Transcript: Human NM_003809.3

Homo sapiens TNF superfamily member 12 (TNFSF12), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
TNFSF12 (8742)
Length:
1377
CDS:
97..846

Additional Resources:

NCBI RefSeq record:
NM_003809.3
NBCI Gene record:
TNFSF12 (8742)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_003809.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000373713 CCTCACCTACTTCGGACTCTT pLKO_005 813 CDS 100% 4.950 6.930 N TNFSF12 n/a
2 TRCN0000373710 GTATTCCCACTCTTATCTTAC pLKO_005 1015 3UTR 100% 10.800 8.640 N TNFSF12 n/a
3 TRCN0000373712 GGGCATTGTGTTCACTGTACT pLKO_005 1134 3UTR 100% 4.950 3.960 N TNFSF12 n/a
4 TRCN0000373711 CTGTACTGTCAGGTGCACTTT pLKO_005 583 CDS 100% 4.950 3.465 N TNFSF12 n/a
5 TRCN0000058149 GCAGCCCATTATGAAGTTCAT pLKO.1 418 CDS 100% 4.950 2.475 Y TNFSF12 n/a
6 TRCN0000204191 GCAGCCCATTATGAAGTTCAT pLKO.1 418 CDS 100% 4.950 2.475 Y TNFSF12-TNFSF13 n/a
7 TRCN0000058151 CAGACAGAAGAAAGCCAGGAT pLKO.1 316 CDS 100% 2.640 1.320 Y TNFSF12 n/a
8 TRCN0000058150 CCTTTCCTGAACCGACTAGTT pLKO.1 343 CDS 100% 0.000 0.000 Y TNFSF12 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_003809.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07293 pDONR223 99.4% 100% 100% None n/a
2 ccsbBroad304_07293 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000475473 TACGTGCAGATCGTGCACATACGC pLX_317 29.7% 100% 100% V5 n/a
4 ccsbBroadEn_10229 pDONR223 100% 52.3% 50.6% None (many diffs) n/a
5 ccsbBroad304_10229 pLX_304 0% 52.3% 50.6% V5 (many diffs) n/a
6 TRCN0000471257 ATGGTCCTTTGTTTTTTGCTAACA pLX_317 35.7% 52.3% 50.6% V5 (many diffs) n/a
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