Transcript: Mouse NM_001282021.1

Mus musculus vasodilator-stimulated phosphoprotein (Vasp), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-06-03
Taxon:
Mus musculus (mouse)
Gene:
Vasp (22323)
Length:
2264
CDS:
337..1461

Additional Resources:

NCBI RefSeq record:
NM_001282021.1
NBCI Gene record:
Vasp (22323)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001282021.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000054419 CGGGCTACTGTGATGCTTTAT pLKO.1 364 CDS 100% 13.200 18.480 N Vasp n/a
2 TRCN0000054418 GCTAACTCCTTCCGAGTTGTT pLKO.1 466 CDS 100% 0.495 0.693 N Vasp n/a
3 TRCN0000328032 GGGCTACTGTGATGCTTTATG pLKO_005 365 CDS 100% 13.200 10.560 N Vasp n/a
4 TRCN0000327961 AGAGGAAATCATCGAAGTCTT pLKO_005 1407 CDS 100% 4.950 3.960 N Vasp n/a
5 TRCN0000353292 CCCATCGGAGGCAGCTATATA pLKO_005 1938 3UTR 100% 15.000 10.500 N Vasp n/a
6 TRCN0000327962 AGGAGGACGCCATACAGTTTG pLKO_005 623 CDS 100% 10.800 7.560 N Vasp n/a
7 TRCN0000054420 CCCAGAGTGAACCTGTGAGAA pLKO.1 1220 CDS 100% 4.950 3.465 N Vasp n/a
8 TRCN0000328033 GATCAGCAGGTGGTTATCAAC pLKO_005 505 CDS 100% 4.950 3.465 N Vasp n/a
9 TRCN0000054422 GCTGCCATTGCTGGAGCCAAA pLKO.1 1006 CDS 100% 1.350 0.945 N Vasp n/a
10 TRCN0000054421 CCATACAGTTTGCAACAGGCA pLKO.1 632 CDS 100% 0.660 0.462 N Vasp n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001282021.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11215 pDONR223 100% 86% 87.1% None (many diffs) n/a
2 ccsbBroad304_11215 pLX_304 0% 86% 87.1% V5 (many diffs) n/a
3 TRCN0000473358 GACTAAATGTCATTATTAATAAAT pLX_317 36.1% 86% 87.1% V5 (many diffs) n/a
4 ccsbBroadEn_13980 pDONR223 100% 85.9% 1% None (many diffs) n/a
5 ccsbBroad304_13980 pLX_304 0% 85.9% 1% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000472765 AGGGTTTGTAGTCCAAACGGGTGA pLX_317 8.1% 85.9% 1% V5 (not translated due to prior stop codon) (many diffs) n/a
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