Transcript: Human NM_001308382.1

Homo sapiens DNA polymerase lambda (POLL), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-08-02
Taxon:
Homo sapiens (human)
Gene:
POLL (27343)
Length:
1644
CDS:
163..1065

Additional Resources:

NCBI RefSeq record:
NM_001308382.1
NBCI Gene record:
POLL (27343)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001308382.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000438695 TGGTGCCCTATAGCGAGTTTG pLKO_005 815 CDS 100% 10.800 15.120 N POLL n/a
2 TRCN0000425960 TGTGTGGCATGTGGTTCATAC pLKO_005 571 CDS 100% 10.800 7.560 N POLL n/a
3 TRCN0000437521 TGCGGAAGCTGGACCATATCA pLKO_005 302 CDS 100% 5.625 3.938 N POLL n/a
4 TRCN0000053129 CCTGAAGCATTACAGTGACTT pLKO.1 465 CDS 100% 4.950 3.465 N POLL n/a
5 TRCN0000447193 TGGTGATGTCGACGTGCTCAT pLKO_005 612 CDS 100% 4.050 2.835 N POLL n/a
6 TRCN0000053131 CAAGAGGAGAATGGTCAGCAA pLKO.1 727 CDS 100% 2.640 1.848 N POLL n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001308382.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11865 pDONR223 100% 99.3% 99.3% None 1_6delATGCTG n/a
2 ccsbBroad304_11865 pLX_304 0% 99.3% 99.3% V5 1_6delATGCTG n/a
3 TRCN0000468471 CACCTACGGTCTGTGGGAAGGCCA pLX_317 40.1% 99.3% 99.3% V5 1_6delATGCTG n/a
Download CSV