Construct: ORF TRCN0000470527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007199.1_s317c1
- Derived from:
- ccsbBroadEn_01042
- DNA Barcode:
- TTTTTAACCATTGCTGGCACAAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MT1M (4499)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4499 | MT1M | metallothionein 1M | NM_176870.3 | 99.4% | 98.3% | 59C>A |
| 2 | human | 4493 | MT1E | metallothionein 1E | NM_175617.4 | 92.8% | 86.8% | (many diffs) |
| 3 | human | 4489 | MT1A | metallothionein 1A | NM_005946.3 | 91.8% | 83.6% | (many diffs) |
| 4 | human | 4495 | MT1G | metallothionein 1G | NM_005950.3 | 91.8% | 83.6% | (many diffs) |
| 5 | human | 4490 | MT1B | metallothionein 1B | NM_005947.3 | 91.2% | 86.8% | (many diffs) |
| 6 | human | 4494 | MT1F | metallothionein 1F | NM_005949.4 | 91.2% | 83.6% | (many diffs) |
| 7 | human | 4496 | MT1H | metallothionein 1H | NM_005951.2 | 91.2% | 81.9% | (many diffs) |
| 8 | human | 4501 | MT1X | metallothionein 1X | NM_005952.4 | 90.7% | 83.6% | (many diffs) |
| 9 | human | 4495 | MT1G | metallothionein 1G | NM_001301267.2 | 90.3% | 82.2% | (many diffs) |
| 10 | human | 4502 | MT2A | metallothionein 2A | NM_005953.5 | 90.1% | 81.9% | (many diffs) |
| 11 | human | 645745 | MT1HL1 | metallothionein 1H like 1 | NM_001276687.2 | 90.1% | 78.6% | (many diffs) |
| 12 | human | 4494 | MT1F | metallothionein 1F | NM_001301272.1 | 60.6% | 49.2% | (many diffs) |
| 13 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_027781.1 | 42.8% | (many diffs) | |
| 14 | human | 4498 | MT1JP | metallothionein 1J, pseudogene | NR_036677.1 | 41.9% | (many diffs) | |
| 15 | human | 4500 | MT1L | metallothionein 1L, pseudogene | NR_001447.2 | 38.1% | (many diffs) | |
| 16 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_003669.2 | 36.7% | (many diffs) | |
| 17 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_104045.1 | 33.4% | (many diffs) | |
| 18 | mouse | 17748 | Mt1 | metallothionein 1 | NM_013602.3 | 85.3% | 78.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 226
- ORF end:
- 409
- ORF length:
- 183
- Sequence:
-
1 GGGGGGAGGG GTTTTATGCG ATGGAGTTTC CCCACACTGA GTGGGTGGAG ACTGAAGTTA 61 GGCCAGCTTG GCACTTGATG TAATTCTCCT TGGAATTTGC CCTTTTTGAG TTTGGATCTT 121 GGTTCATTCT CAAGCCTCAG ACAGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT 181 GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG TTGGCATGGA CCCCAACTGC 241 TCCTGCACCA CTGGTGTCTC CTGCGCCTGC ACCGGCTCCT GCAAGTGCAA AGAGTGCAAA 301 TGCACCTCCT GCAAGAAGAG CTGCTGCTCC TGCTGCCCCG TGGGCTGTGC CAAGTGTGCC 361 CACGGCTGTG TCTGCAAAGG GACGTTGGAG AACTGCAGCT GCTGTGCCTG CCCAACTTTC 421 TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT 481 ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 541 TGTGGAAAGG ACGATTTTTA ACCATTGCTG GCACAAAGAC GCGTTAAGTC gacaatcaac 601 ctctggatta caaaatttgt gaaagatt