Transcript: Human NM_001352752.2

Homo sapiens protein tyrosine kinase 2 (PTK2), transcript variant 63, mRNA.

Source:
NCBI, updated 2019-08-13
Taxon:
Homo sapiens (human)
Gene:
PTK2 (5747)
Length:
2885
CDS:
294..1319

Additional Resources:

NCBI RefSeq record:
NM_001352752.2
NBCI Gene record:
PTK2 (5747)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001352752.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000001620 CCGGTCGAATGATAAGGTGTA pLKO.1 914 CDS 100% 4.050 5.670 N PTK2 n/a
2 TRCN0000194944 CACACTTTGATTTGGGTTCAT pLKO.1 1481 3UTR 100% 4.950 3.960 N PTK2 n/a
3 TRCN0000121321 CTTGGCCCTGAGGACATTATT pLKO.1 1034 CDS 100% 15.000 10.500 N PTK2 n/a
4 TRCN0000195014 CAATATGCTAATCCCACTTTA pLKO.1 1816 3UTR 100% 13.200 9.240 N PTK2 n/a
5 TRCN0000121127 CGTGTGGATATGTGAAGCATT pLKO.1 1922 3UTR 100% 4.950 3.465 N PTK2 n/a
6 TRCN0000195611 CGATGTCATTGACCAAGCAAG pLKO.1 1265 CDS 100% 4.050 2.835 N PTK2 n/a
7 TRCN0000001621 CGACAGCAACAGGAAATGGAA pLKO.1 663 CDS 100% 3.000 2.100 N PTK2 n/a
8 TRCN0000121317 GCAATGTTATTTCTCTTGGAA pLKO.1 2018 3UTR 100% 3.000 2.100 N PTK2 n/a
9 TRCN0000001617 GAGAGCATGAAGCAAAGAATT pLKO.1 1704 3UTR 100% 0.000 0.000 N PTK2 n/a
10 TRCN0000121207 CCCAGGAGAGAATGAAGCAAA pLKO.1 2199 3UTR 100% 4.950 2.970 N PTK2 n/a
11 TRCN0000344602 CCCAGGAGAGAATGAAGCAAA pLKO_005 2199 3UTR 100% 4.950 2.970 N PTK2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001352752.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13934 pDONR223 100% 50% 49.7% None (many diffs) n/a
2 ccsbBroad304_13934 pLX_304 0% 50% 49.7% V5 (many diffs) n/a
3 TRCN0000465393 CCGAGCTAACCTATAGCACCACCA pLX_317 11.8% 50% 49.7% V5 (many diffs) n/a
4 ccsbBroadEn_14818 pDONR223 0% 32.4% 32.4% None 0_1ins2070;428_429ins63 n/a
5 ccsbBroad304_14818 pLX_304 0% 32.4% 32.4% V5 0_1ins2070;428_429ins63 n/a
6 TRCN0000471115 ACCCGTCAGACGGGGAGAATATCA pLX_317 13% 32.4% 32.4% V5 0_1ins2070;428_429ins63 n/a
7 TRCN0000489116 AGGTTGAACTATACACTATACCTG pLX_317 12.9% 32.4% 32.4% V5 (not translated due to prior stop codon) 0_1ins2070;428_429ins63 n/a
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