Construct: ORF TRCN0000468540
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017718.1_s317c1
- Derived from:
- ccsbBroadEn_13426
- DNA Barcode:
- CAAGGGTATGTCCTGTGCAACCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF6 (221458)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468540
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221458 | KIF6 | kinesin family member 6 | NM_001289021.3 | 99.9% | 99.8% | 1987T>C |
| 2 | human | 221458 | KIF6 | kinesin family member 6 | NM_145027.6 | 92.7% | 91.7% | (many diffs) |
| 3 | human | 221458 | KIF6 | kinesin family member 6 | XM_011514358.3 | 92% | 91.1% | (many diffs) |
| 4 | human | 221458 | KIF6 | kinesin family member 6 | NM_001289020.3 | 90.6% | 89.6% | (many diffs) |
| 5 | human | 221458 | KIF6 | kinesin family member 6 | XM_011514359.3 | 90.6% | 89.6% | (many diffs) |
| 6 | human | 221458 | KIF6 | kinesin family member 6 | XM_005248904.4 | 89.1% | 88.2% | (many diffs) |
| 7 | human | 221458 | KIF6 | kinesin family member 6 | XM_011514357.3 | 87.1% | 86.2% | (many diffs) |
| 8 | human | 221458 | KIF6 | kinesin family member 6 | XM_017010427.1 | 76.9% | 69.3% | (many diffs) |
| 9 | human | 221458 | KIF6 | kinesin family member 6 | XR_001743238.1 | 68.3% | (many diffs) | |
| 10 | human | 221458 | KIF6 | kinesin family member 6 | XM_017010428.1 | 63.6% | 62.7% | (many diffs) |
| 11 | human | 221458 | KIF6 | kinesin family member 6 | XM_011514361.2 | 54.1% | 52.3% | (many diffs) |
| 12 | human | 221458 | KIF6 | kinesin family member 6 | NM_001289024.3 | 34.9% | 34.8% | 0_1ins1479;508T>C |
| 13 | human | 221458 | KIF6 | kinesin family member 6 | NM_001351566.2 | 33.3% | 33% | (many diffs) |
| 14 | human | 221458 | KIF6 | kinesin family member 6 | NM_001351503.1 | 14.1% | 12.3% | (many diffs) |
| 15 | mouse | 319991 | Kif6 | kinesin family member 6 | XM_006524391.3 | 74.7% | 77.1% | (many diffs) |
| 16 | mouse | 319991 | Kif6 | kinesin family member 6 | XM_006524393.3 | 57.4% | 60.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2343
- ORF length:
- 2274
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgaagcag actatccaga tattcgcgag ggtgaagccc cctgtccgga 121 agcaccaaca agggatttat tccatagatg aagatgaaaa attaatacct agcttggaaa 181 tcatcttacc acgtgatttg gcagatgggt ttgtgaataa taagcgagaa agctacaaat 241 ttaaatttca aagaattttt gatcaggatg caaaccaaga gaccgttttt gaaaacattg 301 ccaaaccagt tgctgggagt gtcctggcag gttacaatgg taccatcttt gcatatgggc 361 aaacaggcag cgggaagaca ttcactatca cagggggtgc agagcgttac agtgacagag 421 gcattatccc aaggacactg tcatacattt ttgaacagtt acaaaaggac agcagcaaaa 481 tatatacaac acacatttcc tatttggaaa tctacaatga atgtggttat gatcttttgg 541 atccaagaca tgaagcctcc agtttggaag atttgccgaa agtgacaata ctggaggatc 601 ctgatcagaa cattcacctg aaaaacttga ctctccatca ggcaaccaca gaggaagaag 661 ctctgaattt gcttttttta ggagacacca accgaatgat tgcagagact cctatgaacc 721 aagcttcaac ccgttcccac tgcattttca ccattcattt gtcaagcaag gaaccaggat 781 ctgcaactgt acgacatgcc aaactccatc tggttgacct ggctggttca gagcgagttg 841 caaagactgg agtagggggc catcttctaa cagaggccaa gtatatcaac ttgtcactac 901 attacttaga acaggttatc attgcccttt cagaaaagca ccgttcgcac attccttata 961 gaaactccat gatgaccagt gtcctaagag acagtttggg agggaactgc atgacaacta 1021 tgattgcaac actctccttg gagaaaagga atcttgatga gtctatatca acctgcagat 1081 ttgcacagcg agtggcactc ataaagaatg aagctgttct taatgaagaa attaacccca 1141 gattagtgat taaacgccta caaaaggaaa tccaggaact gaaggatgaa ctggccatgg 1201 tcactgggga gcagaggaca gaggcactca cagaagcaga gctccttcag ctggaaaaac 1261 taataacatc ctttttggaa gaccaggatt cagacagtag attagaggtt ggcgcggata 1321 tgcgtaaagt tcatcactgt tttcatcatt taaagaaact attgaatgac aagaagatcc 1381 ttgaaaacaa tacagtctcc tctgaaagca aagaccaaga ttgtcaagaa ccattaaaag 1441 aagaagaata tagaaagcta cgagatattc tgaaacagag agataacgaa atcagtatcc 1501 tttctgaagt gatgaagaaa cttcctctat cctggtttga aaggggaatg agagaggaaa 1561 tgtcattagg atgccaggag gcttttgaaa tcttcaagag ggaccacgct gacagcgtta 1621 ccatcgatga caacaaacag attctgaaac agagattttc tgaagccaag gccctgggag 1681 aaagtataaa tgaagcaaga agtaaaattg gtcacctgaa ggaagaaatc acccagcggc 1741 atatacagca agtagcccta ggaatctcgg aaaacatggc cgtgcctctg atgccagacc 1801 agcaggagga gaagctgcga tcacaactgg aggaagaaaa gagaaggtat aaaacaatgt 1861 tcactcgcct gaaagccctg aaggtggaga tcgagcactt gcagctgctc atggacaaag 1921 ccaaggtgaa gctacagaaa gagttTGAAG TCTGGTGGGC AGAGGAGGCC ACCAACCTGC 1981 AGGTAAATTC TCCAGCAGTG AATTCACTCG ATCACACGAA GCCATTTCTC CAGACATCTG 2041 ACTCCCAGCA TGAACGGTCC CAACTCCTCT CTAACAAAAG TTCTGGAGGC TGGGAAGTCC 2101 AAGATCAAGG CACTGGCAGA TTCGATGTCT GTGATGTGAA TGCCAGGAAA ATCCTGCCCT 2161 CGCCTTGCCC CAGTCCACAC AGCCAGAAAC AGAGCAGCAC CAGCACCCCA CTGGAAGACA 2221 GCATCCCCAA GAGGCCAGTG TCGTCCATCC CTCTCACCGG AGACAGCCAG ACGGACTCGG 2281 ACATCATCGC CTTCATCAAG GCCAGACAGA GCATTCTGCA GAAGCAATGT TTGGGAAGCA 2341 ATTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2401 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2461 GGCTTTATAT ATCTTGTGGA AAGGACGACA AGGGTATGTC CTGTGCAACC AAACGCGTTA 2521 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt