Transcript: Human NM_001257396.2

Homo sapiens ubiquitin conjugating enzyme E2 V1 (UBE2V1), transcript variant 8, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
UBE2V1 (7335)
Length:
1984
CDS:
23..340

Additional Resources:

NCBI RefSeq record:
NM_001257396.2
NBCI Gene record:
UBE2V1 (7335)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001257396.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000033707 AGGACAGTGTTACAGCAATTA pLKO.1 319 CDS 100% 13.200 6.600 Y UBE2V1 n/a
2 TRCN0000420546 CGATTTAATCAGTCTTCATTT pLKO_005 382 3UTR 100% 13.200 6.600 Y TMEM189-UBE2V1 n/a
3 TRCN0000422529 TAAATTGATTCCCATCATAAC pLKO_005 611 3UTR 100% 10.800 5.400 Y TMEM189-UBE2V1 n/a
4 TRCN0000033708 CGCCTAATGATGTCTAAAGAA pLKO.1 272 CDS 100% 5.625 2.813 Y UBE2V1 n/a
5 TRCN0000033704 CCCTGGTTTCTTTAAGTCTTA pLKO.1 854 3UTR 100% 4.950 2.475 Y UBE2V1 n/a
6 TRCN0000033706 CCAAGAGCCATATCAGTGCTA pLKO.1 200 CDS 100% 2.640 1.320 Y UBE2V1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001257396.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01741 pDONR223 100% 71.4% 71.4% None 171_172ins126 n/a
2 ccsbBroad304_01741 pLX_304 0% 71.4% 71.4% V5 171_172ins126 n/a
3 TRCN0000468843 CTGAGCTAAAGGCTACTCTTGCGC pLX_317 82.6% 71.4% 71.4% V5 171_172ins126 n/a
4 ccsbBroadEn_01742 pDONR223 100% 59% 65.3% None (many diffs) n/a
5 ccsbBroad304_01742 pLX_304 0% 59% 65.3% V5 (many diffs) n/a
6 TRCN0000474497 GGCACAGCCACCCTAATTTTCGTC pLX_317 87.6% 59% 65.3% V5 (many diffs) n/a
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