Transcript: Human NM_001364149.2

Homo sapiens casein kinase 1 gamma 3 (CSNK1G3), transcript variant 16, mRNA.

Source:
NCBI, updated 2019-08-09
Taxon:
Homo sapiens (human)
Gene:
CSNK1G3 (1456)
Length:
4100
CDS:
401..1447

Additional Resources:

NCBI RefSeq record:
NM_001364149.2
NBCI Gene record:
CSNK1G3 (1456)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145821 AGGCTGACACATTAAAGGAG pXPR_003 AGG 549 52% 6 0.4292 CSNK1G3 CSNK1G3 77361
2 BRDN0001145081 ATAGCATTGTATTTACCACA pXPR_003 AGG 99 9% 3 0.206 CSNK1G3 CSNK1G3 77362
3 BRDN0001144972 AACTTCTTAATAGGACGACC pXPR_003 AGG 290 28% 4 -0.0622 CSNK1G3 CSNK1G3 77363
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001364149.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000355670 TTTCGGCCCTTGTGGTAAATA pLKO_005 487 CDS 100% 15.000 21.000 N CSNK1G3 n/a
2 TRCN0000023764 CCAGGTTGTAAGTTCTACAAA pLKO.1 1258 CDS 100% 0.563 0.788 N Csnk1g3 n/a
3 TRCN0000196591 GCAACATATCTTCGTTATGTA pLKO.1 1025 CDS 100% 0.563 0.788 N CSNK1G3 n/a
4 TRCN0000338228 CAAAGTAGAAGAGACGATTTA pLKO_005 851 CDS 100% 13.200 10.560 N CSNK1G3 n/a
5 TRCN0000010550 CCCAGCAAGTTATTCACATTA pLKO.1 705 CDS 100% 13.200 10.560 N CSNK1G3 n/a
6 TRCN0000196897 GCTAGATATATGAGCATAAAC pLKO.1 812 CDS 100% 13.200 9.240 N CSNK1G3 n/a
7 TRCN0000196403 GTGATGTATGTTGGAGTTATA pLKO.1 2263 3UTR 100% 13.200 9.240 N CSNK1G3 n/a
8 TRCN0000355669 TTTCCAGAAATGGCAACATAT pLKO_005 1013 CDS 100% 13.200 9.240 N CSNK1G3 n/a
9 TRCN0000420971 TAGGATCTGGAGATGGTATAC pLKO_005 453 CDS 100% 10.800 7.560 N Csnk1g3 n/a
10 TRCN0000000808 CACCGAAACTTACTGCTGAAT pLKO.1 2357 3UTR 100% 4.950 3.465 N CSNK1G3 n/a
11 TRCN0000338227 CACCGAAACTTACTGCTGAAT pLKO_005 2357 3UTR 100% 4.950 3.465 N CSNK1G3 n/a
12 TRCN0000195537 CCCTACTGAAGTAGAAGTGAT pLKO.1 1336 CDS 100% 4.950 3.465 N CSNK1G3 n/a
13 TRCN0000000809 CCGGAGACAAAGAAACACATA pLKO.1 758 CDS 100% 4.950 3.465 N CSNK1G3 n/a
14 TRCN0000338167 CCGGAGACAAAGAAACACATA pLKO_005 758 CDS 100% 4.950 3.465 N CSNK1G3 n/a
15 TRCN0000000810 AGGACGTTCAAATGCACCCAT pLKO.1 1309 CDS 100% 2.640 1.848 N CSNK1G3 n/a
16 TRCN0000000811 ACTTCTTAATAGGACGACCAA pLKO.1 675 CDS 100% 2.640 3.696 N CSNK1G3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001364149.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06057 pDONR223 100% 82.1% 82% None 0_1ins225;46A>G n/a
2 ccsbBroad304_06057 pLX_304 0% 82.1% 82% V5 0_1ins225;46A>G n/a
3 TRCN0000477806 CGATTTGCAATTGATGCTTGGCAT pLX_317 26.7% 82.1% 82% V5 0_1ins225;46A>G n/a
4 ccsbBroadEn_14603 pDONR223 100% 82% 81.5% None (many diffs) n/a
5 ccsbBroad304_14603 pLX_304 0% 82% 81.5% V5 (many diffs) n/a
6 TRCN0000467288 CGAATTGCCCCTTACTCTATAGGA pLX_317 11.6% 82% 81.5% V5 (many diffs) n/a
7 TRCN0000489732 TAATGTCAATGTAAAACACCATGT pLX_317 37.4% 48.2% 32.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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