Transcript: Human NM_001318028.2

Homo sapiens protein phosphatase 4 regulatory subunit 2 (PPP4R2), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
PPP4R2 (151987)
Length:
725
CDS:
228..437

Additional Resources:

NCBI RefSeq record:
NM_001318028.2
NBCI Gene record:
PPP4R2 (151987)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001318028.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 543 3UTR 100% 4.950 2.475 Y n/a
2 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 486 3UTR 100% 5.625 2.813 Y KLHL30 n/a
3 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 486 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001318028.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13282 pDONR223 100% 15.6% 12.6% None (many diffs) n/a
2 ccsbBroad304_13282 pLX_304 0% 15.6% 12.6% V5 (many diffs) n/a
3 TRCN0000477746 TAAGAATTATATATTCTGTGGACA pLX_317 37.9% 15.6% 12.6% V5 (many diffs) n/a
4 ccsbBroadEn_05060 pDONR223 100% 14.2% 11.5% None (many diffs) n/a
5 ccsbBroad304_05060 pLX_304 0% 14.2% 11.5% V5 (many diffs) n/a
6 TRCN0000477613 GTGGCCATGGTCTTCGTCTAACTT pLX_317 35% 14.2% 11.5% V5 (many diffs) n/a
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