Transcript: Human NM_001348365.1

Homo sapiens HECT domain E3 ubiquitin protein ligase 2 (HECTD2), transcript variant 5, mRNA.

Source:
NCBI, updated 2018-07-01
Taxon:
Homo sapiens (human)
Gene:
HECTD2 (143279)
Length:
4842
CDS:
371..2380

Additional Resources:

NCBI RefSeq record:
NM_001348365.1
NBCI Gene record:
HECTD2 (143279)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001348365.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000412949 GTCAGTACCCATTCGTTATTT pLKO_005 1155 CDS 100% 15.000 21.000 N Hectd2 n/a
2 TRCN0000432306 AGTTAAGTCATCAGGAGATTG pLKO_005 472 CDS 100% 10.800 15.120 N HECTD2 n/a
3 TRCN0000417675 TTATCTAACAACGTTTGATTC pLKO_005 511 CDS 100% 10.800 8.640 N HECTD2 n/a
4 TRCN0000419398 ACCAATTTGCCTTGATGTTAG pLKO_005 331 5UTR 100% 10.800 7.560 N HECTD2 n/a
5 TRCN0000429438 CAGTCCTTCCAGAACCTATTC pLKO_005 402 CDS 100% 10.800 7.560 N HECTD2 n/a
6 TRCN0000430786 CCATTGAAGACTCTGGGATTA pLKO_005 585 CDS 100% 10.800 7.560 N HECTD2 n/a
7 TRCN0000420007 GATGCATCATCATCCGAAATG pLKO_005 374 CDS 100% 10.800 7.560 N HECTD2 n/a
8 TRCN0000007762 GCCTCATTTAACACCATTGAA pLKO.1 572 CDS 100% 5.625 3.938 N HECTD2 n/a
9 TRCN0000007759 GCTGTGTATGATACCTTACTT pLKO.1 623 CDS 100% 5.625 3.938 N HECTD2 n/a
10 TRCN0000007760 CCCAGAATTAAATGCTGCATT pLKO.1 535 CDS 100% 4.950 3.465 N HECTD2 n/a
11 TRCN0000007761 CGAAGAAAGAAGCTGCTGAAA pLKO.1 252 5UTR 100% 4.950 3.465 N HECTD2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001348365.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000491311 AGACCCACCAAAACAATGAAACTA pLX_317 9.4% 86.2% 86.2% V5 (not translated due to prior stop codon) 0_1ins321 n/a
2 TRCN0000488652 GGGGAAACATCGGCAACATGCCAC pLX_317 14.4% 86.1% 86.1% V5 0_1ins321;2007_2008insG n/a
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