Construct: ORF ccsbBroad304_11852

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF009159.1_s304c1, BRDN0000399879
• Derived from: ccsbBroadEn_11852
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: AGO2 (27161)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_11852
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	27161	AGO2	argonaute RISC catalytic co...	XM_017013317.2	48.2%	48.2%	1_1215del
2	human	192669	AGO3	argonaute RISC catalytic co...	XM_005270576.4	48.1%	58.4%	(many diffs)
3	human	192669	AGO3	argonaute RISC catalytic co...	XM_017000528.2	48.1%	58.4%	(many diffs)
4	human	27161	AGO2	argonaute RISC catalytic co...	XM_011516966.3	46.3%	46.3%	1_1308del
5	human	192669	AGO3	argonaute RISC catalytic co...	NM_177422.3	44.9%	54.6%	(many diffs)
6	human	192669	AGO3	argonaute RISC catalytic co...	XM_011540882.3	44.9%	54.6%	(many diffs)
7	human	192669	AGO3	argonaute RISC catalytic co...	XM_017000524.2	44.9%	54.6%	(many diffs)
8	human	192669	AGO3	argonaute RISC catalytic co...	XM_017000525.2	44.9%	54.6%	(many diffs)
9	human	192669	AGO3	argonaute RISC catalytic co...	XM_017000526.2	44.9%	54.6%	(many diffs)
10	human	192669	AGO3	argonaute RISC catalytic co...	XM_017000527.2	44.9%	54.6%	(many diffs)
11	human	27161	AGO2	argonaute RISC catalytic co...	NM_012154.5	43.8%	43.8%	1_1446del
12	human	27161	AGO2	argonaute RISC catalytic co...	XM_011516965.2	43.1%	43.1%	1_1488del
13	human	26523	AGO1	argonaute RISC component 1	XM_017001019.1	42.7%	47.8%	(many diffs)
14	human	27161	AGO2	argonaute RISC catalytic co...	XM_011516968.2	41%	41%	1_1623del
15	human	27161	AGO2	argonaute RISC catalytic co...	NM_001164623.3	39.9%	39.9%	1_1446del;2168_2169ins102
16	human	26523	AGO1	argonaute RISC component 1	NM_001317123.1	38.5%	43.2%	(many diffs)
17	human	26523	AGO1	argonaute RISC component 1	NM_012199.5	35.2%	39.4%	(many diffs)
18	human	26523	AGO1	argonaute RISC component 1	XM_011541236.2	35.1%	39.3%	(many diffs)
19	human	192669	AGO3	argonaute RISC catalytic co...	XM_011540879.3	33.3%	40.4%	(many diffs)
20	human	192669	AGO3	argonaute RISC catalytic co...	NM_024852.4	32.7%	39.7%	(many diffs)
21	human	192669	AGO3	argonaute RISC catalytic co...	XM_005270575.4	32.7%	39.7%	(many diffs)
22	human	192669	AGO3	argonaute RISC catalytic co...	XM_017000523.2	32.7%	39.7%	(many diffs)
23	mouse	236511	Ago1	argonaute RISC catalytic su...	NM_001317174.1	43%	47.8%	(many diffs)
24	mouse	236511	Ago1	argonaute RISC catalytic su...	XM_011240521.1	42.9%	47.6%	(many diffs)
25	mouse	239528	Ago2	argonaute RISC catalytic su...	NM_153178.4	39.1%	43.8%	(many diffs)
26	mouse	236511	Ago1	argonaute RISC catalytic su...	NM_153403.3	35.5%	39.4%	(many diffs)
27	mouse	236511	Ago1	argonaute RISC catalytic su...	XM_011240520.2	35.3%	39.3%	(many diffs)
28	mouse	236511	Ago1	argonaute RISC catalytic su...	NM_001317173.1	34%	39.4%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

1197

ORF length:

1131

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatgcc catccagggc cagccgtgct tctgcaaata cgcgcagggg gcggacagcg
121	tggagcccat gttccggcac ctgaagaaca cgtatgcggg cctgcagctg gtggtggtca
181	tcctgcccgg caagacgccc gtgtacgccg aggtcaagcg cgtgggagac acggtgctgg
241	ggatggccac gcagtgcgtg cagatgaaga acgtgcagag gaccacgcca cagaccctgt
301	ccaacctctg cctgaagatc aacgtcaagc tgggaggcgt gaacaacatc ctgctgcccc
361	agggcaggcc gccggtgttc cagcagcccg tcatctttct gggagcagac gtcactcacc
421	cccccgccgg ggatgggaag aagccctcca ttgccgccgt ggtgggcagc atggacgccc
481	accccaatcg ctactgcgcc accgtgcgcg tgcagcagca ccggcaggag atcatacaag
541	acctggccgc catggtccgc gagctcctca tccagttcta caagtccacg cgcttcaagc
601	ccacccgcat catcttctac cgcgacggtg tctctgaagg ccagttccag caggttctcc
661	accacgagtt gctggccatc cgtgaggcct gtatcaagct agaaaaagac taccagcccg
721	ggatcacctt catcgtggtg cagaagaggc accacacccg gctcttctgc actgacaaga
781	acgagcgggt tgggaaaagt ggaaacattc cagcaggcac gactgtggac acgaaaatca
841	cccaccccac cgagttcgac ttctacctgt gtagtcacgc tggcatccag gggacaagca
901	ggccttcgca ctatcacgtc ctctgggacg acaatcgttt ctcctctgat gagctgcaga
961	tcctaaccta ccagctgtgt cacacctacg tgcgctgcac acgctccgtg tccatcccag
1021	cgccagcata ctacgctcac ctggtggcct tccgggccag gtaccacctg gtggataagg
1081	aacatgacag tgctgaagga agccatacct ctgggcagag taacgggcga gaccaccaag
1141	cactggccaa ggcggtccag gttcaccaag acactctgcg caccatgtac tttgcttgcc
1201	caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
1261	ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
1321	tta

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