Construct: ORF TRCN0000489116

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020624.1_s317c1
DNA Barcode:
AGGTTGAACTATACACTATACCTG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
PTK2 (5747)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489116
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5747 PTK2 protein tyrosine kinase 2 NM_001352694.2 100% 100%
2 human 5747 PTK2 protein tyrosine kinase 2 NM_153831.4 100% 100%
3 human 5747 PTK2 protein tyrosine kinase 2 NM_001352718.2 99.7% 99.7% 2709_2717delGCCATGGAG
4 human 5747 PTK2 protein tyrosine kinase 2 NM_001352719.2 99.7% 99.7% 2709_2717delGCCATGGAG
5 human 5747 PTK2 protein tyrosine kinase 2 NM_001352720.2 99.7% 99.7% 2709_2717delGCCATGGAG
6 human 5747 PTK2 protein tyrosine kinase 2 NM_001352717.2 99.4% 99.4% 1177_1194del
7 human 5747 PTK2 protein tyrosine kinase 2 XM_017013669.2 99.3% 99.2% 1235_1255del
8 human 5747 PTK2 protein tyrosine kinase 2 NM_001352715.2 99.1% 99.1% 1177_1194del;2727_2735delGCCATGGAG
9 human 5747 PTK2 protein tyrosine kinase 2 XM_017013664.2 99.1% 99.1% 1177_1194del;2727_2735delGCCATGGAG
10 human 5747 PTK2 protein tyrosine kinase 2 NM_001352713.2 99% 98.9% 1235_1255del;2730_2738delGCCATGGAG
11 human 5747 PTK2 protein tyrosine kinase 2 NM_001352714.2 99% 98.9% 1235_1255del;2730_2738delGCCATGGAG
12 human 5747 PTK2 protein tyrosine kinase 2 NM_001199649.2 98.7% 98.6% 2603_2632del;2739_2747delGCCATGGAG
13 human 5747 PTK2 protein tyrosine kinase 2 NM_001352706.2 98.5% 98.4% 1177_1194del;1253_1273del;2748_2756delGCCATGGAG
14 human 5747 PTK2 protein tyrosine kinase 2 NM_001352707.2 98.5% 98.4% 1177_1194del;1253_1273del;2748_2756delGCCATGGAG
15 human 5747 PTK2 protein tyrosine kinase 2 NM_001352708.2 98.5% 98.4% 1177_1194del;1253_1273del;2748_2756delGCCATGGAG
16 human 5747 PTK2 protein tyrosine kinase 2 NM_001352709.2 98.5% 98.4% 1177_1194del;1253_1273del;2748_2756delGCCATGGAG
17 human 5747 PTK2 protein tyrosine kinase 2 NM_001352710.2 98.5% 98.4% 1177_1194del;1253_1273del;2748_2756delGCCATGGAG
18 human 5747 PTK2 protein tyrosine kinase 2 NM_001352711.2 98.4% 98.3% (many diffs)
19 human 5747 PTK2 protein tyrosine kinase 2 NM_005607.5 97.9% 97.9% 1_66del
20 human 5747 PTK2 protein tyrosine kinase 2 NM_001352725.2 97.7% 97.7% 2498_2499ins63;2646_2654delGCCATGGAG
21 human 5747 PTK2 protein tyrosine kinase 2 NM_001352705.2 97.3% 97.2% (many diffs)
22 human 5747 PTK2 protein tyrosine kinase 2 NM_001352704.2 96.6% 96.6% 1177_1278del;2811_2819delGCCATGGAG
23 human 5747 PTK2 protein tyrosine kinase 2 NM_001352728.2 96.5% 96.5% 867_868ins108
24 human 5747 PTK2 protein tyrosine kinase 2 NM_001352721.2 96.5% 96.4% (many diffs)
25 human 5747 PTK2 protein tyrosine kinase 2 NM_001352722.2 96.5% 96.4% (many diffs)
26 human 5747 PTK2 protein tyrosine kinase 2 NM_001352723.2 96.5% 96.4% (many diffs)
27 human 5747 PTK2 protein tyrosine kinase 2 NM_001352712.2 96.2% 96.1% 1177_1278del;1337_1357del
28 human 5747 PTK2 protein tyrosine kinase 2 NM_001352699.2 95.9% 95.8% 1177_1278del;1337_1357del;2832_2840delGCCATGGAG
29 human 5747 PTK2 protein tyrosine kinase 2 NM_001352700.2 95.9% 95.8% 1177_1278del;1337_1357del;2832_2840delGCCATGGAG
30 human 5747 PTK2 protein tyrosine kinase 2 NM_001352701.2 95.9% 95.8% 1177_1278del;1337_1357del;2832_2840delGCCATGGAG
31 human 5747 PTK2 protein tyrosine kinase 2 NM_001352702.2 95.9% 95.8% 1177_1278del;1337_1357del;2832_2840delGCCATGGAG
32 human 5747 PTK2 protein tyrosine kinase 2 NM_001352703.2 95.9% 95.8% 1177_1278del;1337_1357del;2832_2840delGCCATGGAG
33 human 5747 PTK2 protein tyrosine kinase 2 NM_001352724.2 95.9% 95.8% (many diffs)
34 human 5747 PTK2 protein tyrosine kinase 2 XM_017013657.2 95.7% 95.7% 1_132del;2841_2849delGCCATGGAG
35 human 5747 PTK2 protein tyrosine kinase 2 XM_024447205.1 95.3% 95.3% 1_153del
36 human 5747 PTK2 protein tyrosine kinase 2 XM_017013656.2 95.3% 95.2% 1_132del;1367_1387del
37 human 5747 PTK2 protein tyrosine kinase 2 NM_001352726.2 95.1% 95% (many diffs)
38 human 5747 PTK2 protein tyrosine kinase 2 XM_024447211.1 95.1% 95% (many diffs)
39 human 5747 PTK2 protein tyrosine kinase 2 XM_024447204.1 95.1% 95.1% 1_153del;2862_2870delGCCATGGAG
40 human 5747 PTK2 protein tyrosine kinase 2 XM_017013654.2 95.1% 95% 1_132del;1367_1387del;2862_2870delGCCATGGAG
41 human 5747 PTK2 protein tyrosine kinase 2 NM_001352695.2 94.9% 94.9% 1177_1278del;2600_2601ins63
42 human 5747 PTK2 protein tyrosine kinase 2 XM_024447203.1 94.8% 94.8% 1_153del;1330_1347del
43 human 5747 PTK2 protein tyrosine kinase 2 NM_001352698.2 94.8% 94.7% (many diffs)
44 human 5747 PTK2 protein tyrosine kinase 2 XM_024447202.1 94.7% 94.6% 1_153del;1388_1408del
45 human 5747 PTK2 protein tyrosine kinase 2 XM_024447201.1 94.6% 94.6% 1_153del;1330_1347del;2880_2888delGCCATGGAG
46 human 5747 PTK2 protein tyrosine kinase 2 XM_017013653.2 94.6% 94.5% (many diffs)
47 human 5747 PTK2 protein tyrosine kinase 2 XM_024447200.1 94.5% 94.4% 1_153del;1388_1408del;2883_2891delGCCATGGAG
48 human 5747 PTK2 protein tyrosine kinase 2 XM_024447199.1 94% 93.9% (many diffs)
49 human 5747 PTK2 protein tyrosine kinase 2 NM_001352727.2 94% 93.9% (many diffs)
50 human 5747 PTK2 protein tyrosine kinase 2 XM_024447207.1 93.2% 93.2% 1_153del;2651_2652ins63;2799_2807delGCCATGGAG
51 human 5747 PTK2 protein tyrosine kinase 2 NM_001352729.2 93.1% 93% (many diffs)
52 human 5747 PTK2 protein tyrosine kinase 2 XM_017013666.1 92.7% 92.7% 1_132del;999_1000ins108
53 human 5747 PTK2 protein tyrosine kinase 2 NM_001352716.2 92.7% 92.6% (many diffs)
54 human 5747 PTK2 protein tyrosine kinase 2 XM_024447206.1 92.1% 92% (many diffs)
55 human 5747 PTK2 protein tyrosine kinase 2 XM_024447209.1 91.2% 91.1% (many diffs)
56 human 5747 PTK2 protein tyrosine kinase 2 XM_024447208.1 90.7% 90.7% (many diffs)
57 human 5747 PTK2 protein tyrosine kinase 2 NM_001352697.2 90.6% 90.5% (many diffs)
58 human 5747 PTK2 protein tyrosine kinase 2 NM_001352736.2 89.6% 89.6% 0_1ins303;874_891del;2424_2432delGCCATGGAG
59 human 5747 PTK2 protein tyrosine kinase 2 NM_001352741.2 89% 88.4% 0_1ins172;23_24ins167;2370_2378delGCCATGGAG
60 human 5747 PTK2 protein tyrosine kinase 2 XM_024447212.1 89% 88.4% 0_1ins172;23_24ins167;2370_2378delGCCATGGAG
61 human 5747 PTK2 protein tyrosine kinase 2 XM_024447210.1 88.9% 88.8% (many diffs)
62 human 5747 PTK2 protein tyrosine kinase 2 NM_001352740.2 88.5% 87.9% (many diffs)
63 human 5747 PTK2 protein tyrosine kinase 2 NM_001352739.2 88.4% 87.7% (many diffs)
64 human 5747 PTK2 protein tyrosine kinase 2 XM_017013684.2 88.4% 87.7% (many diffs)
65 human 5747 PTK2 protein tyrosine kinase 2 NM_001352738.2 87.9% 87.2% (many diffs)
66 human 5747 PTK2 protein tyrosine kinase 2 NM_001316342.2 87.9% 87.2% (many diffs)
67 human 5747 PTK2 protein tyrosine kinase 2 NM_001352696.2 87.9% 87.2% (many diffs)
68 human 5747 PTK2 protein tyrosine kinase 2 NM_001352737.2 86.4% 85.9% 0_1ins172;23_24ins167;838_939del
69 human 5747 PTK2 protein tyrosine kinase 2 NM_001352733.2 86.2% 85.6% (many diffs)
70 human 5747 PTK2 protein tyrosine kinase 2 NM_001352742.2 85.9% 85.2% (many diffs)
71 human 5747 PTK2 protein tyrosine kinase 2 NM_001352743.2 85.9% 85.2% (many diffs)
72 human 5747 PTK2 protein tyrosine kinase 2 NM_001352732.2 85.9% 85.2% (many diffs)
73 human 5747 PTK2 protein tyrosine kinase 2 NM_001352731.2 85.7% 85% (many diffs)
74 human 5747 PTK2 protein tyrosine kinase 2 NM_001352730.2 85.6% 85% (many diffs)
75 human 5747 PTK2 protein tyrosine kinase 2 NM_001352745.2 85% 84.3% (many diffs)
76 human 5747 PTK2 protein tyrosine kinase 2 NM_001352744.2 84.5% 83.8% (many diffs)
77 human 5747 PTK2 protein tyrosine kinase 2 XM_017013688.2 84.5% 83.8% (many diffs)
78 human 5747 PTK2 protein tyrosine kinase 2 NM_001352734.2 83.7% 83.1% (many diffs)
79 human 5747 PTK2 protein tyrosine kinase 2 NM_001352735.2 83.7% 83.1% (many diffs)
80 human 5747 PTK2 protein tyrosine kinase 2 NM_001352746.2 82.5% 81.9% (many diffs)
81 human 5747 PTK2 protein tyrosine kinase 2 NR_148036.2 59.2% (many diffs)
82 human 5747 PTK2 protein tyrosine kinase 2 NR_148037.2 58.9% (many diffs)
83 human 5747 PTK2 protein tyrosine kinase 2 NR_148038.2 56.9% (many diffs)
84 human 5747 PTK2 protein tyrosine kinase 2 NR_148039.2 54.8% (many diffs)
85 human 5747 PTK2 protein tyrosine kinase 2 NM_001352747.2 54.7% 54.7% 0_1ins1422;1287_1295delGCCATGGAG
86 human 5747 PTK2 protein tyrosine kinase 2 NM_001352748.2 45.6% 44.3% (many diffs)
87 human 5747 PTK2 protein tyrosine kinase 2 NM_001352750.2 34.4% 34.4% 0_1ins2070
88 human 5747 PTK2 protein tyrosine kinase 2 NM_001352749.2 34.3% 34.3% 0_1ins2070;639_647delGCCATGGAG
89 human 5747 PTK2 protein tyrosine kinase 2 NM_001352752.2 32.4% 32.4% 0_1ins2070;428_429ins63
90 human 5747 PTK2 protein tyrosine kinase 2 NM_001352751.2 32.3% 32.3% 0_1ins2070;428_429ins63;576_584delGCCATGGAG
91 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 NM_007982.2 90.7% 97.3% (many diffs)
92 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520433.2 90.5% 97% (many diffs)
93 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_017316429.1 89.4% 95.7% (many diffs)
94 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520432.2 87.1% 93.4% (many diffs)
95 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520424.2 87.1% 93.3% (many diffs)
96 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520425.2 87.1% 93.3% (many diffs)
97 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520426.2 87.1% 93.3% (many diffs)
98 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520427.3 87.1% 93.3% (many diffs)
99 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520428.2 87.1% 93.3% (many diffs)
100 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520429.2 87.1% 93.3% (many diffs)
101 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520430.2 87.1% 93.3% (many diffs)
102 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520431.2 87.1% 93.3% (many diffs)
103 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520435.3 87.1% 93.3% (many diffs)
104 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520422.1 86.9% 93.1% (many diffs)
105 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520423.1 86.9% 93.1% (many diffs)
106 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_017316428.1 86.5% 92.7% (many diffs)
107 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520421.1 86.3% 92.4% (many diffs)
108 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520420.1 85.8% 91.9% (many diffs)
109 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520418.1 84.2% 90.3% (many diffs)
110 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_011245452.2 83.9% 89.9% (many diffs)
111 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520415.2 83.7% 89.7% (many diffs)
112 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520416.2 83.7% 89.7% (many diffs)
113 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_011245453.1 82.1% 87.9% (many diffs)
114 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 NM_001130409.1 79.2% 82.7% (many diffs)
115 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520434.2 78.3% 84.3% (many diffs)
116 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XR_383981.2 50.4% (many diffs)
117 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XR_001781493.1 32.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3228
ORF length:
3156
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catggcagct gcttaccttg accccaactt gaatcacaca ccaaattcga
121gtactaagac tcacctgggt actggtatgg aacgttctcc tggtgcaatg gagcgagtat
181taaaggtctt tcattatttt gaaagcaata gtgagccaac cacctgggcc agtattatca
241ggcatggaga tgctactgat gtcaggggca tcattcagaa gatagtggac agtcacaaag
301taaagcatgt ggcctgctat ggattccgcc tcagtcacct gcggtcagag gaggttcact
361ggcttcacgt ggatatgggc gtctccagtg tgagggagaa gtatgagctt gctcacccac
421cagaggagtg gaaatatgaa ttgagaattc gttatttgcc aaaaggattt ctaaaccagt
481ttactgaaga taagccaact ttgaatttct tctatcaaca ggtgaagagc gattatatgt
541tagagatagc tgatcaagtg gaccaggaaa ttgctttgaa gttgggttgt ctagaaatac
601ggcgatcata ctgggagatg cggggcaatg cactagaaaa gaagtctaac tatgaagtat
661tagaaaaaga tgttggttta aagcgatttt ttcctaagag tttactggat tctgtcaagg
721ccaaaacact aagaaaactg atccaacaaa catttagaca atttgccaac cttaatagag
781aagaaagtat tctgaaattc tttgagatcc tgtctccagt ctacagattt gataaggaat
841gcttcaagtg tgctcttggt tcaagctgga ttatttcagt ggaactggca atcggcccag
901aagaaggaat cagttaccta acggacaagg gctgcaatcc cacacatctt gctgacttca
961ctcaagtgca aaccattcag tattcaaaca gtgaagacaa ggacagaaaa ggaatgctac
1021aactaaaaat agcaggtgca cccgagcctc tgacagtgac ggcaccatcc ctaaccattg
1081cggagaatat ggctgaccta atagatgggt actgccggct ggtgaatgga acctcgcagt
1141catttatcat cagacctcag aaagaaggtg aacgggcttt gccatcaata ccaaagttgg
1201ccaacagcga aaagcaaggc atgcggacac acgccgtctc tgtgtcagaa acagatgatt
1261atgctgagat tatagatgaa gaagatactt acaccatgcc ctcaaccagg gattatgaga
1321ttcaaagaga aagaatagaa cttggacgat gtattggaga aggccaattt ggagatgtac
1381atcaaggcat ttatatgagt ccagagaatc cagctttggc ggttgcaatt aaaacatgta
1441aaaactgtac ttcggacagc gtgagagaga aatttcttca agaagcctta acaatgcgtc
1501agtttgacca tcctcatatt gtgaagctga ttggagtcat cacagagaat cctgtctgga
1561taatcatgga gctgtgcaca cttggagagc tgaggtcatt tttgcaagta aggaaataca
1621gtttggatct agcatctttg atcctgtatg cctatcagct tagtacagct cttgcatatc
1681tagagagcaa aagatttgta cacagggaca ttgctgctcg gaatgttctg gtgtcctcaa
1741atgattgtgt aaaattagga gactttggat tatcccgata tatggaagat agtacttact
1801acaaagcttc caaaggaaaa ttgcctatta aatggatggc tccagagtca atcaattttc
1861gacgttttac ctcagctagt gacgtatgga tgtttggtgt gtgtatgtgg gagatactga
1921tgcatggtgt gaagcctttt caaggagtga agaacaatga tgtaatcggt cgaattgaaa
1981atggggaaag attaccaatg cctccaaatt gtcctcctac cctctacagc cttatgacga
2041aatgctgggc ctatgacccc agcaggcggc ccaggtttac tgaacttaaa gctcagctca
2101gcacaatcct ggaggaagag aaggctcagc aagaagagcg catgaggatg gagtccagaa
2161gacaggccac agtgtcctgg gactccggag ggtctgatga agcaccgccc aagcccagca
2221gaccgggtta tcccagtccg aggtccagcg aaggatttta tcccagccca cagcacatgg
2281tacaaaccaa tcattaccag gtttctggct accctggttc acatggaatc acagccatgg
2341ctggcagcat ctatccaggt caggcatctc ttttggacca aacagattca tggaatcata
2401gacctcagga gatagcaatg tggcagccca atgtggagga ctctacagta ttggacctgc
2461gagggattgg gcaagtgttg ccaacccatc tgatggaaga gcgtctaatc cgacagcaac
2521aggaaatgga agaagatcag cgctggctgg aaaaagagga aagatttctg aaacctgatg
2581tgagactctc tcgaggcagt attgacaggg aggatggaag tcttcagggt ccgattggaa
2641accaacatat atatcagcct gtgggtaaac cagatcctgc agctccacca aagaaaccgc
2701ctcgccctgg agctcccggt catctgggaa gccttgccag cctcagcagc cctgctgaca
2761gctacaacga gggtgtcaag cttcagcccc aggaaatcag cccccctcct actgccaacc
2821tggaccgGTC GAATGATAAG GTGTACGAGA ATGTGACGGG CCTGGTGAAA GCTGTCATCG
2881AGATGTCCAG TAAAATCCAG CCAGCCCCAC CAGAGGAGTA TGTCCCTATG GTGAAGGAAG
2941TCGGCTTGGC CCTGAGGACA TTATTGGCCA CTGTGGATGA GACCATTCCC CTCCTACCAG
3001CCAGCACCCA CCGAGAGATT GAGATGGCAC AGAAGCTATT GAACTCTGAC CTGGGTGAGC
3061TCATCAACAA GATGAAACTG GCCCAGCAGT ATGTCATGAC CAGCCTCCAG CAAGAGTACA
3121AAAAGCAAAT GCTGACTGCT GCTCACGCCC TGGCTGTGGA TGCCAAAAAC TTACTCGATG
3181TCATTGACCA AGCAAGACTG AAAATGCTTG GGCAGACGAG ACCACACTGA GACCCAGCTT
3241TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
3301CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
3361CTTGTGGAAA GGACGAAGGT TGAACTATAC ACTATACCTG ACGCGTTAAG TCgacaatca
3421acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)