Construct: ORF ccsbBroad304_11865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015972.1_s304c1, BRDN0000409944
- Derived from:
- ccsbBroadEn_11865
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLL (27343)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11865
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27343 | POLL | DNA polymerase lambda | NM_001308382.1 | 99.3% | 99.3% | 1_6delATGCTG |
| 2 | human | 27343 | POLL | DNA polymerase lambda | XM_006717777.2 | 99.3% | 99.3% | 1_6delATGCTG |
| 3 | human | 27343 | POLL | DNA polymerase lambda | XM_011539664.2 | 97% | 97% | 1_6delATGCTG;537_557del |
| 4 | human | 27343 | POLL | DNA polymerase lambda | XM_011539665.2 | 97% | 97% | 1_6delATGCTG;537_557del |
| 5 | human | 27343 | POLL | DNA polymerase lambda | XM_011539666.1 | 97% | 97% | 1_6delATGCTG;537_557del |
| 6 | human | 27343 | POLL | DNA polymerase lambda | XM_017016093.1 | 97% | 97% | 1_6delATGCTG;537_557del |
| 7 | human | 27343 | POLL | DNA polymerase lambda | XM_006717775.2 | 94.9% | 94.9% | 1_6delATGCTG;65_106del |
| 8 | human | 27343 | POLL | DNA polymerase lambda | XM_006717776.1 | 94.9% | 94.9% | 1_6delATGCTG;65_106del |
| 9 | human | 27343 | POLL | DNA polymerase lambda | XM_011539662.2 | 92.8% | 92.8% | 1_6delATGCTG;65_106del;579_599del |
| 10 | human | 27343 | POLL | DNA polymerase lambda | XM_011539663.1 | 92.8% | 92.8% | 1_6delATGCTG;65_106del;579_599del |
| 11 | human | 27343 | POLL | DNA polymerase lambda | XM_011539667.1 | 89.5% | 89.5% | 0_1ins93 |
| 12 | human | 27343 | POLL | DNA polymerase lambda | XM_024447945.1 | 85.1% | 83.6% | (many diffs) |
| 13 | human | 27343 | POLL | DNA polymerase lambda | XM_024447944.1 | 83.4% | 81.9% | (many diffs) |
| 14 | human | 27343 | POLL | DNA polymerase lambda | XM_017016091.2 | 73.3% | 73.3% | 1_6delATGCTG;65_382del |
| 15 | human | 27343 | POLL | DNA polymerase lambda | XM_011539660.1 | 72.1% | 72.1% | 1_6delATGCTG;65_382del;855_875del |
| 16 | human | 27343 | POLL | DNA polymerase lambda | XM_017016089.1 | 72.1% | 72.1% | 1_6delATGCTG;65_382del;855_875del |
| 17 | human | 27343 | POLL | DNA polymerase lambda | XM_017016087.1 | 65.4% | 53.1% | (many diffs) |
| 18 | human | 27343 | POLL | DNA polymerase lambda | XM_017016086.2 | 62.6% | 60.3% | (many diffs) |
| 19 | human | 27343 | POLL | DNA polymerase lambda | XM_011539656.1 | 61.7% | 59.4% | (many diffs) |
| 20 | human | 27343 | POLL | DNA polymerase lambda | NM_001174085.1 | 61.1% | 58.3% | (many diffs) |
| 21 | human | 27343 | POLL | DNA polymerase lambda | XM_011539655.1 | 61.1% | 58.3% | (many diffs) |
| 22 | human | 27343 | POLL | DNA polymerase lambda | XM_011539654.1 | 60.2% | 57.5% | (many diffs) |
| 23 | human | 27343 | POLL | DNA polymerase lambda | XM_024447943.1 | 60% | 56.2% | (many diffs) |
| 24 | human | 27343 | POLL | DNA polymerase lambda | XM_011539657.1 | 59.2% | 55.4% | (many diffs) |
| 25 | human | 27343 | POLL | DNA polymerase lambda | XM_024447942.1 | 59.2% | 55.4% | (many diffs) |
| 26 | human | 27343 | POLL | DNA polymerase lambda | XM_017016085.1 | 56.8% | 37.6% | (many diffs) |
| 27 | human | 27343 | POLL | DNA polymerase lambda | XM_017016084.1 | 56% | 37.1% | (many diffs) |
| 28 | human | 27343 | POLL | DNA polymerase lambda | NM_001174084.2 | 50.8% | 47.7% | (many diffs) |
| 29 | human | 27343 | POLL | DNA polymerase lambda | NM_013274.3 | 50.8% | 47.7% | (many diffs) |
| 30 | human | 27343 | POLL | DNA polymerase lambda | XM_011539651.1 | 50.8% | 47.7% | (many diffs) |
| 31 | human | 27343 | POLL | DNA polymerase lambda | XM_011539650.1 | 50.2% | 47.2% | (many diffs) |
| 32 | human | 27343 | POLL | DNA polymerase lambda | XM_011539652.2 | 50.2% | 47.2% | (many diffs) |
| 33 | human | 27343 | POLL | DNA polymerase lambda | XM_011539653.1 | 50.2% | 47.2% | (many diffs) |
| 34 | human | 27343 | POLL | DNA polymerase lambda | NR_033406.1 | 37.5% | (many diffs) | |
| 35 | mouse | 56626 | Poll | polymerase (DNA directed), ... | NM_001330506.1 | 44.7% | 42.9% | (many diffs) |
| 36 | mouse | 56626 | Poll | polymerase (DNA directed), ... | NM_020032.3 | 44.7% | 42.9% | (many diffs) |
| 37 | mouse | 56626 | Poll | polymerase (DNA directed), ... | XM_011247301.2 | 44.7% | 42.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca tcatcaaaag tacttgcaaa gattcctagg agggaagagg gagaagaagc 121 agaaggaggc ctgcagtatc cctgggattg ggaagcggat ggctgagaaa atcatagaga 181 tcctggagag cgggcatttg cggaagctgg accatatcag tgagagcgtg cctgtcttgg 241 agctcttctc caacatctgg ggagctggga ccaagactgc ccagatgtgg taccaacagg 301 gcttccgaag tctggaagac atccgcagcc aggcctccct gacaacccag caggccatcg 361 gcctgaagca ttacagtgac ttcctggaac gtatgcccag ggaggaggct acagagattg 421 agcagacagt ccagaaagca gcccaggcct ttaactctgg gctgctgtgt gtggcatgtg 481 gttcataccg acggggaaag gcgacctgtg gtgatgtcga cgtgctcatc actcacccag 541 atggccggtc ccaccggggt atcttcagcc gcctccttga cagtcttcgg caggaagggt 601 tcctcacaga tgacttggtg agccaagagg agaatggtca gcaacagaag tacttggggg 661 tgtgccggct cccagggcca gggcggcggc accggcgcct ggacatcatc gtggtgccct 721 atagcgagtt tgcctgtgcc ctgctctact tcaccggctc tgcacacttc aaccgctcca 781 tgcgagccct ggccaaaacc aagggcatga gtctgtcaga acatgccctc agcactgctg 841 tggtccggaa cacccatggc tgcaaggtgg ggcctggccg agtgctgccc actcccactg 901 agaaggatgt cttcaggctc ttaggcctcc cctaccgaga acctgctgag cgggactggt 961 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1021 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1081 ggatta