Construct: ORF TRCN0000467056
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008668.1_s317c1
- Derived from:
- ccsbBroadEn_13207
- DNA Barcode:
- AGATTTTCATGCAACTTTTCTCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MT1P3 (140851)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467056
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4493 | MT1E | metallothionein 1E | NM_175617.4 | 91.8% | 90.1% | (many diffs) |
| 2 | human | 4489 | MT1A | metallothionein 1A | NM_005946.3 | 90.1% | 86.8% | (many diffs) |
| 3 | human | 4499 | MT1M | metallothionein 1M | NM_176870.3 | 90.1% | 81.9% | (many diffs) |
| 4 | human | 4494 | MT1F | metallothionein 1F | NM_005949.4 | 88.5% | 85.2% | (many diffs) |
| 5 | human | 4495 | MT1G | metallothionein 1G | NM_005950.3 | 88.5% | 83.6% | (many diffs) |
| 6 | human | 4496 | MT1H | metallothionein 1H | NM_005951.2 | 88.5% | 83.6% | (many diffs) |
| 7 | human | 4490 | MT1B | metallothionein 1B | NM_005947.3 | 87.9% | 85.2% | (many diffs) |
| 8 | human | 645745 | MT1HL1 | metallothionein 1H like 1 | NM_001276687.2 | 87.9% | 80.3% | (many diffs) |
| 9 | human | 4502 | MT2A | metallothionein 2A | NM_005953.5 | 87.4% | 85.2% | (many diffs) |
| 10 | human | 4495 | MT1G | metallothionein 1G | NM_001301267.2 | 87% | 82.2% | (many diffs) |
| 11 | human | 4501 | MT1X | metallothionein 1X | NM_005952.4 | 86.8% | 81.9% | (many diffs) |
| 12 | human | 4494 | MT1F | metallothionein 1F | NM_001301272.1 | 64.8% | 55% | (many diffs) |
| 13 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_027781.1 | 42% | (many diffs) | |
| 14 | human | 4498 | MT1JP | metallothionein 1J, pseudogene | NR_036677.1 | 40.6% | (many diffs) | |
| 15 | human | 4500 | MT1L | metallothionein 1L, pseudogene | NR_001447.2 | 37.2% | (many diffs) | |
| 16 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_003669.2 | 35.7% | (many diffs) | |
| 17 | human | 644314 | MT1IP | metallothionein 1I, pseudogene | NR_104045.1 | 32.4% | (many diffs) | |
| 18 | human | 326343 | MT1DP | metallothionein 1D, pseudogene | NR_003658.2 | 29.9% | (many diffs) | |
| 19 | mouse | 17748 | Mt1 | metallothionein 1 | NM_013602.3 | 83% | 80.3% | (many diffs) |
| 20 | mouse | 17750 | Mt2 | metallothionein 2 | NM_008630.2 | 81.9% | 77% | (many diffs) |
| 21 | mouse | 17748 | Mt1 | metallothionein 1 | XM_006530752.3 | 38.3% | 37.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 216
- ORF end:
- 390
- ORF length:
- 174
- Sequence:
-
1 TTATGCGATG GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA 61 CTTGATGTAA TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA 121 GCCTCAGACA GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GCTAGCATCG 181 ATTGATCAAC AAGTTTGTAC AAAAAAGTTG GCACCATGGA CCCCAACTGC TCCTGCACCA 241 CTGGTGGCTC CTGCACGTGC GCCGGCTCCT GCAAGTGCAA AGAGTGCAAA TGCACCTCCT 301 GCAAGAAGAG CTGCTGCTCC TGCTGCCCCA TGGGCTGTGC CAAGTGTGCC CAGGGCTGTG 361 TCTGCAAAGG GGCGTGCAGC TGCTGTGTCT TGCCAACTTT CTTGTACAAA GTGGTTGATA 421 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 481 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGATT 541 TTCATGCAAC TTTTCTCGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 601 tgaaagatt