Construct: ORF TRCN0000469605
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000655.1_s317c1
- Derived from:
- ccsbBroadEn_03753
- DNA Barcode:
- TACACTTGGTCCCTTGTGCAGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SH3GLB2 (56904)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469605
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | NM_001287046.1 | 99.9% | 100% | 960G>A |
| 2 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | NM_020145.3 | 99.9% | 100% | 960G>A |
| 3 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | XM_006717191.1 | 98.9% | 98.9% | 562_573del;972G>A |
| 4 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | XM_005252098.2 | 98.6% | 98.5% | 840_854del;975G>A |
| 5 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | NM_001287045.1 | 97.6% | 97.5% | 562_573del;852_866del;987G>A |
| 6 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | NM_001369915.1 | 93.4% | 93.2% | 559_560ins63;777_791del;912G>A |
| 7 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | XM_006717190.1 | 93.3% | 93.3% | 562_573del;636_707del;1044G>A |
| 8 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | XM_006717189.2 | 93% | 92.9% | 624_695del;912_926del;1047G>A |
| 9 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | NM_001369913.1 | 92.6% | 90.6% | (many diffs) |
| 10 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | XM_006717188.2 | 92.2% | 92% | (many diffs) |
| 11 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | NM_001369914.1 | 70.5% | 74% | 562_573del;849_850ins241;957_1098del |
| 12 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | NR_163192.1 | 46.2% | (many diffs) | |
| 13 | human | 56904 | SH3GLB2 | SH3 domain containing GRB2 ... | XM_005252101.3 | 31.5% | 31.6% | 0_1ins810;150G>A |
| 14 | mouse | 227700 | Sh3glb2 | SH3-domain GRB2-like endoph... | NM_139302.2 | 90.5% | 96.9% | (many diffs) |
| 15 | mouse | 227700 | Sh3glb2 | SH3-domain GRB2-like endoph... | XM_006497937.1 | 89.6% | 95.9% | (many diffs) |
| 16 | mouse | 227700 | Sh3glb2 | SH3-domain GRB2-like endoph... | NM_001289709.1 | 89.4% | 95.5% | (many diffs) |
| 17 | mouse | 227700 | Sh3glb2 | SH3-domain GRB2-like endoph... | NM_001346806.1 | 88.5% | 94.5% | (many diffs) |
| 18 | mouse | 227700 | Sh3glb2 | SH3-domain GRB2-like endoph... | NM_001289710.1 | 85.3% | 91.6% | (many diffs) |
| 19 | mouse | 227700 | Sh3glb2 | SH3-domain GRB2-like endoph... | XM_006497938.1 | 84.7% | 90.5% | (many diffs) |
| 20 | mouse | 227700 | Sh3glb2 | SH3-domain GRB2-like endoph... | XM_006497939.1 | 84.2% | 90.2% | (many diffs) |
| 21 | mouse | 227700 | Sh3glb2 | SH3-domain GRB2-like endoph... | XM_011239078.1 | 75.7% | 69.8% | (many diffs) |
| 22 | mouse | 227700 | Sh3glb2 | SH3-domain GRB2-like endoph... | XM_011239077.1 | 74.8% | 64.7% | (many diffs) |
| 23 | mouse | 227700 | Sh3glb2 | SH3-domain GRB2-like endoph... | XM_006497941.3 | 66% | 70.7% | (many diffs) |
| 24 | mouse | 227700 | Sh3glb2 | SH3-domain GRB2-like endoph... | XM_006497942.3 | 66% | 70.7% | (many diffs) |
| 25 | mouse | 227700 | Sh3glb2 | SH3-domain GRB2-like endoph... | XR_001782060.1 | 36.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1251
- ORF length:
- 1185
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cttcaacatg aagaagctgg cgtcggacgc gggcatcttc ttcacccggg 121 cggtgcagtt cacggaggag aaatttggcc aggctgagaa gactgagctt gatgcccact 181 ttgaaaacct tctggcccgg gcagacagca ccaagaactg gacagagaag atcttgaggc 241 agacagaggt gctgctgcag cccaacccca gtgcccgagt ggaggagttc ctgtatgaga 301 agctggacag gaaggtcccc tcaagggtca ccaacgggga gctgctggct cagtacatgg 361 cagacgcggc cagtgagctg gggccgacca ccccctatgg gaagacactg atcaaggtgg 421 cagaagctga aaagcaactg ggagccgcgg agagggattt tatccacacg gcctccatca 481 gcttcctcac acccttgcgc aacttcctgg agggggactg gaagaccatc tcgaaggaga 541 ggcggctcct ccaaaaccgg cgtctggact tggatgcctg caaagcgagg ctgaagaagg 601 ccaaggctgc agaagccaaa gccacgacgg tgcctgactt tcaggagact agacctcgta 661 attacattct ctcggccagc gcctccgcgc tctggaatga tgaagtggac aaggccgagc 721 aggagctccg cgtggcccag acagagtttg accggcaagc agaagtgacc cgtctcttgc 781 tggagggaat cagtagcact cacgtgaacc acctgcgctg cctccacgag ttcgtcaagt 841 ctcagacaac ctactacgca cagtgctacc gccacatgct ggacttgcag aagcagctgg 901 gcagatttcc cggcaccTTC GTGGGCACCA CAGAGCCCGC CTCCCCACCC CTGAGCAGCA 961 CCTCACCCAC CACTGCTGCG GCCACTATGC CTGTGGTGCC CTCTGTGGCC AGCCTGGCCC 1021 CTCCAGGGGA GGCCTCGCTC TGCCTGGAAG AGGTGGCCCC CCCTGCCAGT GGGACCCGCA 1081 AAGCTCGGGT GCTCTATGAC TACGAGGCAG CCGACAGCAG TGAGCTGGCC CTGCTGGCTG 1141 ATGAGCTCAT CACTGTCTAC AGCCTGCCTG GCATGGACCC TGACTGGCTC ATTGGCGAGA 1201 GAGGCAACAA GAAGGGCAAG GTCCCTGTCA CCTACTTGGA ACTGCTCAGC TACCCAACTT 1261 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1321 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1381 CTTGTGGAAA GGACGATACA CTTGGTCCCT TGTGCAGTAC ACGCGTTAAG TCgacaatca 1441 acctctggat tacaaaattt gtgaaagatt