Construct: ORF TRCN0000468873
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011042.1_s317c1
- Derived from:
- ccsbBroadEn_12304
- DNA Barcode:
- TCTACGGTGGGGTTCCCATGCCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ASAH2 (56624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468873
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56624 | ASAH2 | N-acylsphingosine amidohydr... | NM_001143974.2 | 97.3% | 97.4% | 1_57del;189T>C;492A>C |
| 2 | human | 56624 | ASAH2 | N-acylsphingosine amidohydr... | NM_019893.3 | 92.9% | 93% | (many diffs) |
| 3 | human | 56624 | ASAH2 | N-acylsphingosine amidohydr... | XM_011539970.2 | 92.9% | 93% | (many diffs) |
| 4 | human | 56624 | ASAH2 | N-acylsphingosine amidohydr... | XM_017016435.1 | 90.8% | 90% | (many diffs) |
| 5 | human | 56624 | ASAH2 | N-acylsphingosine amidohydr... | XM_011539971.2 | 86.7% | 86.7% | (many diffs) |
| 6 | human | 56624 | ASAH2 | N-acylsphingosine amidohydr... | XM_011539972.3 | 85.4% | 85.5% | (many diffs) |
| 7 | human | 56624 | ASAH2 | N-acylsphingosine amidohydr... | XM_011539973.3 | 74.1% | 72.9% | (many diffs) |
| 8 | human | 56624 | ASAH2 | N-acylsphingosine amidohydr... | XR_002956990.1 | 42% | (many diffs) | |
| 9 | human | 56624 | ASAH2 | N-acylsphingosine amidohydr... | XM_017016436.1 | 23.8% | 20% | 0_1ins1585;109_110ins74 |
| 10 | human | 653308 | ASAH2B | N-acylsphingosine amidohydr... | NM_001079516.4 | 22.6% | 18.5% | 0_1ins1609;33G>A;85_86ins74 |
| 11 | human | 653308 | ASAH2B | N-acylsphingosine amidohydr... | NM_001321957.2 | 22.6% | 18.5% | 0_1ins1609;33G>A;85_86ins74 |
| 12 | human | 653308 | ASAH2B | N-acylsphingosine amidohydr... | NM_001321958.2 | 21.9% | 21.9% | 0_1ins1698;2T>C |
| 13 | human | 653308 | ASAH2B | N-acylsphingosine amidohydr... | NM_001321959.2 | 21.9% | 21.9% | 0_1ins1698;2T>C |
| 14 | human | 653308 | ASAH2B | N-acylsphingosine amidohydr... | NM_001321960.2 | 21.9% | 21.9% | 0_1ins1698;2T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2247
- ORF length:
- 2178
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagtgccatc acagtggccc ttctcagcct cttgtttatc accagtggga 121 ccattgaaaa ccacaaagat ttaggaggcc attttttttc aaccacccaa agccctccag 181 ccacccaggg ctccacagcc gcccaacgct ccacagccac ccagcattcc acagccaccc 241 agagctccac agccactcaa acttctccag tgcctttaac cccagagtct cctctatttc 301 agaacttcag tggctaccat attggtgttg gacgagctga ctgcacagga caagtagcag 361 atatcaattt gatgggctat ggcaaatccg gccagaatgc acagggcatc ctcaccaggc 421 tatacagtcg tgccttcatc atggcagaac ctgatgggtc caatcgaaca gtgtttgtca 481 gcatcgacat aggcatggta tcccaaaggc tcaggctgga ggtcctgaac agactgcaga 541 gtaaatatgg ctccctgtac agaagagata atgtcatcct gagtggcact cacactcatt 601 caggtcctgc aggatatttc cagtataccg tgtttgtaat tgccagtgaa ggatttagca 661 atcaaacttt tcagcacatg gtcactggta tcttgaagag cattgacata gcacacacaa 721 atatgaaacc aggcaaaatc ttcatcaata aaggaaatgt ggatggtgtg cagatcaaca 781 gaagtccgta ttcttacctt caaaatccgc agtcagagag agcaaggtat tcttcaaata 841 cagacaagga aatgatagtt ttgaaaatgg tagatttgaa tggagatgac ttgggcctta 901 tcagctggtt tgccatccac ccggtcagca tgaacaacag taaccatctt gtaaacagtg 961 acaatgtggg ctatgcatct tacctgcttg agcaagagaa gaacaaagga tatctacctg 1021 gacaggggcc atttgtagca gcctttgctt catcaaacct aggagatgtg tcccccaaca 1081 ttcttggacc acgttgcatc aacacaggag agtcctgtga taacgccaat agcacttgtc 1141 ccattggtgg gcctagcatg tgcattgcta agggacctgg acaggatatg tttgacagca 1201 cacaaattat aggacgggcc atgtatcaga gagcaaagtc aaaaacatgt aaaccagcat 1261 tgggctacag ttttgcagct ggcactattg atggagttgg aggcctcaat tttacacagg 1321 ggaaaacaga aggggatcca ttttgggaca ccattcggga ccagatcctg ggaaagccat 1381 ctgaagaaat taaagaatgt cataaaccaa agcccatcct tcttcacacc ggagaactat 1441 caaaacctca cccctggcat ccagacattg ttgatgttca gattattacc cttgggtcct 1501 tggccataac tgccatcccc ggggagttta cgaccatgtc tggacgaaga cttcgagagg 1561 cagttcaagc agaatttgca tctcatggga tgcagaacat gactgttgtt atttcaggtc 1621 tatgcaacgt ctatacacat tacattacca cttatgaaga ataccaggct cagcgatatg 1681 aggcagcatc gacaatttat ggaccgcaca cattatctgc ttacattcag ctcttcagaa 1741 accttgctaa ggctattgct acggacacgg tagccaacct gagcagaggT CCAGAACCTC 1801 CCTTTTTCAA ACAATTAATA GTTCCATTAA TTCCTAGTAT TGTGGATAGA GCACCAAAAG 1861 GCAGAACTTT CGGGGATGTC CTGCAGCCAG CAAAACCTGA ATACAGAGTG GGGGAAGTTG 1921 CTGAAGTTAT ATTTGTAGGT GCTAACCCGA AGAATTCAGT ACAAAACCAG ACCCATCAGA 1981 CCTTCCTCAC TGTGGAGAAA TATGAGGCTA CTTCAACATC GTGGCAGATA GTGTGTAATG 2041 ATGCCTCCTG GGAGACTCGT TTTTATTGGC ACAAGGGACT CCTGGGTCTG AGTAATGCAA 2101 CAGTGGAATG GCATATTCCA GACACTGCCC AGCCTGGAAT CTACAGAATA AGATATTTTG 2161 GACACAATCG GAAGCAGGAC ATTCTGAAGC CTGCTGTCAT ACTTTCATTT GAAGGCACTT 2221 CCCCGGCTTT TGAAGTTGTA ACTATTTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 2281 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2341 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTACGGT 2401 GGGGTTCCCA TGCCATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2461 aagatt