Transcript: Human NM_001396.4

Homo sapiens dual specificity tyrosine phosphorylation regulated kinase 1A (DYRK1A), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-26
Taxon:
Homo sapiens (human)
Gene:
DYRK1A (1859)
Length:
5475
CDS:
339..2630

Additional Resources:

NCBI RefSeq record:
NM_001396.4
NBCI Gene record:
DYRK1A (1859)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145031 TTACAGGAGTACAAACCACC pXPR_003 AGG 1250 55% 10 0.3388 DYRK1A DYRK1A 76755
2 BRDN0001148321 TCAGCAACCTCTAACTAACC pXPR_003 AGG 202 9% 3 0.2953 DYRK1A DYRK1A 76754
3 BRDN0001149496 TTCAACCAAAATACACCCGA pXPR_003 GGG 1042 45% 8 -0.0732 DYRK1A DYRK1A 76753
4 BRDN0001149042 TGAGAAACACCAATTTCCGA pXPR_003 GGG 762 33% 7 -0.5544 DYRK1A DYRK1A 76756
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001396.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000273284 GTTCGGCTTGCACCGTCATTT pLKO_005 381 CDS 100% 13.200 18.480 N DYRK1A n/a
2 TRCN0000022999 GCTGACTACTTGAAGTTCAAA pLKO.1 1677 CDS 100% 5.625 7.875 N Dyrk1a n/a
3 TRCN0000199464 GCACAGATAGAAGTGCGACTT pLKO.1 936 CDS 100% 4.050 5.670 N DYRK1A n/a
4 TRCN0000273347 GCACAGATAGAAGTGCGACTT pLKO_005 936 CDS 100% 4.050 5.670 N DYRK1A n/a
5 TRCN0000000523 CCAGCAGTTGATTCTTGTATT pLKO.1 4210 3UTR 100% 13.200 10.560 N DYRK1A n/a
6 TRCN0000321650 ATGGAGCTATGGACGTTAATT pLKO_005 2422 CDS 100% 15.000 10.500 N Dyrk1a n/a
7 TRCN0000273286 ATGTGGTCCCTCGGGTGTATT pLKO_005 1368 CDS 100% 13.200 9.240 N DYRK1A n/a
8 TRCN0000199188 CCTCTGCCGATAACCTGTTTA pLKO.1 4538 3UTR 100% 13.200 9.240 N DYRK1A n/a
9 TRCN0000273349 CCTCTGCCGATAACCTGTTTA pLKO_005 4538 3UTR 100% 13.200 9.240 N DYRK1A n/a
10 TRCN0000321714 TTGGTCATTTGCCAACTAATT pLKO_005 2952 3UTR 100% 13.200 9.240 N Dyrk1a n/a
11 TRCN0000197202 GAATGGAGCTATGGACGTTAA pLKO.1 2420 CDS 100% 10.800 7.560 N DYRK1A n/a
12 TRCN0000196506 GCTAAATTTGTGTTCCCATTT pLKO.1 4404 3UTR 100% 10.800 7.560 N DYRK1A n/a
13 TRCN0000010615 GATTCAGCAACCTCTAACTAA pLKO.1 521 CDS 100% 5.625 3.938 N DYRK1A n/a
14 TRCN0000195183 CCTGATATTGTCATGTTACAG pLKO.1 552 CDS 100% 4.950 3.465 N DYRK1A n/a
15 TRCN0000000527 CTTTGGACAGAATGGAGCTAT pLKO.1 2411 CDS 100% 4.950 3.465 N DYRK1A n/a
16 TRCN0000000524 GCTGCTAATACCTTGGACTTT pLKO.1 2394 CDS 100% 4.950 3.465 N DYRK1A n/a
17 TRCN0000010611 GCTGCTAATACCTTGGACTTT pLKO.1 2394 CDS 100% 4.950 3.465 N DYRK1A n/a
18 TRCN0000000525 CAGTATATTCAGAGTCGCTTT pLKO.1 1296 CDS 100% 4.050 2.835 N DYRK1A n/a
19 TRCN0000010612 CAGTATATTCAGAGTCGCTTT pLKO.1 1296 CDS 100% 4.050 2.835 N DYRK1A n/a
20 TRCN0000199259 CCCATTCACATCAGTACAGTG pLKO.1 442 CDS 100% 4.050 2.835 N DYRK1A n/a
21 TRCN0000000526 CGGAAGGTTTACAATGATGGT pLKO.1 735 CDS 100% 2.640 1.848 N DYRK1A n/a
22 TRCN0000010613 CGGAAGGTTTACAATGATGGT pLKO.1 735 CDS 100% 2.640 1.848 N DYRK1A n/a
23 TRCN0000273350 CGGAAGGTTTACAATGATGGT pLKO_005 735 CDS 100% 2.640 1.848 N DYRK1A n/a
24 TRCN0000010614 CAGGTTGTAAAGGCATATGAT pLKO.1 852 CDS 100% 0.000 0.000 N DYRK1A n/a
25 TRCN0000023001 CCTGCACATTACATGACTGAA pLKO.1 2520 CDS 100% 4.950 2.970 N Dyrk1a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001396.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14621 pDONR223 66.3% 96.9% 32.8% None (many diffs) n/a
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