Construct: ORF TRCN0000468873

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011042.1_s317c1
Derived from:
ccsbBroadEn_12304
DNA Barcode:
TCTACGGTGGGGTTCCCATGCCAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ASAH2 (56624)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468873
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56624 ASAH2 N-acylsphingosine amidohydr... NM_001143974.2 97.3% 97.4% 1_57del;189T>C;492A>C
2 human 56624 ASAH2 N-acylsphingosine amidohydr... NM_019893.3 92.9% 93% (many diffs)
3 human 56624 ASAH2 N-acylsphingosine amidohydr... XM_011539970.2 92.9% 93% (many diffs)
4 human 56624 ASAH2 N-acylsphingosine amidohydr... XM_017016435.1 90.8% 90% (many diffs)
5 human 56624 ASAH2 N-acylsphingosine amidohydr... XM_011539971.2 86.7% 86.7% (many diffs)
6 human 56624 ASAH2 N-acylsphingosine amidohydr... XM_011539972.3 85.4% 85.5% (many diffs)
7 human 56624 ASAH2 N-acylsphingosine amidohydr... XM_011539973.3 74.1% 72.9% (many diffs)
8 human 56624 ASAH2 N-acylsphingosine amidohydr... XR_002956990.1 42% (many diffs)
9 human 56624 ASAH2 N-acylsphingosine amidohydr... XM_017016436.1 23.8% 20% 0_1ins1585;109_110ins74
10 human 653308 ASAH2B N-acylsphingosine amidohydr... NM_001079516.4 22.6% 18.5% 0_1ins1609;33G>A;85_86ins74
11 human 653308 ASAH2B N-acylsphingosine amidohydr... NM_001321957.2 22.6% 18.5% 0_1ins1609;33G>A;85_86ins74
12 human 653308 ASAH2B N-acylsphingosine amidohydr... NM_001321958.2 21.9% 21.9% 0_1ins1698;2T>C
13 human 653308 ASAH2B N-acylsphingosine amidohydr... NM_001321959.2 21.9% 21.9% 0_1ins1698;2T>C
14 human 653308 ASAH2B N-acylsphingosine amidohydr... NM_001321960.2 21.9% 21.9% 0_1ins1698;2T>C
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2247
ORF length:
2178
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gagtgccatc acagtggccc ttctcagcct cttgtttatc accagtggga
121ccattgaaaa ccacaaagat ttaggaggcc attttttttc aaccacccaa agccctccag
181ccacccaggg ctccacagcc gcccaacgct ccacagccac ccagcattcc acagccaccc
241agagctccac agccactcaa acttctccag tgcctttaac cccagagtct cctctatttc
301agaacttcag tggctaccat attggtgttg gacgagctga ctgcacagga caagtagcag
361atatcaattt gatgggctat ggcaaatccg gccagaatgc acagggcatc ctcaccaggc
421tatacagtcg tgccttcatc atggcagaac ctgatgggtc caatcgaaca gtgtttgtca
481gcatcgacat aggcatggta tcccaaaggc tcaggctgga ggtcctgaac agactgcaga
541gtaaatatgg ctccctgtac agaagagata atgtcatcct gagtggcact cacactcatt
601caggtcctgc aggatatttc cagtataccg tgtttgtaat tgccagtgaa ggatttagca
661atcaaacttt tcagcacatg gtcactggta tcttgaagag cattgacata gcacacacaa
721atatgaaacc aggcaaaatc ttcatcaata aaggaaatgt ggatggtgtg cagatcaaca
781gaagtccgta ttcttacctt caaaatccgc agtcagagag agcaaggtat tcttcaaata
841cagacaagga aatgatagtt ttgaaaatgg tagatttgaa tggagatgac ttgggcctta
901tcagctggtt tgccatccac ccggtcagca tgaacaacag taaccatctt gtaaacagtg
961acaatgtggg ctatgcatct tacctgcttg agcaagagaa gaacaaagga tatctacctg
1021gacaggggcc atttgtagca gcctttgctt catcaaacct aggagatgtg tcccccaaca
1081ttcttggacc acgttgcatc aacacaggag agtcctgtga taacgccaat agcacttgtc
1141ccattggtgg gcctagcatg tgcattgcta agggacctgg acaggatatg tttgacagca
1201cacaaattat aggacgggcc atgtatcaga gagcaaagtc aaaaacatgt aaaccagcat
1261tgggctacag ttttgcagct ggcactattg atggagttgg aggcctcaat tttacacagg
1321ggaaaacaga aggggatcca ttttgggaca ccattcggga ccagatcctg ggaaagccat
1381ctgaagaaat taaagaatgt cataaaccaa agcccatcct tcttcacacc ggagaactat
1441caaaacctca cccctggcat ccagacattg ttgatgttca gattattacc cttgggtcct
1501tggccataac tgccatcccc ggggagttta cgaccatgtc tggacgaaga cttcgagagg
1561cagttcaagc agaatttgca tctcatggga tgcagaacat gactgttgtt atttcaggtc
1621tatgcaacgt ctatacacat tacattacca cttatgaaga ataccaggct cagcgatatg
1681aggcagcatc gacaatttat ggaccgcaca cattatctgc ttacattcag ctcttcagaa
1741accttgctaa ggctattgct acggacacgg tagccaacct gagcagaggT CCAGAACCTC
1801CCTTTTTCAA ACAATTAATA GTTCCATTAA TTCCTAGTAT TGTGGATAGA GCACCAAAAG
1861GCAGAACTTT CGGGGATGTC CTGCAGCCAG CAAAACCTGA ATACAGAGTG GGGGAAGTTG
1921CTGAAGTTAT ATTTGTAGGT GCTAACCCGA AGAATTCAGT ACAAAACCAG ACCCATCAGA
1981CCTTCCTCAC TGTGGAGAAA TATGAGGCTA CTTCAACATC GTGGCAGATA GTGTGTAATG
2041ATGCCTCCTG GGAGACTCGT TTTTATTGGC ACAAGGGACT CCTGGGTCTG AGTAATGCAA
2101CAGTGGAATG GCATATTCCA GACACTGCCC AGCCTGGAAT CTACAGAATA AGATATTTTG
2161GACACAATCG GAAGCAGGAC ATTCTGAAGC CTGCTGTCAT ACTTTCATTT GAAGGCACTT
2221CCCCGGCTTT TGAAGTTGTA ACTATTTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG
2281GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
2341AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATCTACGGT
2401GGGGTTCCCA TGCCATACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
2461aagatt

Download FASTA (ORF) (Full)