Transcript: Human NM_001184728.3

Homo sapiens phosphatidylinositol glycan anchor biosynthesis class T (PIGT), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
PIGT (51604)
Length:
2000
CDS:
17..1585

Additional Resources:

NCBI RefSeq record:
NM_001184728.3
NBCI Gene record:
PIGT (51604)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001184728.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000141139 CACGAGCCAAATGTGGCATTT pLKO.1 1740 3UTR 100% 10.800 15.120 N PIGT n/a
2 TRCN0000141070 CGGAAGACCTATGCCATCTAT pLKO.1 782 CDS 100% 5.625 7.875 N PIGT n/a
3 TRCN0000141797 GCAGACCCTGTCAGTTGTATT pLKO.1 565 CDS 100% 13.200 9.240 N PIGT n/a
4 TRCN0000280840 GCAGACCCTGTCAGTTGTATT pLKO_005 565 CDS 100% 13.200 9.240 N PIGT n/a
5 TRCN0000144421 CAAGGAGAACAAACCAAGTTA pLKO.1 1066 CDS 100% 5.625 3.938 N PIGT n/a
6 TRCN0000144499 CAAACCAAGTTACATCCACTA pLKO.1 1075 CDS 100% 4.050 2.835 N PIGT n/a
7 TRCN0000142286 GACCACTACTTTCTGCGCTAT pLKO.1 347 CDS 100% 4.050 2.835 N PIGT n/a
8 TRCN0000122663 GCAGAAATGCACGCTGTACTA pLKO.1 525 CDS 100% 4.950 2.970 N PIGT n/a
9 TRCN0000280768 GCAGAAATGCACGCTGTACTA pLKO_005 525 CDS 100% 4.950 2.970 N PIGT n/a
10 TRCN0000141515 CCTGTCATTCACACAAGGCTT pLKO.1 274 CDS 100% 2.640 1.584 N PIGT n/a
11 TRCN0000142054 GCTGTATTGGACAGCACAGAA pLKO.1 1850 3UTR 100% 0.495 0.297 N PIGT n/a
12 TRCN0000143693 GAGCCAAATGTGGCATTTGAA pLKO.1 1743 3UTR 100% 0.563 0.281 Y PIGT n/a
13 TRCN0000280842 GAGCCAAATGTGGCATTTGAA pLKO_005 1743 3UTR 100% 0.563 0.281 Y PIGT n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001184728.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08326 pDONR223 100% 90.1% 90.3% None 324_325ins168;1203G>A;1452T>C n/a
2 ccsbBroad304_08326 pLX_304 0% 90.1% 90.3% V5 324_325ins168;1203G>A;1452T>C n/a
3 TRCN0000476868 TGCGATGGTCCAAGTCACGTCTCG pLX_317 14.1% 90.1% 90.3% V5 324_325ins168;1203G>A;1452T>C n/a
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