Transcript: Human NM_001354061.2

Homo sapiens Rho guanine nucleotide exchange factor 7 (ARHGEF7), transcript variant 27, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
ARHGEF7 (8874)
Length:
4672
CDS:
307..1812

Additional Resources:

NCBI RefSeq record:
NM_001354061.2
NBCI Gene record:
ARHGEF7 (8874)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001354061.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000047594 GCGGATATTAGTGTCGTGCAA pLKO.1 1062 CDS 100% 2.640 3.696 N ARHGEF7 n/a
2 TRCN0000047597 CTCTGCTACAAGGAGGATCTT pLKO.1 1399 CDS 100% 4.950 3.960 N ARHGEF7 n/a
3 TRCN0000303860 AGAGACACATGGAGGATTATC pLKO_005 662 CDS 100% 13.200 9.240 N ARHGEF7 n/a
4 TRCN0000305803 AGAGACACATGGAGGATTATC pLKO_005 662 CDS 100% 13.200 9.240 N Arhgef7 n/a
5 TRCN0000047596 GAAGTTAAGTTCAGCAAACAT pLKO.1 256 5UTR 100% 5.625 3.938 N ARHGEF7 n/a
6 TRCN0000300058 GAAGTTAAGTTCAGCAAACAT pLKO_005 256 5UTR 100% 5.625 3.938 N ARHGEF7 n/a
7 TRCN0000303775 ACTGGGAGGGCGATGACATTA pLKO_005 803 CDS 100% 13.200 7.920 N ARHGEF7 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001354061.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07317 pDONR223 100% 62.1% 61.6% None (many diffs) n/a
2 ccsbBroad304_07317 pLX_304 0% 62.1% 61.6% V5 (many diffs) n/a
3 TRCN0000474600 ATGTATAGCGCCCTCCCCCCGCCC pLX_317 16.8% 62.1% 61.6% V5 (many diffs) n/a
4 ccsbBroadEn_02036 pDONR223 100% 58.5% 58.1% None 0_1ins965;49_107del n/a
5 ccsbBroad304_02036 pLX_304 0% 58.5% 58.1% V5 0_1ins965;49_107del n/a
6 TRCN0000479549 CAGCCCGAGTTGTATTTCACCCGA pLX_317 16% 58.5% 58.1% V5 0_1ins965;49_107del n/a
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