Transcript: Human NM_001278486.2

Homo sapiens anaphase promoting complex subunit 15 (ANAPC15), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
ANAPC15 (25906)
Length:
808
CDS:
137..514

Additional Resources:

NCBI RefSeq record:
NM_001278486.2
NBCI Gene record:
ANAPC15 (25906)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001278486.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000139846 CATCGCGGAGAAAGACAACAA pLKO.1 265 CDS 100% 4.950 6.930 N ANAPC15 n/a
2 TRCN0000143043 CTGTGGTTTAATCTGGATCGA pLKO.1 182 CDS 100% 2.640 3.696 N ANAPC15 n/a
3 TRCN0000140779 GACTACAATGAGTCACCGGAT pLKO.1 416 CDS 100% 2.160 1.728 N ANAPC15 n/a
4 TRCN0000344369 GACTACAATGAGTCACCGGAT pLKO_005 416 CDS 100% 2.160 1.728 N ANAPC15 n/a
5 TRCN0000121636 GACGAGATGAATGACTACAAT pLKO.1 404 CDS 100% 5.625 3.938 N ANAPC15 n/a
6 TRCN0000344429 GACGAGATGAATGACTACAAT pLKO_005 404 CDS 100% 5.625 3.938 N ANAPC15 n/a
7 TRCN0000139371 CAACCTGGTTCCTATTGGCAA pLKO.1 283 CDS 100% 2.640 1.848 N ANAPC15 n/a
8 TRCN0000143903 CATGGACGAGATGAATGACTA pLKO.1 400 CDS 100% 4.950 2.970 N ANAPC15 n/a
9 TRCN0000143366 GAGAAAGACAACAACCTGGTT pLKO.1 272 CDS 100% 2.640 1.584 N ANAPC15 n/a
10 TRCN0000344428 GAGAAAGACAACAACCTGGTT pLKO_005 272 CDS 100% 2.640 1.584 N ANAPC15 n/a
11 TRCN0000144934 GAAGAGGATGATGAAGATGAT pLKO.1 335 CDS 100% 4.950 2.475 Y ANAPC15 n/a
12 TRCN0000344368 GAAGAGGATGATGAAGATGAT pLKO_005 335 CDS 100% 4.950 2.475 Y ANAPC15 n/a
13 TRCN0000181505 GAGAAAGACAACAACCTGGTA pLKO.1 272 CDS 100% 2.640 1.584 N Anapc15 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001278486.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02882 pDONR223 100% 96.8% 96.8% None 317_328del n/a
2 ccsbBroad304_02882 pLX_304 0% 96.8% 96.8% V5 317_328del n/a
3 TRCN0000475223 CAATCCACTCCAGTTATACAATTA pLX_317 91.1% 96.8% 96.8% V5 317_328del n/a
4 ccsbBroadEn_07963 pDONR223 100% 96.5% 96.8% None 174C>T;317_328del n/a
5 ccsbBroad304_07963 pLX_304 0% 96.5% 96.8% V5 174C>T;317_328del n/a
6 TRCN0000467174 AGGCTCACCGTTGGTGCGTTGACA pLX_317 83.5% 96.5% 96.8% V5 174C>T;317_328del n/a
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