Transcript: Mouse NM_001363463.1

Mus musculus Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase (Sepsecs), transcript variant 2, mRNA.

Source:
NCBI, updated 2018-09-30
Taxon:
Mus musculus (mouse)
Gene:
Sepsecs (211006)
Length:
5176
CDS:
370..1461

Additional Resources:

NCBI RefSeq record:
NM_001363463.1
NBCI Gene record:
Sepsecs (211006)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001363463.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000248930 CCAGTAGGTGGTGCTATAATT pLKO_005 811 CDS 100% 15.000 21.000 N Sepsecs n/a
2 TRCN0000425956 CCAGTAGGTGGTGCTATAATT pLKO_005 811 CDS 100% 15.000 21.000 N SEPSECS n/a
3 TRCN0000257819 TTGGACGATCCGGTGATATTT pLKO_005 230 5UTR 100% 15.000 21.000 N Sepsecs n/a
4 TRCN0000248929 CAAAGGCCAAGTATATCATAT pLKO_005 425 CDS 100% 13.200 9.240 N Sepsecs n/a
5 TRCN0000191321 CCTCAGATGATGCTTACTAAT pLKO.1 1584 3UTR 100% 13.200 9.240 N Sepsecs n/a
6 TRCN0000257810 TCAGCATTCAGAGGTTGTTTG pLKO_005 1664 3UTR 100% 10.800 7.560 N Sepsecs n/a
7 TRCN0000189702 GCAGGTGATTCTCACTCAGAT pLKO.1 1620 3UTR 100% 4.950 3.465 N Sepsecs n/a
8 TRCN0000127512 CGGTGATATTTCTGCTGTGCA pLKO.1 240 5UTR 100% 2.640 1.848 N SEPSECS n/a
9 TRCN0000433478 GCTGTGATTTGTGCTAATTAT pLKO_005 661 CDS 100% 15.000 9.000 N SEPSECS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001363463.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11945 pDONR223 100% 69.1% 69.5% None (many diffs) n/a
2 ccsbBroad304_11945 pLX_304 0% 69.1% 69.5% V5 (many diffs) n/a
3 ccsbBroadEn_11946 pDONR223 100% 61.1% 61.3% None (many diffs) n/a
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