Transcript: Human NM_001348802.2

Homo sapiens zinc finger and BTB domain containing 20 (ZBTB20), transcript variant 11, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
ZBTB20 (26137)
Length:
27232
CDS:
727..2733

Additional Resources:

NCBI RefSeq record:
NM_001348802.2
NBCI Gene record:
ZBTB20 (26137)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001348802.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000222180 CGCAGACAAACCAGCTAGAAA pLKO.1 1769 CDS 100% 5.625 7.875 N ZBTB20 n/a
2 TRCN0000086330 CGGGTCATCTGATTGTGACAT pLKO.1 693 5UTR 100% 4.950 6.930 N Zbtb20 n/a
3 TRCN0000312851 AGCTATGGCACTAGAATTTAA pLKO_005 2798 3UTR 100% 15.000 10.500 N Zbtb20 n/a
4 TRCN0000086328 GCCTGCTGGTACATTACATTT pLKO.1 2899 3UTR 100% 13.200 9.240 N Zbtb20 n/a
5 TRCN0000222178 CCCAGCAAAGTTTGACCAAAT pLKO.1 2667 CDS 100% 10.800 7.560 N ZBTB20 n/a
6 TRCN0000222179 CCAGTGTAGTATCTGCAACAA pLKO.1 2409 CDS 100% 4.950 3.465 N ZBTB20 n/a
7 TRCN0000086329 GCCCAGCAAAGTTTGACCAAA pLKO.1 2666 CDS 100% 4.950 3.465 N Zbtb20 n/a
8 TRCN0000222182 CACTGTTATCACTGTCAGCAA pLKO.1 1836 CDS 100% 2.640 1.848 N ZBTB20 n/a
9 TRCN0000222181 CGCTCCTTCTCCTTAAAGGAT pLKO.1 2344 CDS 100% 0.300 0.210 N ZBTB20 n/a
10 TRCN0000166364 CACACACACACACACACACAA pLKO.1 10601 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001348802.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07993 pDONR223 100% 99.9% 99.8% None 1409C>T n/a
2 ccsbBroad304_07993 pLX_304 0% 99.9% 99.8% V5 1409C>T n/a
3 TRCN0000476850 ACTACCACCCCAGAAATTGACATC pLX_317 19% 99.9% 99.8% V5 1409C>T n/a
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