Transcript: Human NM_001321985.2

Homo sapiens ectonucleoside triphosphate diphosphohydrolase 5 (inactive) (ENTPD5), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
ENTPD5 (957)
Length:
5915
CDS:
394..1680

Additional Resources:

NCBI RefSeq record:
NM_001321985.2
NBCI Gene record:
ENTPD5 (957)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321985.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000229314 GATCCGACGAAGGCATATTAG pLKO_005 899 CDS 100% 13.200 18.480 N ENTPD5 n/a
2 TRCN0000229313 AGCACCTTGTATGGAATTATG pLKO_005 529 CDS 100% 13.200 9.240 N ENTPD5 n/a
3 TRCN0000218711 AGCCTAGAGATTTAGGTTTAA pLKO_005 1780 3UTR 100% 13.200 9.240 N ENTPD5 n/a
4 TRCN0000229315 TCACTTCCTTTGAGATGTTTA pLKO_005 1067 CDS 100% 13.200 9.240 N ENTPD5 n/a
5 TRCN0000080774 GCTCACAAAGAAAGTGAACAA pLKO.1 1593 CDS 100% 4.950 3.465 N Entpd5 n/a
6 TRCN0000327094 GCTCACAAAGAAAGTGAACAA pLKO_005 1593 CDS 100% 4.950 3.465 N Entpd5 n/a
7 TRCN0000050479 GCAGACTTGGTTTGAGGGTAT pLKO.1 471 CDS 100% 4.050 2.835 N ENTPD5 n/a
8 TRCN0000050478 GCCCTGTTAAAGGATGGCTTT pLKO.1 1546 CDS 100% 4.050 2.835 N ENTPD5 n/a
9 TRCN0000050482 GAAGGCATATTAGCTTGGGTT pLKO.1 907 CDS 100% 2.640 1.848 N ENTPD5 n/a
10 TRCN0000050481 GCCAGCACCTTGTATGGAATT pLKO.1 526 CDS 100% 0.000 0.000 N ENTPD5 n/a
11 TRCN0000229316 ATGCTTTCTCTTACTATTATG pLKO_005 1373 CDS 100% 13.200 7.920 N ENTPD5 n/a
12 TRCN0000050480 GCTCTATACACATAGTTACTT pLKO.1 1101 CDS 100% 5.625 3.938 N ENTPD5 n/a
13 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3119 3UTR 100% 5.625 2.813 Y KLHL30 n/a
14 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3119 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321985.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15379 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_15379 pLX_304 0% 100% 100% V5 n/a
3 ccsbBroadEn_13825 pDONR223 100% 94.2% 93.2% None (many diffs) n/a
4 ccsbBroad304_13825 pLX_304 0% 94.2% 93.2% V5 (many diffs) n/a
5 TRCN0000472576 CTGATAGGACTATTAAGCTCCGCA pLX_317 31.2% 94.2% 93.2% V5 (many diffs) n/a
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