Transcript: Human NM_001369913.1

Homo sapiens SH3 domain containing GRB2 like, endophilin B2 (SH3GLB2), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-07-07
Taxon:
Homo sapiens (human)
Gene:
SH3GLB2 (56904)
Length:
2813
CDS:
674..1921

Additional Resources:

NCBI RefSeq record:
NM_001369913.1
NBCI Gene record:
SH3GLB2 (56904)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001369913.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000323061 GGAGACTAGACCTCGTAATTA pLKO_005 1297 CDS 100% 15.000 21.000 N SH3GLB2 n/a
2 TRCN0000093572 AGGAGACTAGACCTCGTAATT pLKO.1 1296 CDS 100% 13.200 18.480 N Sh3glb2 n/a
3 TRCN0000093571 TCAGGAGACTAGACCTCGTAA pLKO.1 1294 CDS 100% 4.950 6.930 N Sh3glb2 n/a
4 TRCN0000324461 TCAGGAGACTAGACCTCGTAA pLKO_005 1294 CDS 100% 4.950 6.930 N Sh3glb2 n/a
5 TRCN0000160989 GTAATTACATTCTCTCGGCCA pLKO.1 1311 CDS 100% 0.540 0.756 N SH3GLB2 n/a
6 TRCN0000271569 CAAGGTCCCTGTCACCTACTT pLKO_005 1885 CDS 100% 4.950 3.960 N SH3GLB2 n/a
7 TRCN0000162304 CAAGTCTCAGACAACCTACTA pLKO.1 1489 CDS 100% 4.950 3.960 N SH3GLB2 n/a
8 TRCN0000271617 ATCAGTAGCACTCACGTGAAC pLKO_005 1442 CDS 100% 4.050 3.240 N SH3GLB2 n/a
9 TRCN0000323062 CTGAGCTTGATGCCCACTTTG pLKO_005 804 CDS 100% 10.800 7.560 N SH3GLB2 n/a
10 TRCN0000165368 GAGCTTGATGCCCACTTTGAA pLKO.1 806 CDS 100% 5.625 3.938 N SH3GLB2 n/a
11 TRCN0000284481 AGCAGCATCTTCCGGTGCTAT pLKO_005 2412 3UTR 100% 4.950 3.465 N SH3GLB2 n/a
12 TRCN0000165782 CACCAAGAACTGGACAGAGAA pLKO.1 850 CDS 100% 4.950 3.465 N SH3GLB2 n/a
13 TRCN0000166673 CCTGACTTTCAGGAGACTAGA pLKO.1 1286 CDS 100% 4.950 3.465 N SH3GLB2 n/a
14 TRCN0000166184 CGTCAAGTCTCAGACAACCTA pLKO.1 1486 CDS 100% 3.000 2.100 N SH3GLB2 n/a
15 TRCN0000160383 CCACTTAACTTGTTTGTTGGT pLKO.1 1982 3UTR 100% 2.640 1.848 N SH3GLB2 n/a
16 TRCN0000162583 CTATGGGAAGACACTGATCAA pLKO.1 1036 CDS 100% 4.950 2.970 N SH3GLB2 n/a
17 TRCN0000162512 CTGGAATGATGAAGTGGACAA pLKO.1 1345 CDS 100% 4.050 2.430 N SH3GLB2 n/a
18 TRCN0000380406 AGTCTCAGACAACCTACTATG pLKO_005 1491 CDS 100% 10.800 7.560 N Sh3glb2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001369913.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03753 pDONR223 100% 92.6% 90.6% None (many diffs) n/a
2 ccsbBroad304_03753 pLX_304 0% 92.6% 90.6% V5 (many diffs) n/a
3 TRCN0000469605 TACACTTGGTCCCTTGTGCAGTAC pLX_317 28.5% 92.6% 90.6% V5 (many diffs) n/a
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