Construct: ORF ccsbBroad304_11945
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002848.1_s304c1, BRDN0000405128
- Derived from:
- ccsbBroadEn_11945
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SEPSECS (51091)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_11945
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | XM_011513848.1 | 100% | 100% | |
| 2 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | XM_011513847.2 | 90% | 90% | 1_147del |
| 3 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | XM_011513846.2 | 88.2% | 88.2% | 1_177del |
| 4 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | NM_016955.4 | 88% | 88% | 1_180del |
| 5 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | XM_017008278.1 | 81.6% | 81.6% | 0_1ins243 |
| 6 | human | 51091 | SEPSECS | Sep (O-phosphoserine) tRNA:... | XM_017008277.1 | 75.2% | 75.2% | 1_435del |
| 7 | mouse | 211006 | Sepsecs | Sep (O-phosphoserine) tRNA:... | NM_172490.4 | 75.2% | 76.4% | (many diffs) |
| 8 | mouse | 211006 | Sepsecs | Sep (O-phosphoserine) tRNA:... | NM_001363463.1 | 69.1% | 69.5% | (many diffs) |
| 9 | mouse | 211006 | Sepsecs | Sep (O-phosphoserine) tRNA:... | XM_006503827.4 | 37.7% | 39.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1392
- ORF length:
- 1323
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacagcaac aatttcttag gcaattgtgg tgtgggagaa agggaaggga 121 gagtggcatc cgcactggtt gctcgtcgtc attacaggtt cattcatggc attggacgat 181 ccggtgatat ttctgctgtg caaccaaaag ctgcaggctc tagccttttg aacaaaatta 241 ccaattcttt ggtcctggac attataaagc tggctggtgt ccatacagta gccaactgct 301 ttgtagttcc tatggcaact ggtatgagtc taactctgtg tttcttaaca ttacgacaca 361 aaagaccaaa ggcaaagtat attatatggc cacgaataga ccagaagtcc tgctttaaat 421 ccatgatcac tgcaggtttt gagcctgtgg tgatagaaaa tgttttggaa ggtgacgagc 481 tgcgtacaga cctgaaagca gtggaggcta aagtccagga acttgggcct gattgcattc 541 tgtgtattca ttctactaca tcctgttttg ctccaagggt gcctgataga ttagaagaac 601 tggctgtgat ttgtgctaat tatgacattc cacatatagt taataatgct tatggagtgc 661 agtcttcaaa gtgtatgcat ctcattcagc agggggctcg agttggtaga atagatgctt 721 ttgttcagag cttggacaaa aattttatgg ttccagtagg tggtgctata attgctggct 781 ttaatgattc attcattcag gaaatcagca agatgtatcc aggaagagct tcagcttcac 841 cttctttaga tgtccttatt actttattgt cacttggatc aaatggctat aagaagctac 901 taaaagaaag aaaggaaatg ttttcatatt tgtccaacca aataaagaag ttgtcagaag 961 cctacaatga aagactgttg catacacctc acaatcccat atctttagct atgacactta 1021 aaacactaga tgaacaccgt gacaaagctg tcactcagct tggctcgatg ctttttacca 1081 gacaggtttc tggagccagg gttgtgcctc ttgggtccat gcaaactgtg agtggctata 1141 ctttcagagg ctttatgtca catacaaata attacccttg tgcttacctc aatgctgcat 1201 cagccatcgg aatgaagatg caggatgtgg acctgttcat aaagagactt gacaggtgtt 1261 taaaggcagt aagaaaagaa cgaagtaaag agagtgatga caattatgac aaaactgaag 1321 atgtggatat tgaagaaatg gctttaaaac tagataatgt acttcttgac acataccagg 1381 atgcttcttc attgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 1441 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 1501 caatcaacct ctggatta