Construct: ORF TRCN0000465872
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009744.1_s317c1
- Derived from:
- ccsbBroadEn_01040
- DNA Barcode:
- GTAGAGTCACTGCCTTGTCCTAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MT1E (4493)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465872
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4493 | MT1E | metallothionein 1E | NM_175617.4 | 100% | 100% | |
| 2 | human | 4489 | MT1A | metallothionein 1A | NM_005946.3 | 94.5% | 93.4% | (many diffs) |
| 3 | human | 4495 | MT1G | metallothionein 1G | NM_005950.3 | 94.5% | 93.4% | (many diffs) |
| 4 | human | 4495 | MT1G | metallothionein 1G | NM_001301267.2 | 93% | 91.9% | (many diffs) |
| 5 | human | 4496 | MT1H | metallothionein 1H | NM_005951.2 | 92.8% | 90.1% | (many diffs) |
| 6 | human | 4502 | MT2A | metallothionein 2A | NM_005953.5 | 92.3% | 93.4% | (many diffs) |
| 7 | human | 645745 | MT1HL1 | metallothionein 1H like 1 | NM_001276687.2 | 92.3% | 88.5% | (many diffs) |
| 8 | human | 4499 | MT1M | metallothionein 1M | NM_176870.3 | 92.3% | 85.2% | (many diffs) |
| 9 | human | 4494 | MT1F | metallothionein 1F | NM_005949.4 | 91.8% | 93.4% | (many diffs) |
| 10 | human | 4490 | MT1B | metallothionein 1B | NM_005947.3 | 91.2% | 91.8% | (many diffs) |
| 11 | human | 4501 | MT1X | metallothionein 1X | NM_005952.4 | 90.7% | 88.5% | (many diffs) |
| 12 | human | 4494 | MT1F | metallothionein 1F | NM_001301272.1 | 60.6% | 52.3% | (many diffs) |
| 13 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_027781.1 | 44.1% | (many diffs) | |
| 14 | human | 4498 | MT1JP | metallothionein 1J, pseudogene | NR_036677.1 | 42.1% | (many diffs) | |
| 15 | human | 4500 | MT1L | metallothionein 1L, pseudogene | NR_001447.2 | 39% | (many diffs) | |
| 16 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_003669.2 | 36.4% | (many diffs) | |
| 17 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_104045.1 | 33.1% | (many diffs) | |
| 18 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_003658.2 | 31.3% | (many diffs) | |
| 19 | mouse | 17750 | Mt2 | metallothionein 2 | NM_008630.2 | 86.3% | 85.2% | (many diffs) |
| 20 | mouse | 17748 | Mt1 | metallothionein 1 | NM_013602.3 | 85.7% | 85.2% | (many diffs) |
| 21 | mouse | 17748 | Mt1 | metallothionein 1 | XM_006530752.3 | 39.6% | 39.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 273
- ORF length:
- 183
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGACCCCAA CTGCTCTTGC GCCACTGGTG 121 GCTCCTGCAC GTGCGCCGGC TCCTGCAAGT GCAAAGAGTG CAAATGCACC TCCTGCAAGA 181 AGAGCTGCTG TTCCTGCTGC CCCGTGGGCT GTGCCAAGTG TGCCCAGGGC TGCGTCTGCA 241 AAGGGGCATC GGAGAAGTGC AGCTGCTGTG CCTGCCCGAC TTTCTTGTAC AAAGTGGTTG 301 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 361 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGT 421 AGAGTCACTG CCTTGTCCTA GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 481 ttgtgaaaga tt