Construct: ORF ccsbBroadEn_11946
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011013.1_s300c1, BRDN0000398331
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEPSECS (51091)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | XM_011513846.2 | 100% | 100% | |
| 2 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | XM_011513847.2 | 95.1% | 92.4% | (many diffs) |
| 3 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | NM_016955.4 | 93.6% | 92.3% | (many diffs) |
| 4 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | XM_011513848.1 | 88.2% | 88.2% | 0_1ins177 |
| 5 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | XM_017008277.1 | 85.3% | 78.3% | 1_140del;252_369del |
| 6 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | XM_017008278.1 | 72% | 72% | 0_1ins420 |
| 7 | mouse | 211006 | Sepsecs | Sep (O-phosphoserine) tRNA:... | NM_172490.4 | 81.6% | 79.2% | (many diffs) |
| 8 | mouse | 211006 | Sepsecs | Sep (O-phosphoserine) tRNA:... | NM_001363463.1 | 61.1% | 61.3% | (many diffs) |
| 9 | mouse | 211006 | Sepsecs | Sep (O-phosphoserine) tRNA:... | XM_006503827.4 | 44.8% | 43.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1569
- ORF length:
- 1500
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaacttTG TACAAGAAAG 61 TTGGCACCAT GCAGTGTGAC GATCTCGGCT CACTGCAGCC TCCGCCTCCC GGGTTCACGC 121 CATTCGCCTG CCTCAGCCTC CCGAGTAGCT GGGACTACAG GCGCCCGCCA CCACACCCGG 181 GCAAGTGTCC AGAGAATGGC TGGGATGAAA GTACACTTGA ACTCTTTTTA CATGAACTTG 241 CAATCATGGA CAGCAACAAT TTCTTAGGCA ATTGTGGTGT GGGAGAAAGG GAAGGGAGAG 301 TGGCATCCGC ACTGGTTGCT CGTCGTCATT ACAGGTTCAT TCATGGCATT GGACGATCCG 361 GTGATATTTC TGCTGTGCAA CCAAAAGCTG CAGGCTCTAG CCTTTTGAAC AAAATTACCA 421 ATTCTTTGGT CCTGGACATT ATAAAGCTGG CTGGTGTCCA TACAGTAGCC AACTGCTTTG 481 TAGTTCCTAT GGCAACTGGT ATGAGTCTAA CTCTGTGTTT CTTAACATTA CGACACAAAA 541 GACCAAAGGC AAAGTATATT ATATGGCCAC GAATAGACCA GAAGTCCTGC TTTAAATCCA 601 TGATCACTGC AGGTTTTGAG CCTGTGGTGA TAGAAAATGT TTTGGAAGGT GACGAGCTGC 661 GTACAGACCT GAAAGCAGTG GAGGCTAAAG TCCAGGAACT TGGGCCTGAT TGCATTCTGT 721 GTATTCATTC TACTACATCC TGTTTTGCTC CAAGGGTGCC TGATAGATTA GAAGAACTGG 781 CTGTGATTTG TGCTAATTAT GACATTCCAC ATATAGTTAA TAATGCTTAT GGAGTGCAGT 841 CTTCAAAGTG TATGCATCTC ATTCAGCAGG GGGCTCGAGT TGGTAGAATA GATGCTTTTG 901 TTCAGAGCTT GGACAAAAAT TTTATGGTTC CAGTAGGTGG TGCTATAATT GCTGGCTTTA 961 ATGATTCATT CATTCAGGAA ATCAGCAAGA TGTATCCAGG AAGAGCTTCA GCTTCACCTT 1021 CTTTAGATGT CCTTATTACT TTATTGTCAC TTGGATCAAA TGGCTATAAG AAGCTACTAA 1081 AAGAAAGAAA GGAAATGTTT TCATATTTGT CCAACCAAAT AAAGAAGTTG TCAGAAGCCT 1141 ACAATGAAAG ACTGTTGCAT ACACCTCACA ATCCCATATC TTTAGCTATG ACACTTAAAA 1201 CACTAGATGA ACACCGTGAC AAAGCTGTCA CTCAGCTTGG CTCGATGCTT TTTACCAGAC 1261 AGGTTTCTGG AGCCAGGGTT GTGCCTCTTG GGTCCATGCA AACTGTGAGT GGCTATACTT 1321 TCAGAGGCTT TATGTCACAT ACAAATAATT ACCCTTGTGC TTACCTCAAT GCTGCATCAG 1381 CCATCGGAAT GAAGATGCAG GATGTGGACC TGTTCATAAA GAGACTTGAC AGGTGTTTAA 1441 AGGCAGTAAG AAAAGAACGA AGTAAAGAGA GTGATGACAA TTATGACAAA ACTGAAGATG 1501 TGGATATTGA AGAAATGGCT TTAAAACTAG ATAATGTACT TCTTGACACA TACCAGGATG 1561 CTTCTTCATT GCCAACTTTC TTGTACAAAG Ttggcattat aagaaagcat tgcttatcaa 1621 tttgttgcaa cgaac