Transcript: Mouse NM_001331140.1

Mus musculus transcription factor 7 like 2, T cell specific, HMG box (Tcf7l2), transcript variant 15, mRNA.

Source:
NCBI, updated 2017-06-11
Taxon:
Mus musculus (mouse)
Gene:
Tcf7l2 (21416)
Length:
4115
CDS:
533..2317

Additional Resources:

NCBI RefSeq record:
NM_001331140.1
NBCI Gene record:
Tcf7l2 (21416)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001331140.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000374009 TGGCCGAATGCACATTGAAAG pLKO_005 1557 CDS 100% 10.800 15.120 N Tcf7l2 n/a
2 TRCN0000012179 GCTCCGAAAGTTTCCGAGATA pLKO.1 744 CDS 100% 4.950 3.960 N Tcf7l2 n/a
3 TRCN0000421340 CAAGATGGAGGGCTCTTTAAG pLKO_005 800 CDS 100% 13.200 9.240 N Tcf7l2 n/a
4 TRCN0000321291 CCAGATATCTCTCCATATTAC pLKO_005 1172 CDS 100% 13.200 9.240 N Tcf7l2 n/a
5 TRCN0000012178 CCTTAGCGTAAGCATCTTATA pLKO.1 3100 3UTR 100% 13.200 9.240 N Tcf7l2 n/a
6 TRCN0000378996 CGCCGTGGCTACATGAGTTAA pLKO_005 2429 3UTR 100% 13.200 9.240 N Tcf7l2 n/a
7 TRCN0000321293 TATGGAGTTCATTGGTCAATA pLKO_005 2454 3UTR 100% 13.200 9.240 N Tcf7l2 n/a
8 TRCN0000416397 ACGAGCTGATCTCCTTCAAAG pLKO_005 579 CDS 100% 10.800 7.560 N Tcf7l2 n/a
9 TRCN0000321292 GGTCTGCACGGGATAACTATG pLKO_005 1701 CDS 100% 10.800 7.560 N Tcf7l2 n/a
10 TRCN0000012180 GCTGACAGTCAACGCATCTAT pLKO.1 1300 CDS 100% 5.625 3.938 N Tcf7l2 n/a
11 TRCN0000321290 GCTGACAGTCAACGCATCTAT pLKO_005 1300 CDS 100% 5.625 3.938 N Tcf7l2 n/a
12 TRCN0000012182 CCCTCCAGATATCTCTCCATA pLKO.1 1168 CDS 100% 4.950 3.465 N Tcf7l2 n/a
13 TRCN0000012181 GTTTCCTAAATCCTTGCCTTT pLKO.1 1782 CDS 100% 4.050 2.835 N Tcf7l2 n/a
14 TRCN0000282444 CTCCGCACCCTCCAGATATAT pLKO_005 1161 CDS 100% 15.000 10.500 N TCF7L2 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001331140.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01651 pDONR223 100% 70.4% 70.7% None (many diffs) n/a
2 ccsbBroad304_01651 pLX_304 49.6% 70.4% 70.7% V5 (many diffs) n/a
3 TRCN0000480255 GCCTCGCGGCGTGACGATTTCTTC pLX_317 23% 70.4% 70.7% V5 (many diffs) n/a
Download CSV