Construct: ORF TRCN0000468471

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF015972.1_s317c1
• Derived from: ccsbBroadEn_11865
• DNA Barcode: CACCTACGGTCTGTGGGAAGGCCA
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: POLL (27343)

Vector Information:

• Vector Backbone: pLX_317
• Pol II Cassette 1: SV40-PuroR
• Pol II Cassette 2: EF1a-TRCN0000468471
• Selection Marker: PuroR
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	27343	POLL	DNA polymerase lambda	NM_001308382.1	99.3%	99.3%	1_6delATGCTG
2	human	27343	POLL	DNA polymerase lambda	XM_006717777.2	99.3%	99.3%	1_6delATGCTG
3	human	27343	POLL	DNA polymerase lambda	XM_011539664.2	97%	97%	1_6delATGCTG;537_557del
4	human	27343	POLL	DNA polymerase lambda	XM_011539665.2	97%	97%	1_6delATGCTG;537_557del
5	human	27343	POLL	DNA polymerase lambda	XM_011539666.1	97%	97%	1_6delATGCTG;537_557del
6	human	27343	POLL	DNA polymerase lambda	XM_017016093.1	97%	97%	1_6delATGCTG;537_557del
7	human	27343	POLL	DNA polymerase lambda	XM_006717775.2	94.9%	94.9%	1_6delATGCTG;65_106del
8	human	27343	POLL	DNA polymerase lambda	XM_006717776.1	94.9%	94.9%	1_6delATGCTG;65_106del
9	human	27343	POLL	DNA polymerase lambda	XM_011539662.2	92.8%	92.8%	1_6delATGCTG;65_106del;579_599del
10	human	27343	POLL	DNA polymerase lambda	XM_011539663.1	92.8%	92.8%	1_6delATGCTG;65_106del;579_599del
11	human	27343	POLL	DNA polymerase lambda	XM_011539667.1	89.5%	89.5%	0_1ins93
12	human	27343	POLL	DNA polymerase lambda	XM_024447945.1	85.1%	83.6%	(many diffs)
13	human	27343	POLL	DNA polymerase lambda	XM_024447944.1	83.4%	81.9%	(many diffs)
14	human	27343	POLL	DNA polymerase lambda	XM_017016091.2	73.3%	73.3%	1_6delATGCTG;65_382del
15	human	27343	POLL	DNA polymerase lambda	XM_011539660.1	72.1%	72.1%	1_6delATGCTG;65_382del;855_875del
16	human	27343	POLL	DNA polymerase lambda	XM_017016089.1	72.1%	72.1%	1_6delATGCTG;65_382del;855_875del
17	human	27343	POLL	DNA polymerase lambda	XM_017016087.1	65.4%	53.1%	(many diffs)
18	human	27343	POLL	DNA polymerase lambda	XM_017016086.2	62.6%	60.3%	(many diffs)
19	human	27343	POLL	DNA polymerase lambda	XM_011539656.1	61.7%	59.4%	(many diffs)
20	human	27343	POLL	DNA polymerase lambda	NM_001174085.1	61.1%	58.3%	(many diffs)
21	human	27343	POLL	DNA polymerase lambda	XM_011539655.1	61.1%	58.3%	(many diffs)
22	human	27343	POLL	DNA polymerase lambda	XM_011539654.1	60.2%	57.5%	(many diffs)
23	human	27343	POLL	DNA polymerase lambda	XM_024447943.1	60%	56.2%	(many diffs)
24	human	27343	POLL	DNA polymerase lambda	XM_011539657.1	59.2%	55.4%	(many diffs)
25	human	27343	POLL	DNA polymerase lambda	XM_024447942.1	59.2%	55.4%	(many diffs)
26	human	27343	POLL	DNA polymerase lambda	XM_017016085.1	56.8%	37.6%	(many diffs)
27	human	27343	POLL	DNA polymerase lambda	XM_017016084.1	56%	37.1%	(many diffs)
28	human	27343	POLL	DNA polymerase lambda	NM_001174084.2	50.8%	47.7%	(many diffs)
29	human	27343	POLL	DNA polymerase lambda	NM_013274.3	50.8%	47.7%	(many diffs)
30	human	27343	POLL	DNA polymerase lambda	XM_011539651.1	50.8%	47.7%	(many diffs)
31	human	27343	POLL	DNA polymerase lambda	XM_011539650.1	50.2%	47.2%	(many diffs)
32	human	27343	POLL	DNA polymerase lambda	XM_011539652.2	50.2%	47.2%	(many diffs)
33	human	27343	POLL	DNA polymerase lambda	XM_011539653.1	50.2%	47.2%	(many diffs)
34	human	27343	POLL	DNA polymerase lambda	NR_033406.1	37.5%		(many diffs)
35	mouse	56626	Poll	polymerase (DNA directed), ...	NM_001330506.1	44.7%	42.9%	(many diffs)
36	mouse	56626	Poll	polymerase (DNA directed), ...	NM_020032.3	44.7%	42.9%	(many diffs)
37	mouse	56626	Poll	polymerase (DNA directed), ...	XM_011247301.2	44.7%	42.9%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

960

ORF length:

894

Sequence:

1	tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61	ttggcatgca tcatcaaaag tacttgcaaa gattcctagg agggaagagg gagaagaagc
121	agaaggaggc ctgcagtatc cctgggattg ggaagcggat ggctgagaaa atcatagaga
181	tcctggagag cgggcatttg cggaagctgg accatatcag tgagagcgtg cctgtcttgg
241	agctcttctc caacatctgg ggagctggga ccaagactgc ccagatgtgg taccaacagg
301	gcttccgaag tctggaagac atccgcagcc aggcctccct gacaacccag caggccatcg
361	gcctgaagca ttacagtgac ttcctggaac gtatgcccag ggaggaggct acagagattg
421	agcagacagt ccagaaagca gcccaggcct ttaactctgg gctgctgtgt gtggcatgtg
481	gttcataccg acggggaaag gcgacctgtg gtgatgtcga cgtgctcatc actcacccag
541	atggccggtc ccaccggggt atcttcagcc gcctccttga cagtcttcgg caggaagggt
601	tccTCACAGA TGACTTGGTG AGCCAAGAGG AGAATGGTCA GCAACAGAAG TACTTGGGGG
661	TGTGCCGGCT CCCAGGGCCA GGGCGGCGGC ACCGGCGCCT GGACATCATC GTGGTGCCCT
721	ATAGCGAGTT TGCCTGTGCC CTGCTCTACT TCACCGGCTC TGCACACTTC AACCGCTCCA
781	TGCGAGCCCT GGCCAAAACC AAGGGCATGA GTCTGTCAGA ACATGCCCTC AGCACTGCTG
841	TGGTCCGGAA CACCCATGGC TGCAAGGTGG GGCCTGGCCG AGTGCTGCCC ACTCCCACTG
901	AGAAGGATGT CTTCAGGCTC TTAGGCCTCC CCTACCGAGA ACCTGCTGAG CGGGACTGGT
961	GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
1021	GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
1081	TTTATATATC TTGTGGAAAG GACGACACCT ACGGTCTGTG GGAAGGCCAA CGCGTTAAGT
1141	Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

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