Construct: ORF TRCN0000465580
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000256.1_s317c1
- Derived from:
- ccsbBroadEn_06149
- DNA Barcode:
- TAACTTGACGAAAGGACCCTGTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF4B (1975)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465580
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1975 | EIF4B | eukaryotic translation init... | NM_001417.7 | 99.8% | 100% | 1644C>A;1755C>T |
| 2 | human | 1975 | EIF4B | eukaryotic translation init... | NM_001300821.3 | 99% | 99% | 1307_1321del;1659C>A;1770C>T |
| 3 | human | 1975 | EIF4B | eukaryotic translation init... | NM_001330654.2 | 93.5% | 93.6% | 360_361ins117;1527C>A;1638C>T |
| 4 | mouse | 75705 | Eif4b | eukaryotic translation init... | NM_145625.3 | 90% | 94.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1899
- ORF length:
- 1833
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcctcagca aaaaagaaga ataagaaggg gaagactatc tccctaacag 121 actttctggc tgaggatggg ggtactggtg gaggaagcac ctatgtttcc aaaccagtca 181 gctgggctga tgaaacggat gacctggaag gagatgtttc gaccacttgg cacagtaacg 241 atgacgatgt gtatagggcg cctccaattg accgttccat ccttcccact gctccacggg 301 ctgctcggga acccaatatc gaccggagcc gtcttcccaa atcgccaccc tacactgctt 361 ttctaggaaa cctaccctat gatgttacag aagagtcaat taaggaattc tttcgaggat 421 taaatatcag tgcagtgcgt ttaccacgtg aacccagcaa tccagagagg ttgaaaggtt 481 ttggttatgc tgaatttgag gacctggatt ccctgctcag tgccctgagt ctcaatgaag 541 agtctctagg taacaggaga attcgagtgg acgttgctga tcaagcacag gataaagaca 601 gggatgatcg ttcttttggc cgtgatagaa atcgggattc tgacaaaaca gatacagact 661 ggagggctcg tcctgctaca gacagctttg atgactaccc acctagaaga ggtgatgata 721 gctttggaga caagtatcga gatcgttatg attcagaccg gtatcgggat gggtatcggg 781 atgggtatcg ggatggccca cgccgggata tggatcgata tggtggccgg gatcgctatg 841 atgaccgagg cagcagagac tatgatagag gctatgattc ccggataggc agtggcagaa 901 gagcatttgg cagtgggtat cgcagggatg atgactacag aggaggcggg gaccgctatg 961 aagaccgata tgacagacgg gatgatcggt cgtggagctc cagagatgat tactctcggg 1021 atgattatag gcgtgatgat agaggtcccc cccaaagacc caaactgaat ctaaagcctc 1081 ggagtactcc taaggaagat gattcctctg ctagtacctc ccagtccact cgagctgctt 1141 ctatctttgg aggggcaaag cctgttgaca cagctgctag agaaagagaa gtagaagaac 1201 ggctacagaa ggaacaagag aagttgcagc gtcagctgga tgagccaaaa ctagaacgac 1261 ggcctcggga gagacaccca agctggcgaa gtgaagaaac tcaggaacgg gaacggtcga 1321 ggacaggaag tgagtcatca caaactggga cctccaccac atctagcaga aatgcacgaa 1381 ggagagagag tgagaagtct ctagaaaatg aaacactcaa taaggaggaa gattgccact 1441 ctccaacttc taaacctccc aaacctgatc agcccctaaa ggtaatgcca gcccctccac 1501 caaaggagaa tgcttgggtg aagcgaagtt ctaaccctcc tgctcgatct cagagctcag 1561 acacagagca gcagtcccct acaagtggtg ggggaaaagt agctccagct caaccatcTG 1621 AGGAAGGACC AGGAAGGAAA GATGAAAATA AAGTAGATGG GATGAATGCC CCAAAAGGCC 1681 AAACTGGGAA CTCTAGCCGT GGTCCAGGAG ACGGAGGGAA CAGAGACCAC TGGAAGGAGT 1741 CAGATAGGAA AGATGGCAAA AAGGATCAAG ACTCCAGATC TGCACCTGAG CCAAAGAAAC 1801 CTGAGGAAAA TCCAGCTTCT AAGTTCAGTT CTGCAAGCAA GTATGCTGCT CTCTCTGTTG 1861 ATGGTGAAGA TGAAAATGAG GGAGAAGATT ATGCCGAATA CCCAACTTTC TTGTACAAAG 1921 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1981 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2041 ACGATAACTT GACGAAAGGA CCCTGTGCAC GCGTTAAGTC gacaatcaac ctctggatta 2101 caaaatttgt gaaagatt