Construct: ORF TRCN0000471115

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019016.3_s317c1
Derived from:
ccsbBroadEn_14818
DNA Barcode:
ACCCGTCAGACGGGGAGAATATCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PTK2 (5747)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471115
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5747 PTK2 protein tyrosine kinase 2 NM_001352694.2 100% 100%
2 human 5747 PTK2 protein tyrosine kinase 2 NM_153831.4 100% 100%
3 human 5747 PTK2 protein tyrosine kinase 2 NM_001352718.2 99.7% 99.7% 2709_2717delGCCATGGAG
4 human 5747 PTK2 protein tyrosine kinase 2 NM_001352719.2 99.7% 99.7% 2709_2717delGCCATGGAG
5 human 5747 PTK2 protein tyrosine kinase 2 NM_001352720.2 99.7% 99.7% 2709_2717delGCCATGGAG
6 human 5747 PTK2 protein tyrosine kinase 2 NM_001352717.2 99.4% 99.4% 1177_1194del
7 human 5747 PTK2 protein tyrosine kinase 2 XM_017013669.2 99.3% 99.2% 1235_1255del
8 human 5747 PTK2 protein tyrosine kinase 2 NM_001352715.2 99.1% 99.1% 1177_1194del;2727_2735delGCCATGGAG
9 human 5747 PTK2 protein tyrosine kinase 2 XM_017013664.2 99.1% 99.1% 1177_1194del;2727_2735delGCCATGGAG
10 human 5747 PTK2 protein tyrosine kinase 2 NM_001352713.2 99% 98.9% 1235_1255del;2730_2738delGCCATGGAG
11 human 5747 PTK2 protein tyrosine kinase 2 NM_001352714.2 99% 98.9% 1235_1255del;2730_2738delGCCATGGAG
12 human 5747 PTK2 protein tyrosine kinase 2 NM_001199649.2 98.7% 98.6% 2603_2632del;2739_2747delGCCATGGAG
13 human 5747 PTK2 protein tyrosine kinase 2 NM_001352706.2 98.5% 98.4% 1177_1194del;1253_1273del;2748_2756delGCCATGGAG
14 human 5747 PTK2 protein tyrosine kinase 2 NM_001352707.2 98.5% 98.4% 1177_1194del;1253_1273del;2748_2756delGCCATGGAG
15 human 5747 PTK2 protein tyrosine kinase 2 NM_001352708.2 98.5% 98.4% 1177_1194del;1253_1273del;2748_2756delGCCATGGAG
16 human 5747 PTK2 protein tyrosine kinase 2 NM_001352709.2 98.5% 98.4% 1177_1194del;1253_1273del;2748_2756delGCCATGGAG
17 human 5747 PTK2 protein tyrosine kinase 2 NM_001352710.2 98.5% 98.4% 1177_1194del;1253_1273del;2748_2756delGCCATGGAG
18 human 5747 PTK2 protein tyrosine kinase 2 NM_001352711.2 98.4% 98.3% (many diffs)
19 human 5747 PTK2 protein tyrosine kinase 2 NM_005607.5 97.9% 97.9% 1_66del
20 human 5747 PTK2 protein tyrosine kinase 2 NM_001352725.2 97.7% 97.7% 2498_2499ins63;2646_2654delGCCATGGAG
21 human 5747 PTK2 protein tyrosine kinase 2 NM_001352705.2 97.3% 97.2% (many diffs)
22 human 5747 PTK2 protein tyrosine kinase 2 NM_001352704.2 96.6% 96.6% 1177_1278del;2811_2819delGCCATGGAG
23 human 5747 PTK2 protein tyrosine kinase 2 NM_001352728.2 96.5% 96.5% 867_868ins108
24 human 5747 PTK2 protein tyrosine kinase 2 NM_001352721.2 96.5% 96.4% (many diffs)
25 human 5747 PTK2 protein tyrosine kinase 2 NM_001352722.2 96.5% 96.4% (many diffs)
26 human 5747 PTK2 protein tyrosine kinase 2 NM_001352723.2 96.5% 96.4% (many diffs)
27 human 5747 PTK2 protein tyrosine kinase 2 NM_001352712.2 96.2% 96.1% 1177_1278del;1337_1357del
28 human 5747 PTK2 protein tyrosine kinase 2 NM_001352699.2 95.9% 95.8% 1177_1278del;1337_1357del;2832_2840delGCCATGGAG
29 human 5747 PTK2 protein tyrosine kinase 2 NM_001352700.2 95.9% 95.8% 1177_1278del;1337_1357del;2832_2840delGCCATGGAG
30 human 5747 PTK2 protein tyrosine kinase 2 NM_001352701.2 95.9% 95.8% 1177_1278del;1337_1357del;2832_2840delGCCATGGAG
31 human 5747 PTK2 protein tyrosine kinase 2 NM_001352702.2 95.9% 95.8% 1177_1278del;1337_1357del;2832_2840delGCCATGGAG
32 human 5747 PTK2 protein tyrosine kinase 2 NM_001352703.2 95.9% 95.8% 1177_1278del;1337_1357del;2832_2840delGCCATGGAG
33 human 5747 PTK2 protein tyrosine kinase 2 NM_001352724.2 95.9% 95.8% (many diffs)
34 human 5747 PTK2 protein tyrosine kinase 2 XM_017013657.2 95.7% 95.7% 1_132del;2841_2849delGCCATGGAG
35 human 5747 PTK2 protein tyrosine kinase 2 XM_024447205.1 95.3% 95.3% 1_153del
36 human 5747 PTK2 protein tyrosine kinase 2 XM_017013656.2 95.3% 95.2% 1_132del;1367_1387del
37 human 5747 PTK2 protein tyrosine kinase 2 NM_001352726.2 95.1% 95% (many diffs)
38 human 5747 PTK2 protein tyrosine kinase 2 XM_024447211.1 95.1% 95% (many diffs)
39 human 5747 PTK2 protein tyrosine kinase 2 XM_024447204.1 95.1% 95.1% 1_153del;2862_2870delGCCATGGAG
40 human 5747 PTK2 protein tyrosine kinase 2 XM_017013654.2 95.1% 95% 1_132del;1367_1387del;2862_2870delGCCATGGAG
41 human 5747 PTK2 protein tyrosine kinase 2 NM_001352695.2 94.9% 94.9% 1177_1278del;2600_2601ins63
42 human 5747 PTK2 protein tyrosine kinase 2 XM_024447203.1 94.8% 94.8% 1_153del;1330_1347del
43 human 5747 PTK2 protein tyrosine kinase 2 NM_001352698.2 94.8% 94.7% (many diffs)
44 human 5747 PTK2 protein tyrosine kinase 2 XM_024447202.1 94.7% 94.6% 1_153del;1388_1408del
45 human 5747 PTK2 protein tyrosine kinase 2 XM_024447201.1 94.6% 94.6% 1_153del;1330_1347del;2880_2888delGCCATGGAG
46 human 5747 PTK2 protein tyrosine kinase 2 XM_017013653.2 94.6% 94.5% (many diffs)
47 human 5747 PTK2 protein tyrosine kinase 2 XM_024447200.1 94.5% 94.4% 1_153del;1388_1408del;2883_2891delGCCATGGAG
48 human 5747 PTK2 protein tyrosine kinase 2 XM_024447199.1 94% 93.9% (many diffs)
49 human 5747 PTK2 protein tyrosine kinase 2 NM_001352727.2 94% 93.9% (many diffs)
50 human 5747 PTK2 protein tyrosine kinase 2 XM_024447207.1 93.2% 93.2% 1_153del;2651_2652ins63;2799_2807delGCCATGGAG
51 human 5747 PTK2 protein tyrosine kinase 2 NM_001352729.2 93.1% 93% (many diffs)
52 human 5747 PTK2 protein tyrosine kinase 2 XM_017013666.1 92.7% 92.7% 1_132del;999_1000ins108
53 human 5747 PTK2 protein tyrosine kinase 2 NM_001352716.2 92.7% 92.6% (many diffs)
54 human 5747 PTK2 protein tyrosine kinase 2 XM_024447206.1 92.1% 92% (many diffs)
55 human 5747 PTK2 protein tyrosine kinase 2 XM_024447209.1 91.2% 91.1% (many diffs)
56 human 5747 PTK2 protein tyrosine kinase 2 XM_024447208.1 90.7% 90.7% (many diffs)
57 human 5747 PTK2 protein tyrosine kinase 2 NM_001352697.2 90.6% 90.5% (many diffs)
58 human 5747 PTK2 protein tyrosine kinase 2 NM_001352736.2 89.6% 89.6% 0_1ins303;874_891del;2424_2432delGCCATGGAG
59 human 5747 PTK2 protein tyrosine kinase 2 NM_001352741.2 89% 88.4% 0_1ins172;23_24ins167;2370_2378delGCCATGGAG
60 human 5747 PTK2 protein tyrosine kinase 2 XM_024447212.1 89% 88.4% 0_1ins172;23_24ins167;2370_2378delGCCATGGAG
61 human 5747 PTK2 protein tyrosine kinase 2 XM_024447210.1 88.9% 88.8% (many diffs)
62 human 5747 PTK2 protein tyrosine kinase 2 NM_001352740.2 88.5% 87.9% (many diffs)
63 human 5747 PTK2 protein tyrosine kinase 2 NM_001352739.2 88.4% 87.7% (many diffs)
64 human 5747 PTK2 protein tyrosine kinase 2 XM_017013684.2 88.4% 87.7% (many diffs)
65 human 5747 PTK2 protein tyrosine kinase 2 NM_001352738.2 87.9% 87.2% (many diffs)
66 human 5747 PTK2 protein tyrosine kinase 2 NM_001316342.2 87.9% 87.2% (many diffs)
67 human 5747 PTK2 protein tyrosine kinase 2 NM_001352696.2 87.9% 87.2% (many diffs)
68 human 5747 PTK2 protein tyrosine kinase 2 NM_001352737.2 86.4% 85.9% 0_1ins172;23_24ins167;838_939del
69 human 5747 PTK2 protein tyrosine kinase 2 NM_001352733.2 86.2% 85.6% (many diffs)
70 human 5747 PTK2 protein tyrosine kinase 2 NM_001352742.2 85.9% 85.2% (many diffs)
71 human 5747 PTK2 protein tyrosine kinase 2 NM_001352743.2 85.9% 85.2% (many diffs)
72 human 5747 PTK2 protein tyrosine kinase 2 NM_001352732.2 85.9% 85.2% (many diffs)
73 human 5747 PTK2 protein tyrosine kinase 2 NM_001352731.2 85.7% 85% (many diffs)
74 human 5747 PTK2 protein tyrosine kinase 2 NM_001352730.2 85.6% 85% (many diffs)
75 human 5747 PTK2 protein tyrosine kinase 2 NM_001352745.2 85% 84.3% (many diffs)
76 human 5747 PTK2 protein tyrosine kinase 2 NM_001352744.2 84.5% 83.8% (many diffs)
77 human 5747 PTK2 protein tyrosine kinase 2 XM_017013688.2 84.5% 83.8% (many diffs)
78 human 5747 PTK2 protein tyrosine kinase 2 NM_001352734.2 83.7% 83.1% (many diffs)
79 human 5747 PTK2 protein tyrosine kinase 2 NM_001352735.2 83.7% 83.1% (many diffs)
80 human 5747 PTK2 protein tyrosine kinase 2 NM_001352746.2 82.5% 81.9% (many diffs)
81 human 5747 PTK2 protein tyrosine kinase 2 NR_148036.2 59.2% (many diffs)
82 human 5747 PTK2 protein tyrosine kinase 2 NR_148037.2 58.9% (many diffs)
83 human 5747 PTK2 protein tyrosine kinase 2 NR_148038.2 56.9% (many diffs)
84 human 5747 PTK2 protein tyrosine kinase 2 NR_148039.2 54.8% (many diffs)
85 human 5747 PTK2 protein tyrosine kinase 2 NM_001352747.2 54.7% 54.7% 0_1ins1422;1287_1295delGCCATGGAG
86 human 5747 PTK2 protein tyrosine kinase 2 NM_001352748.2 45.6% 44.3% (many diffs)
87 human 5747 PTK2 protein tyrosine kinase 2 NM_001352750.2 34.4% 34.4% 0_1ins2070
88 human 5747 PTK2 protein tyrosine kinase 2 NM_001352749.2 34.3% 34.3% 0_1ins2070;639_647delGCCATGGAG
89 human 5747 PTK2 protein tyrosine kinase 2 NM_001352752.2 32.4% 32.4% 0_1ins2070;428_429ins63
90 human 5747 PTK2 protein tyrosine kinase 2 NM_001352751.2 32.3% 32.3% 0_1ins2070;428_429ins63;576_584delGCCATGGAG
91 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 NM_007982.2 90.7% 97.3% (many diffs)
92 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520433.2 90.5% 97% (many diffs)
93 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_017316429.1 89.4% 95.7% (many diffs)
94 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520432.2 87.1% 93.4% (many diffs)
95 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520424.2 87.1% 93.3% (many diffs)
96 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520425.2 87.1% 93.3% (many diffs)
97 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520426.2 87.1% 93.3% (many diffs)
98 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520427.3 87.1% 93.3% (many diffs)
99 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520428.2 87.1% 93.3% (many diffs)
100 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520429.2 87.1% 93.3% (many diffs)
101 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520430.2 87.1% 93.3% (many diffs)
102 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520431.2 87.1% 93.3% (many diffs)
103 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520435.3 87.1% 93.3% (many diffs)
104 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520422.1 86.9% 93.1% (many diffs)
105 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520423.1 86.9% 93.1% (many diffs)
106 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_017316428.1 86.5% 92.7% (many diffs)
107 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520421.1 86.3% 92.4% (many diffs)
108 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520420.1 85.8% 91.9% (many diffs)
109 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520418.1 84.2% 90.3% (many diffs)
110 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_011245452.2 83.9% 89.9% (many diffs)
111 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520415.2 83.7% 89.7% (many diffs)
112 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520416.2 83.7% 89.7% (many diffs)
113 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_011245453.1 82.1% 87.9% (many diffs)
114 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 NM_001130409.1 79.2% 82.7% (many diffs)
115 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XM_006520434.2 78.3% 84.3% (many diffs)
116 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XR_383981.2 50.4% (many diffs)
117 mouse 14083 Ptk2 PTK2 protein tyrosine kinase 2 XR_001781493.1 32.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3225
ORF length:
3156
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcagctgct taccttgacc ccaacttgaa tcacacacca aattcgagta
121ctaagactca cctgggtact ggtatggaac gttctcctgg tgcaatggag cgagtattaa
181aggtctttca ttattttgaa agcaatagtg agccaaccac ctgggccagt attatcaggc
241atggagatgc tactgatgtc aggggcatca ttcagaagat agtggacagt cacaaagtaa
301agcatgtggc ctgctatgga ttccgcctca gtcacctgcg gtcagaggag gttcactggc
361ttcacgtgga tatgggcgtc tccagtgtga gggagaagta tgagcttgct cacccaccag
421aggagtggaa atatgaattg agaattcgtt atttgccaaa aggatttcta aaccagttta
481ctgaagataa gccaactttg aatttcttct atcaacaggt gaagagcgat tatatgttag
541agatagctga tcaagtggac caggaaattg ctttgaagtt gggttgtcta gaaatacggc
601gatcatactg ggagatgcgg ggcaatgcac tagaaaagaa gtctaactat gaagtattag
661aaaaagatgt tggtttaaag cgattttttc ctaagagttt actggattct gtcaaggcca
721aaacactaag aaaactgatc caacaaacat ttagacaatt tgccaacctt aatagagaag
781aaagtattct gaaattcttt gagatcctgt ctccagtcta cagatttgat aaggaatgct
841tcaagtgtgc tcttggttca agctggatta tttcagtgga actggcaatc ggcccagaag
901aaggaatcag ttacctaacg gacaagggct gcaatcccac acatcttgct gacttcactc
961aagtgcaaac cattcagtat tcaaacagtg aagacaagga cagaaaagga atgctacaac
1021taaaaatagc aggtgcaccc gagcctctga cagtgacggc accatcccta accattgcgg
1081agaatatggc tgacctaata gatgggtact gccggctggt gaatggaacc tcgcagtcat
1141ttatcatcag acctcagaaa gaaggtgaac gggctttgcc atcaatacca aagttggcca
1201acagcgaaaa gcaaggcatg cggacacacg ccgtctctgt gtcagaaaca gatgattatg
1261ctgagattat agatgaagaa gatacttaca ccatgccctc aaccagggat tatgagattc
1321aaagagaaag aatagaactt ggacgatgta ttggagaagg ccaatttgga gatgtacatc
1381aaggcattta tatgagtcca gagaatccag ctttggcggt tgcaattaaa acatgtaaaa
1441actgtacttc ggacagcgtg agagagaaat ttcttcaaga agccttaaca atgcgtcagt
1501ttgaccatcc tcatattgtg aagctgattg gagtcatcac agagaatcct gtctggataa
1561tcatggagct gtgcacactt ggagagctga ggtcattttt gcaagtaagg aaatacagtt
1621tggatctagc atctttgatc ctgtatgcct atcagcttag tacagctctt gcatatctag
1681agagcaaaag atttgtacac agggacattg ctgctcggaa tgttctggtg tcctcaaatg
1741attgtgtaaa attaggagac tttggattat cccgatatat ggaagatagt acttactaca
1801aagcttccaa aggaaaattg cctattaaat ggatggctcc agagtcaatc aattttcgac
1861gttttacctc agctagtgac gtatggatgt ttggtgtgtg tatgtgggag atactgatgc
1921atggtgtgaa gccttttcaa ggagtgaaga acaatgatgt aatcggtcga attgaaaatg
1981gggaaagatt accaatgcct ccaaattgtc ctcctaccct ctacagcctt atgacgaaat
2041gctgggccta tgaccccagc aggcggccca ggtttactga acttaaagct cagctcagca
2101caatcctgga ggaagagaag gctcagcaag aagagcgcat gaggatggag tccagaagac
2161aggccacagt gtcctgggac tccggagggt ctgatgaagc accgcccaag cccagcagac
2221cgggttatcc cagtccgagg tccagcgaag gattttatcc cagcccacag cacatggtac
2281aaaccaatca ttaccaggtt tctggctacc ctggttcaca tggaatcaca gccatggctg
2341gcagcatcta tccaggtcag gcatctcttt tggaccaaac agattcatgg aatcatagac
2401ctcaggagat agcaatgtgg cagcccaatg tggaggactc tacagtattg gacctgcgag
2461ggattgggca agtgttgcca acccatctga tggaagagcg tctaatccga cagcaacagg
2521aaatggaaga agatcagcgc tggctggaaa aagaggaaag atttctgaaa cctgatgtga
2581gactctctcg aggcagtatt gacagggagg atggaagtct tcagggtccg attggaaacc
2641aacatatata tcagcctgtg ggtaaaccag atcctgcagc tccaccaaag aaaccgcctc
2701gccctggagc tcccggtcat ctgggaagcc ttgccagcct cagcagccct gctgacagct
2761acaacgaggg tgtcaagctt cagccccagg aaatcagccc ccctcctact gccaaccTGG
2821ACCGGTCGAA TGATAAGGTG TACGAGAATG TGACGGGCCT GGTGAAAGCT GTCATCGAGA
2881TGTCCAGTAA AATCCAGCCA GCCCCACCAG AGGAGTATGT CCCTATGGTG AAGGAAGTCG
2941GCTTGGCCCT GAGGACATTA TTGGCCACTG TGGATGAGAC CATTCCCCTC CTACCAGCCA
3001GCACCCACCG AGAGATTGAG ATGGCACAGA AGCTATTGAA CTCTGACCTG GGTGAGCTCA
3061TCAACAAGAT GAAACTGGCC CAGCAGTATG TCATGACCAG CCTCCAGCAA GAGTACAAAA
3121AGCAAATGCT GACTGCTGCT CACGCCCTGG CTGTGGATGC CAAAAACTTA CTCGATGTCA
3181TTGACCAAGC AAGACTGAAA ATGCTTGGGC AGACGAGACC ACACCCAACT TTCTTGTACA
3241AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
3301AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
3361AGGACGAACC CGTCAGACGG GGAGAATATC AACGCGTTAA GTCgacaatc aacctctgga
3421ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)