Construct: ORF TRCN0000474497
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004955.1_s317c1
- Derived from:
- ccsbBroadEn_01742
- DNA Barcode:
- GGCACAGCCACCCTAATTTTCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2V2 (7336)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474497
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7336 | UBE2V2 | ubiquitin conjugating enzym... | NM_003350.3 | 100% | 100% | |
| 2 | human | 7336 | UBE2V2 | ubiquitin conjugating enzym... | XM_017013808.2 | 97.4% | 95.8% | 4_4delCinsGCGGTCTCCA;7A>G |
| 3 | human | 7336 | UBE2V2 | ubiquitin conjugating enzym... | XM_011517583.3 | 83.4% | 80.9% | (many diffs) |
| 4 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001032288.3 | 83% | 89.1% | (many diffs) |
| 5 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257395.2 | 82.6% | 88.9% | (many diffs) |
| 6 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257393.2 | 71% | 75.2% | (many diffs) |
| 7 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_021988.6 | 71% | 75.2% | (many diffs) |
| 8 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_199144.3 | 71% | 75.2% | (many diffs) |
| 9 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257397.2 | 61.3% | 64.1% | (many diffs) |
| 10 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257398.1 | 61.3% | 64.1% | (many diffs) |
| 11 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257399.2 | 61.3% | 64.1% | (many diffs) |
| 12 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001282576.1 | 61.3% | 64.1% | (many diffs) |
| 13 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001282579.1 | 61.3% | 64.1% | (many diffs) |
| 14 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257396.2 | 59% | 65.3% | (many diffs) |
| 15 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001282580.1 | 35.1% | 38.7% | (many diffs) |
| 16 | human | 387522 | TMEM189-UBE2V1 | TMEM189-UBE2V1 readthrough | NM_199203.2 | 32.6% | 34.8% | (many diffs) |
| 17 | mouse | 70620 | Ube2v2 | ubiquitin-conjugating enzym... | NM_023585.4 | 95.1% | 98.6% | (many diffs) |
| 18 | mouse | 70620 | Ube2v2 | ubiquitin-conjugating enzym... | XM_011246011.2 | 68.5% | 71% | (many diffs) |
| 19 | mouse | 70620 | Ube2v2 | ubiquitin-conjugating enzym... | NM_001159351.1 | 67.8% | 70.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 501
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggtctccaca ggagttaaag ttcctcgtaa ttttcgcttg ttGGAAGAAC 121 TTGAAGAAGG ACAAAAAGGA GTAGGCGACG GTACAGTTAG CTGGGGCCTT GAAGATGATG 181 AAGATATGAC ACTTACAAGG TGGACAGGCA TGATTATTGG GCCACCAAGG ACAAATTATG 241 AAAACAGAAT ATATAGCCTG AAAGTAGAAT GTGGACCTAA ATACCCAGAA GCTCCTCCGT 301 CAGTTAGATT TGTAACAAAA ATTAATATGA ACGGAATAAA TAATTCCAGT GGGATGGTGG 361 ATGCCCGGAG CATACCAGTG TTAGCAAAAT GGCAAAATTC ATATAGCATT AAAGTTGTAC 421 TTCAAGAGCT AAGACGTCTA ATGATGTCCA AAGAAAATAT GAAGCTTCCA CAGCCACCAG 481 AAGGACAAAC ATACAACAAT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGCA CAGCCACCCT 661 AATTTTCGTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt