Construct: ORF ccsbBroad304_13426

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017718.1_s304c1, BRDN0000401515
Derived from:
ccsbBroadEn_13426
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KIF6 (221458)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13426
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 221458 KIF6 kinesin family member 6 NM_001289021.3 99.9% 99.8% 1987T>C
2 human 221458 KIF6 kinesin family member 6 NM_145027.6 92.7% 91.7% (many diffs)
3 human 221458 KIF6 kinesin family member 6 XM_011514358.3 92% 91.1% (many diffs)
4 human 221458 KIF6 kinesin family member 6 NM_001289020.3 90.6% 89.6% (many diffs)
5 human 221458 KIF6 kinesin family member 6 XM_011514359.3 90.6% 89.6% (many diffs)
6 human 221458 KIF6 kinesin family member 6 XM_005248904.4 89.1% 88.2% (many diffs)
7 human 221458 KIF6 kinesin family member 6 XM_011514357.3 87.1% 86.2% (many diffs)
8 human 221458 KIF6 kinesin family member 6 XM_017010427.1 76.9% 69.3% (many diffs)
9 human 221458 KIF6 kinesin family member 6 XR_001743238.1 68.3% (many diffs)
10 human 221458 KIF6 kinesin family member 6 XM_017010428.1 63.6% 62.7% (many diffs)
11 human 221458 KIF6 kinesin family member 6 XM_011514361.2 54.1% 52.3% (many diffs)
12 human 221458 KIF6 kinesin family member 6 NM_001289024.3 34.9% 34.8% 0_1ins1479;508T>C
13 human 221458 KIF6 kinesin family member 6 NM_001351566.2 33.3% 33% (many diffs)
14 human 221458 KIF6 kinesin family member 6 NM_001351503.1 14.1% 12.3% (many diffs)
15 mouse 319991 Kif6 kinesin family member 6 XM_006524391.3 74.7% 77.1% (many diffs)
16 mouse 319991 Kif6 kinesin family member 6 XM_006524393.3 57.4% 60.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2343
ORF length:
2274
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggtgaagcag actatccaga tattcgcgag ggtgaagccc cctgtccgga
121agcaccaaca agggatttat tccatagatg aagatgaaaa attaatacct agcttggaaa
181tcatcttacc acgtgatttg gcagatgggt ttgtgaataa taagcgagaa agctacaaat
241ttaaatttca aagaattttt gatcaggatg caaaccaaga gaccgttttt gaaaacattg
301ccaaaccagt tgctgggagt gtcctggcag gttacaatgg taccatcttt gcatatgggc
361aaacaggcag cgggaagaca ttcactatca cagggggtgc agagcgttac agtgacagag
421gcattatccc aaggacactg tcatacattt ttgaacagtt acaaaaggac agcagcaaaa
481tatatacaac acacatttcc tatttggaaa tctacaatga atgtggttat gatcttttgg
541atccaagaca tgaagcctcc agtttggaag atttgccgaa agtgacaata ctggaggatc
601ctgatcagaa cattcacctg aaaaacttga ctctccatca ggcaaccaca gaggaagaag
661ctctgaattt gcttttttta ggagacacca accgaatgat tgcagagact cctatgaacc
721aagcttcaac ccgttcccac tgcattttca ccattcattt gtcaagcaag gaaccaggat
781ctgcaactgt acgacatgcc aaactccatc tggttgacct ggctggttca gagcgagttg
841caaagactgg agtagggggc catcttctaa cagaggccaa gtatatcaac ttgtcactac
901attacttaga acaggttatc attgcccttt cagaaaagca ccgttcgcac attccttata
961gaaactccat gatgaccagt gtcctaagag acagtttggg agggaactgc atgacaacta
1021tgattgcaac actctccttg gagaaaagga atcttgatga gtctatatca acctgcagat
1081ttgcacagcg agtggcactc ataaagaatg aagctgttct taatgaagaa attaacccca
1141gattagtgat taaacgccta caaaaggaaa tccaggaact gaaggatgaa ctggccatgg
1201tcactgggga gcagaggaca gaggcactca cagaagcaga gctccttcag ctggaaaaac
1261taataacatc ctttttggaa gaccaggatt cagacagtag attagaggtt ggcgcggata
1321tgcgtaaagt tcatcactgt tttcatcatt taaagaaact attgaatgac aagaagatcc
1381ttgaaaacaa tacagtctcc tctgaaagca aagaccaaga ttgtcaagaa ccattaaaag
1441aagaagaata tagaaagcta cgagatattc tgaaacagag agataacgaa atcagtatcc
1501tttctgaagt gatgaagaaa cttcctctat cctggtttga aaggggaatg agagaggaaa
1561tgtcattagg atgccaggag gcttttgaaa tcttcaagag ggaccacgct gacagcgtta
1621ccatcgatga caacaaacag attctgaaac agagattttc tgaagccaag gccctgggag
1681aaagtataaa tgaagcaaga agtaaaattg gtcacctgaa ggaagaaatc acccagcggc
1741atatacagca agtagcccta ggaatctcgg aaaacatggc cgtgcctctg atgccagacc
1801agcaggagga gaagctgcga tcacaactgg aggaagaaaa gagaaggtat aaaacaatgt
1861tcactcgcct gaaagccctg aaggtggaga tcgagcactt gcagctgctc atggacaaag
1921ccaaggtgaa gctacagaaa gagtttgaag tctggtgggc agaggaggcc accaacctgc
1981aggtaaattc tccagcagtg aattcactcg atcacacgaa gccatttctc cagacatctg
2041actcccagca tgaacggtcc caactcctct ctaacaaaag ttctggaggc tgggaagtcc
2101aagatcaagg cactggcaga ttcgatgtct gtgatgtgaa tgccaggaaa atcctgccct
2161cgccttgccc cagtccacac agccagaaac agagcagcac cagcacccca ctggaagaca
2221gcatccccaa gaggccagtg tcgtccatcc ctctcaccgg agacagccag acggactcgg
2281acatcatcgc cttcatcaag gccagacaga gcattctgca gaagcaatgt ttgggaagca
2341atttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
2401tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
2461tctggatta

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