Transcript: Mouse NM_013602.3

Mus musculus metallothionein 1 (Mt1), mRNA.

Source:
NCBI, updated 2017-06-25
Taxon:
Mus musculus (mouse)
Gene:
Mt1 (17748)
Length:
583
CDS:
223..408

Additional Resources:

NCBI RefSeq record:
NM_013602.3
NBCI Gene record:
Mt1 (17748)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_013602.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000219455 TCCCGTGGGCTGCTCCAAATG pLKO.1 333 CDS 100% 0.000 0.000 N Mt1 n/a
2 TRCN0000219451 CCTGCAAGAACTGCAAGTGCA pLKO.1 281 CDS 100% 2.640 1.848 N Mt1 n/a
3 TRCN0000219453 CAAATGTGCCCAGGGCTGTGT pLKO.1 348 CDS 100% 0.880 0.616 N Mt1 n/a
4 TRCN0000219452 AGTGCACCTCCTGCAAGAAGA pLKO.1 296 CDS 100% 4.950 2.970 N Mt1 n/a
5 TRCN0000242865 CCTGCAAGAAGAGCTGCTGCT pLKO_005 305 CDS 100% 0.720 0.432 N MT1G n/a
6 TRCN0000219454 CTGCAAGAAGAGCTGCTGCTC pLKO.1 306 CDS 100% 0.720 0.360 Y Mt1 n/a
7 TRCN0000364945 CCTGCAAGAAGAGCTGCTGTT pLKO_005 305 CDS 100% 4.050 2.430 N MT1E n/a
8 TRCN0000242659 AGAAGAGCTGCTGCTCCTGTT pLKO_005 311 CDS 100% 4.050 2.025 Y MT1H n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_013602.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01040 pDONR223 100% 85.7% 85.2% None (many diffs) n/a
2 ccsbBroad304_01040 pLX_304 0% 85.7% 85.2% V5 (many diffs) n/a
3 TRCN0000465872 GTAGAGTCACTGCCTTGTCCTAGG pLX_317 100% 85.7% 85.2% V5 (many diffs) n/a
4 ccsbBroadEn_01042 pDONR223 100% 85.3% 78.6% None (many diffs) n/a
5 ccsbBroad304_01042 pLX_304 0% 85.3% 78.6% V5 (many diffs) n/a
6 TRCN0000470527 TTTTTAACCATTGCTGGCACAAAG pLX_317 100% 85.3% 78.6% V5 (many diffs) n/a
7 ccsbBroadEn_13207 pDONR223 100% 83% 80.3% None (many diffs) n/a
8 ccsbBroad304_13207 pLX_304 0% 83% 80.3% V5 (many diffs) n/a
9 TRCN0000467056 AGATTTTCATGCAACTTTTCTCGA pLX_317 100% 83% 80.3% V5 (many diffs) n/a
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