Transcript: Human NM_001318897.2

Homo sapiens four and a half LIM domains 2 (FHL2), transcript variant 9, mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
FHL2 (2274)
Length:
4375
CDS:
307..804

Additional Resources:

NCBI RefSeq record:
NM_001318897.2
NBCI Gene record:
FHL2 (2274)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001318897.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000005773 CGACTGCTTTAACTGTAAGAA pLKO.1 702 CDS 100% 5.625 7.875 N FHL2 n/a
2 TRCN0000273528 CGACTGCTTTAACTGTAAGAA pLKO_005 702 CDS 100% 5.625 7.875 N FHL2 n/a
3 TRCN0000005772 CCCTGCTATGAGAAACAACAT pLKO.1 418 CDS 100% 4.950 6.930 N FHL2 n/a
4 TRCN0000285011 CCCAAAGACAATCAGAATTTC pLKO_005 391 CDS 100% 13.200 9.240 N FHL2 n/a
5 TRCN0000285010 CCAATTGGAACCAAGAGTTTC pLKO_005 367 CDS 100% 10.800 7.560 N FHL2 n/a
6 TRCN0000005774 CGAATCTCTCTTTGGCAAGAA pLKO.1 172 5UTR 100% 4.950 3.465 N FHL2 n/a
7 TRCN0000273585 CGAATCTCTCTTTGGCAAGAA pLKO_005 172 5UTR 100% 4.950 3.465 N FHL2 n/a
8 TRCN0000005771 GAGACTTTCTTCTAGTGCTTT pLKO.1 906 3UTR 100% 4.950 3.465 N FHL2 n/a
9 TRCN0000273529 GAGACTTTCTTCTAGTGCTTT pLKO_005 906 3UTR 100% 4.950 3.465 N FHL2 n/a
10 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 1451 3UTR 100% 4.950 2.475 Y ERAP2 n/a
11 TRCN0000155229 GATCAAGACCATCCTGGCTAA pLKO.1 1506 3UTR 100% 4.050 2.025 Y INTS7 n/a
12 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 1452 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001318897.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00568 pDONR223 100% 59.1% 59.1% None 0_1ins342 n/a
2 ccsbBroad304_00568 pLX_304 0% 59.1% 59.1% V5 0_1ins342 n/a
3 TRCN0000466342 ATGTCCCGGCCCCCCTTAATCACA pLX_317 45.7% 59.1% 59.1% V5 0_1ins342 n/a
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