304_12304 pLX_304 0% 97.3% 97.4% V5 1_57del;189T>C;492A>C n/a
3 TRCN0000468873 TCTACGGTGGGGTTCCCATGCCAT pLX_317 21.2% 97.3% 97.4% V5 1_57del;189T>C;492A>C n/a
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