Construct: ORF TRCN0000468843
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005672.1_s317c1
- Derived from:
- ccsbBroadEn_01741
- DNA Barcode:
- CTGAGCTAAAGGCTACTCTTGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2V1 (7335)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468843
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001032288.3 | 100% | 100% | |
| 2 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257395.2 | 97.9% | 97.9% | 21_22insGGAGTAAAA |
| 3 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257393.2 | 84.7% | 82.3% | (many diffs) |
| 4 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_021988.6 | 84.7% | 82.3% | (many diffs) |
| 5 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_199144.3 | 84.7% | 82.3% | (many diffs) |
| 6 | human | 7336 | UBE2V2 | ubiquitin conjugating enzym... | NM_003350.3 | 83% | 89.1% | (many diffs) |
| 7 | human | 7336 | UBE2V2 | ubiquitin conjugating enzym... | XM_017013808.2 | 81.4% | 86.3% | (many diffs) |
| 8 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257396.2 | 71.4% | 71.4% | 171_172ins126 |
| 9 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257397.2 | 71.4% | 71.4% | 0_1ins126 |
| 10 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257398.1 | 71.4% | 71.4% | 0_1ins126 |
| 11 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257399.2 | 71.4% | 71.4% | 0_1ins126 |
| 12 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001282576.1 | 71.4% | 71.4% | 0_1ins126 |
| 13 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001282579.1 | 71.4% | 71.4% | 0_1ins126 |
| 14 | human | 7336 | UBE2V2 | ubiquitin conjugating enzym... | XM_011517583.3 | 71.4% | 73.9% | (many diffs) |
| 15 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001257394.2 | 67.4% | 63.9% | (many diffs) |
| 16 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_022442.6 | 67.4% | 63.9% | (many diffs) |
| 17 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001282575.3 | 61.4% | 61.2% | 0_1ins168;2_3delTGinsGA |
| 18 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001282577.3 | 61.4% | 61.2% | 0_1ins168;2_3delTGinsGA |
| 19 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001282578.3 | 61.4% | 61.2% | 0_1ins168;2_3delTGinsGA |
| 20 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NM_001282580.1 | 41.9% | 37.7% | (many diffs) |
| 21 | human | 387522 | TMEM189-UBE2V1 | TMEM189-UBE2V1 readthrough | NM_199203.2 | 39.2% | 38.1% | (many diffs) |
| 22 | human | 7335 | UBE2V1 | ubiquitin conjugating enzym... | NR_047554.2 | 14.3% | (many diffs) | |
| 23 | human | 107986871 | LOC107986871 | ubiquitin-conjugating enzym... | XR_002956669.1 | 7.4% | (many diffs) | |
| 24 | mouse | 66589 | Ube2v1 | ubiquitin-conjugating enzym... | NM_023230.2 | 87% | 93.8% | (many diffs) |
| 25 | mouse | 70620 | Ube2v2 | ubiquitin-conjugating enzym... | NM_023585.4 | 81.6% | 87.7% | (many diffs) |
| 26 | mouse | 66589 | Ube2v1 | ubiquitin-conjugating enzym... | NM_001311131.1 | 73% | 76.3% | (many diffs) |
| 27 | mouse | 66589 | Ube2v1 | ubiquitin-conjugating enzym... | NM_001311146.1 | 62.8% | 68.7% | (many diffs) |
| 28 | mouse | 70620 | Ube2v2 | ubiquitin-conjugating enzym... | XM_011246011.2 | 58.8% | 61.9% | (many diffs) |
| 29 | mouse | 70620 | Ube2v2 | ubiquitin-conjugating enzym... | NM_001159351.1 | 58.2% | 64.6% | (many diffs) |
| 30 | mouse | 66589 | Ube2v1 | ubiquitin-conjugating enzym... | XM_006500014.3 | 52.4% | 54.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agccaccacg ggctcgggag taaaagtccc tcgcaatttc cgactgttgg 121 aagaactcga agaaggCCAG AAAGGAGTAG GAGATGGCAC AGTTAGCTGG GGTCTAGAAG 181 ATGACGAAGA CATGACACTT ACAAGATGGA CAGGGATGAT AATTGGGCCT CCAAGAACAA 241 TTTATGAAAA CCGAATATAC AGCCTTAAAA TAGAATGTGG ACCTAAATAC CCAGAAGCAC 301 CCCCCTTTGT AAGATTTGTA ACAAAAATTA ATATGAATGG AGTAAATAGT TCTAATGGAG 361 TGGTGGACCC AAGAGCCATA TCAGTGCTAG CAAAATGGCA GAATTCATAT AGCATCAAAG 421 TTGTCCTGCA AGAGCTTCGG CGCCTAATGA TGTCTAAAGA AAATATGAAA CTCCCTCAGC 481 CGCCCGAAGG ACAGTGTTAC AGCAATTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTGAGCTAA 661 AGGCTACTCT TGCGCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 721 agatt