Construct: ORF ccsbBroadEn_03753

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000655.1_s300c1, BRDN0000397027
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SH3GLB2 (56904)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56904 SH3GLB2 SH3 domain containing GRB2 ... NM_001287046.1 99.9% 100% 960G>A
2 human 56904 SH3GLB2 SH3 domain containing GRB2 ... NM_020145.3 99.9% 100% 960G>A
3 human 56904 SH3GLB2 SH3 domain containing GRB2 ... XM_006717191.1 98.9% 98.9% 562_573del;972G>A
4 human 56904 SH3GLB2 SH3 domain containing GRB2 ... XM_005252098.2 98.6% 98.5% 840_854del;975G>A
5 human 56904 SH3GLB2 SH3 domain containing GRB2 ... NM_001287045.1 97.6% 97.5% 562_573del;852_866del;987G>A
6 human 56904 SH3GLB2 SH3 domain containing GRB2 ... NM_001369915.1 93.4% 93.2% 559_560ins63;777_791del;912G>A
7 human 56904 SH3GLB2 SH3 domain containing GRB2 ... XM_006717190.1 93.3% 93.3% 562_573del;636_707del;1044G>A
8 human 56904 SH3GLB2 SH3 domain containing GRB2 ... XM_006717189.2 93% 92.9% 624_695del;912_926del;1047G>A
9 human 56904 SH3GLB2 SH3 domain containing GRB2 ... NM_001369913.1 92.6% 90.6% (many diffs)
10 human 56904 SH3GLB2 SH3 domain containing GRB2 ... XM_006717188.2 92.2% 92% (many diffs)
11 human 56904 SH3GLB2 SH3 domain containing GRB2 ... NM_001369914.1 70.5% 74% 562_573del;849_850ins241;957_1098del
12 human 56904 SH3GLB2 SH3 domain containing GRB2 ... NR_163192.1 46.2% (many diffs)
13 human 56904 SH3GLB2 SH3 domain containing GRB2 ... XM_005252101.3 31.5% 31.6% 0_1ins810;150G>A
14 mouse 227700 Sh3glb2 SH3-domain GRB2-like endoph... NM_139302.2 90.5% 96.9% (many diffs)
15 mouse 227700 Sh3glb2 SH3-domain GRB2-like endoph... XM_006497937.1 89.6% 95.9% (many diffs)
16 mouse 227700 Sh3glb2 SH3-domain GRB2-like endoph... NM_001289709.1 89.4% 95.5% (many diffs)
17 mouse 227700 Sh3glb2 SH3-domain GRB2-like endoph... NM_001346806.1 88.5% 94.5% (many diffs)
18 mouse 227700 Sh3glb2 SH3-domain GRB2-like endoph... NM_001289710.1 85.3% 91.6% (many diffs)
19 mouse 227700 Sh3glb2 SH3-domain GRB2-like endoph... XM_006497938.1 84.7% 90.5% (many diffs)
20 mouse 227700 Sh3glb2 SH3-domain GRB2-like endoph... XM_006497939.1 84.2% 90.2% (many diffs)
21 mouse 227700 Sh3glb2 SH3-domain GRB2-like endoph... XM_011239078.1 75.7% 69.8% (many diffs)
22 mouse 227700 Sh3glb2 SH3-domain GRB2-like endoph... XM_011239077.1 74.8% 64.7% (many diffs)
23 mouse 227700 Sh3glb2 SH3-domain GRB2-like endoph... XM_006497941.3 66% 70.7% (many diffs)
24 mouse 227700 Sh3glb2 SH3-domain GRB2-like endoph... XM_006497942.3 66% 70.7% (many diffs)
25 mouse 227700 Sh3glb2 SH3-domain GRB2-like endoph... XR_001782060.1 36.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1251
ORF length:
1185
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGGA CTTCAACATG AAGAAGCTGG CGTCGGACGC GGGCATCTTC TTCACCCGGG
121CGGTGCAGTT CACGGAGGAG AAATTTGGCC AGGCTGAGAA GACTGAGCTT GATGCCCACT
181TTGAAAACCT TCTGGCCCGG GCAGACAGCA CCAAGAACTG GACAGAGAAG ATCTTGAGGC
241AGACAGAGGT GCTGCTGCAG CCCAACCCCA GTGCCCGAGT GGAGGAGTTC CTGTATGAGA
301AGCTGGACAG GAAGGTCCCC TCAAGGGTCA CCAACGGGGA GCTGCTGGCT CAGTACATGG
361CAGACGCGGC CAGTGAGCTG GGGCCGACCA CCCCCTATGG GAAGACACTG ATCAAGGTGG
421CAGAAGCTGA AAAGCAACTG GGAGCCGCGG AGAGGGATTT TATCCACACG GCCTCCATCA
481GCTTCCTCAC ACCCTTGCGC AACTTCCTGG AGGGGGACTG GAAGACCATC TCGAAGGAGA
541GGCGGCTCCT CCAAAACCGG CGTCTGGACT TGGATGCCTG CAAAGCGAGG CTGAAGAAGG
601CCAAGGCTGC AGAAGCCAAA GCCACGACGG TGCCTGACTT TCAGGAGACT AGACCTCGTA
661ATTACATTCT CTCGGCCAGC GCCTCCGCGC TCTGGAATGA TGAAGTGGAC AAGGCCGAGC
721AGGAGCTCCG CGTGGCCCAG ACAGAGTTTG ACCGGCAAGC AGAAGTGACC CGTCTCTTGC
781TGGAGGGAAT CAGTAGCACT CACGTGAACC ACCTGCGCTG CCTCCACGAG TTCGTCAAGT
841CTCAGACAAC CTACTACGCA CAGTGCTACC GCCACATGCT GGACTTGCAG AAGCAGCTGG
901GCAGATTTCC CGGCACCTTC GTGGGCACCA CAGAGCCCGC CTCCCCACCC CTGAGCAGCA
961CCTCACCCAC CACTGCTGCG GCCACTATGC CTGTGGTGCC CTCTGTGGCC AGCCTGGCCC
1021CTCCAGGGGA GGCCTCGCTC TGCCTGGAAG AGGTGGCCCC CCCTGCCAGT GGGACCCGCA
1081AAGCTCGGGT GCTCTATGAC TACGAGGCAG CCGACAGCAG TGAGCTGGCC CTGCTGGCTG
1141ATGAGCTCAT CACTGTCTAC AGCCTGCCTG GCATGGACCC TGACTGGCTC ATTGGCGAGA
1201GAGGCAACAA GAAGGGCAAG GTCCCTGTCA CCTACTTGGA ACTGCTCAGC TACCCAACTT
1261TCTTGTACAA AGTtggcatt ataagaaagc attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)