Transcript: Mouse NM_007961.4

Mus musculus ets variant 6 (Etv6), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-04
Taxon:
Mus musculus (mouse)
Gene:
Etv6 (14011)
Length:
5548
CDS:
407..1864

Additional Resources:

NCBI RefSeq record:
NM_007961.4
NBCI Gene record:
Etv6 (14011)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_007961.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231234 GCATTAAGCAGGAACGAATTT pLKO_005 432 CDS 100% 13.200 18.480 N Etv6 n/a
2 TRCN0000003853 GCGCCACTACTACAAACTAAA pLKO.1 1588 CDS 100% 13.200 10.560 N ETV6 n/a
3 TRCN0000338363 GCGCCACTACTACAAACTAAA pLKO_005 1588 CDS 100% 13.200 10.560 N ETV6 n/a
4 TRCN0000231236 CAGTGTCTCCTGTCGAGAATA pLKO_005 1119 CDS 100% 13.200 9.240 N Etv6 n/a
5 TRCN0000257202 CTGCGTTTGCAGCCGATTTAC pLKO_005 569 CDS 100% 13.200 9.240 N Etv6 n/a
6 TRCN0000231235 TCAGAACCATGACGAAGATAA pLKO_005 856 CDS 100% 13.200 9.240 N Etv6 n/a
7 TRCN0000231237 TCGACATTAAAGCGACATTAA pLKO_005 4300 3UTR 100% 13.200 9.240 N Etv6 n/a
8 TRCN0000084402 GCTGCTGACCAAAGAGGATTT pLKO.1 694 CDS 100% 10.800 7.560 N Etv6 n/a
9 TRCN0000003856 CCATAAGAACAGAACAAACAT pLKO.1 1540 CDS 100% 5.625 3.938 N ETV6 n/a
10 TRCN0000338303 CCATAAGAACAGAACAAACAT pLKO_005 1540 CDS 100% 5.625 3.938 N ETV6 n/a
11 TRCN0000084400 CCGATTTACTGGAGCAGAGAT pLKO.1 581 CDS 100% 4.950 3.465 N Etv6 n/a
12 TRCN0000084398 CGCTTCTCCTTCTGATCTCTT pLKO.1 1982 3UTR 100% 4.950 3.465 N Etv6 n/a
13 TRCN0000084399 CCTGGCTTACTTGAACCACAT pLKO.1 1327 CDS 100% 4.050 2.835 N Etv6 n/a
14 TRCN0000084401 CTGCATCAGAACCATGACGAA pLKO.1 851 CDS 100% 2.640 1.848 N Etv6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007961.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00522 pDONR223 100% 81.4% 81.9% None (many diffs) n/a
2 TRCN0000475139 CCTCCGCACGACCGATTCAGTGTC pLX_317 21.2% 81.4% 81.9% V5 (many diffs) n/a
3 ccsbBroad304_00522 pLX_304 51.9% 76.4% 45.3% V5 (not translated due to prior stop codon) (many diffs) n/a
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