Construct: ORF ccsbBroadEn_11852

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009159.1_s300c1, BRDN0000386142
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AGO2 (27161)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 27161 AGO2 argonaute RISC catalytic co... XM_017013317.2 48.2% 48.2% 1_1215del
2 human 192669 AGO3 argonaute RISC catalytic co... XM_005270576.4 48.1% 58.4% (many diffs)
3 human 192669 AGO3 argonaute RISC catalytic co... XM_017000528.2 48.1% 58.4% (many diffs)
4 human 27161 AGO2 argonaute RISC catalytic co... XM_011516966.3 46.3% 46.3% 1_1308del
5 human 192669 AGO3 argonaute RISC catalytic co... NM_177422.3 44.9% 54.6% (many diffs)
6 human 192669 AGO3 argonaute RISC catalytic co... XM_011540882.3 44.9% 54.6% (many diffs)
7 human 192669 AGO3 argonaute RISC catalytic co... XM_017000524.2 44.9% 54.6% (many diffs)
8 human 192669 AGO3 argonaute RISC catalytic co... XM_017000525.2 44.9% 54.6% (many diffs)
9 human 192669 AGO3 argonaute RISC catalytic co... XM_017000526.2 44.9% 54.6% (many diffs)
10 human 192669 AGO3 argonaute RISC catalytic co... XM_017000527.2 44.9% 54.6% (many diffs)
11 human 27161 AGO2 argonaute RISC catalytic co... NM_012154.5 43.8% 43.8% 1_1446del
12 human 27161 AGO2 argonaute RISC catalytic co... XM_011516965.2 43.1% 43.1% 1_1488del
13 human 26523 AGO1 argonaute RISC component 1 XM_017001019.1 42.7% 47.8% (many diffs)
14 human 27161 AGO2 argonaute RISC catalytic co... XM_011516968.2 41% 41% 1_1623del
15 human 27161 AGO2 argonaute RISC catalytic co... NM_001164623.3 39.9% 39.9% 1_1446del;2168_2169ins102
16 human 26523 AGO1 argonaute RISC component 1 NM_001317123.1 38.5% 43.2% (many diffs)
17 human 26523 AGO1 argonaute RISC component 1 NM_012199.5 35.2% 39.4% (many diffs)
18 human 26523 AGO1 argonaute RISC component 1 XM_011541236.2 35.1% 39.3% (many diffs)
19 human 192669 AGO3 argonaute RISC catalytic co... XM_011540879.3 33.3% 40.4% (many diffs)
20 human 192669 AGO3 argonaute RISC catalytic co... NM_024852.4 32.7% 39.7% (many diffs)
21 human 192669 AGO3 argonaute RISC catalytic co... XM_005270575.4 32.7% 39.7% (many diffs)
22 human 192669 AGO3 argonaute RISC catalytic co... XM_017000523.2 32.7% 39.7% (many diffs)
23 mouse 236511 Ago1 argonaute RISC catalytic su... NM_001317174.1 43% 47.8% (many diffs)
24 mouse 236511 Ago1 argonaute RISC catalytic su... XM_011240521.1 42.9% 47.6% (many diffs)
25 mouse 239528 Ago2 argonaute RISC catalytic su... NM_153178.4 39.1% 43.8% (many diffs)
26 mouse 236511 Ago1 argonaute RISC catalytic su... NM_153403.3 35.5% 39.4% (many diffs)
27 mouse 236511 Ago1 argonaute RISC catalytic su... XM_011240520.2 35.3% 39.3% (many diffs)
28 mouse 236511 Ago1 argonaute RISC catalytic su... NM_001317173.1 34% 39.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1197
ORF length:
1131
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCC CATCCAGGGC CAGCCGTGCT TCTGCAAATA CGCGCAGGGG GCGGACAGCG
121TGGAGCCCAT GTTCCGGCAC CTGAAGAACA CGTATGCGGG CCTGCAGCTG GTGGTGGTCA
181TCCTGCCCGG CAAGACGCCC GTGTACGCCG AGGTCAAGCG CGTGGGAGAC ACGGTGCTGG
241GGATGGCCAC GCAGTGCGTG CAGATGAAGA ACGTGCAGAG GACCACGCCA CAGACCCTGT
301CCAACCTCTG CCTGAAGATC AACGTCAAGC TGGGAGGCGT GAACAACATC CTGCTGCCCC
361AGGGCAGGCC GCCGGTGTTC CAGCAGCCCG TCATCTTTCT GGGAGCAGAC GTCACTCACC
421CCCCCGCCGG GGATGGGAAG AAGCCCTCCA TTGCCGCCGT GGTGGGCAGC ATGGACGCCC
481ACCCCAATCG CTACTGCGCC ACCGTGCGCG TGCAGCAGCA CCGGCAGGAG ATCATACAAG
541ACCTGGCCGC CATGGTCCGC GAGCTCCTCA TCCAGTTCTA CAAGTCCACG CGCTTCAAGC
601CCACCCGCAT CATCTTCTAC CGCGACGGTG TCTCTGAAGG CCAGTTCCAG CAGGTTCTCC
661ACCACGAGTT GCTGGCCATC CGTGAGGCCT GTATCAAGCT AGAAAAAGAC TACCAGCCCG
721GGATCACCTT CATCGTGGTG CAGAAGAGGC ACCACACCCG GCTCTTCTGC ACTGACAAGA
781ACGAGCGGGT TGGGAAAAGT GGAAACATTC CAGCAGGCAC GACTGTGGAC ACGAAAATCA
841CCCACCCCAC CGAGTTCGAC TTCTACCTGT GTAGTCACGC TGGCATCCAG GGGACAAGCA
901GGCCTTCGCA CTATCACGTC CTCTGGGACG ACAATCGTTT CTCCTCTGAT GAGCTGCAGA
961TCCTAACCTA CCAGCTGTGT CACACCTACG TGCGCTGCAC ACGCTCCGTG TCCATCCCAG
1021CGCCAGCATA CTACGCTCAC CTGGTGGCCT TCCGGGCCAG GTACCACCTG GTGGATAAGG
1081AACATGACAG TGCTGAAGGA AGCCATACCT CTGGGCAGAG TAACGGGCGA GACCACCAAG
1141CACTGGCCAA GGCGGTCCAG GTTCACCAAG ACACTCTGCG CACCATGTAC TTTGCTTGCC
1201CAACTTTCTT GTACAAAGTt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
1261aac

Download FASTA (ORF) (Full)