Construct: ORF TRCN0000468300
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017880.1_s317c1
- Derived from:
- ccsbBroadEn_10749
- DNA Barcode:
- CCCGGTGGTGACCGCTCCGGTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATF2 (1386)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468300
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1386 | ATF2 | activating transcription fa... | NM_001256094.1 | 100% | 100% | |
| 2 | human | 1386 | ATF2 | activating transcription fa... | NM_001256090.2 | 41.3% | 41.3% | 627_1515delinsG |
| 3 | human | 1386 | ATF2 | activating transcription fa... | NM_001880.4 | 41.3% | 41.3% | 627_1515delinsG |
| 4 | human | 1386 | ATF2 | activating transcription fa... | NM_001256091.1 | 37.7% | 37.8% | 0_1ins54;573_1461delinsG |
| 5 | human | 1386 | ATF2 | activating transcription fa... | NM_001256093.2 | 34.8% | 33% | 0_1ins77;24_25ins97;453_1122delinsG |
| 6 | human | 1386 | ATF2 | activating transcription fa... | NM_001256092.2 | 29.8% | 28.2% | 0_1ins77;24_25ins97;453_1341delinsG |
| 7 | human | 1386 | ATF2 | activating transcription fa... | NR_045774.2 | 15.4% | 1_296del;923_4049delinsG | |
| 8 | human | 1386 | ATF2 | activating transcription fa... | NR_045771.2 | 14.7% | 1_296del;923_4238delinsG | |
| 9 | human | 1386 | ATF2 | activating transcription fa... | NR_045770.2 | 14.7% | 1_331del;958_4246delinsG | |
| 10 | human | 1386 | ATF2 | activating transcription fa... | NR_045768.2 | 14.3% | 1_331del;958_4361delinsG | |
| 11 | human | 1386 | ATF2 | activating transcription fa... | NR_045769.2 | 14.2% | 1_331del;958_4393delinsG | |
| 12 | human | 1386 | ATF2 | activating transcription fa... | NR_045773.2 | 12.4% | 1_296del;397_398ins97;826_4141delinsG | |
| 13 | human | 1386 | ATF2 | activating transcription fa... | NR_045772.2 | 12.3% | 1_331del;432_433ins97;861_4176delinsG | |
| 14 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284370.1 | 41.7% | 43.6% | (many diffs) |
| 15 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284371.1 | 41.7% | 43.6% | (many diffs) |
| 16 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284372.1 | 39.2% | 33.4% | (many diffs) |
| 17 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284373.1 | 39.2% | 33.4% | (many diffs) |
| 18 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001025093.2 | 36.2% | 37.8% | (many diffs) |
| 19 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284376.1 | 28.7% | 28.7% | (many diffs) |
| 20 | mouse | 11909 | Atf2 | activating transcription fa... | NM_001284369.1 | 28.3% | 28.3% | (many diffs) |
| 21 | mouse | 11909 | Atf2 | activating transcription fa... | NM_009715.3 | 28.3% | 28.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa attcaagtta catgtgaatt ctgccaggca atacaaggac ctgtggaata 121 tgagtgatga caaacccttt ctatgtactg cgcctggatg tggccagcgt tttaccaacg 181 aggatcattt ggctgtccat aaacataaac atgagatgac actgaaattt ggtccagcac 241 gtaatgacag tgtcattgtg gctgatcaga ccccaacacc aacaagattc ttgaaaaact 301 gtgaagaagt gggtttgttt aatgagttGG CGAGTCCATT TGAGAATGAA TTCAAGAAAG 361 CTTCAGAAGA TGACATTAAA AAAATGCCTC TAGATTTATC CCCTCTTGCA ACACCTATCA 421 TAAGAAGCAA AATTGAGGAG CCTTCTGTTG TAGAAACAAC TCACCAGGAT AGTCCTTTAC 481 CTCACCCAGA GTCTACTACC AGTGATGAGA AGGAAGTACC ATTGGCACAA ACTGCACAGC 541 CCACATCAGC TATTGTTCGT CCAGCATCAT TACAGGTTCC CAATGTGCTG CTTACAAGTT 601 CTGACTCAAG TGTAATTATT CAGCAGGCAG TACCTTCACC AACCTCAAGT ACTGTAATCA 661 CCCAGGCACC ATCCTCTAAC AGGCCAATTG TGTACCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 841 CGGTGGTGAC CGCTCCGGTC GAACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt